黄连土壤阿魏酸降解菌的筛选及降解特性
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摘要
为筛选黄连土壤来源的阿魏酸降解菌,从黄连种植基地的土壤中分离能够在以阿魏酸为唯一碳源的培养基中生长的真菌,ITS序列比对鉴定种属,采用高效液相色谱法检测菌株在不同条件下对阿魏酸的降解能力.结果表明:获得对阿魏酸具有降解能力的真菌F-0056,鉴定为齿孢青霉(Penicillium daleae);外源阿魏酸添加量为200μg/mL时,菌株F-0056培养72h后阿魏酸降解率达87.5%;真菌F-0056在土壤环境下144h对阿魏酸降解效率达71.8%.
In order to screen ferulic acid-degrading microbes from rhizosphere soil of Coptis chinensis,a fungus strain which could grow in a medium with ferulic acid as the sole carbon source was isolated from the soil of a C.chinensis plantation.With ITS(internal transcribed spacer)sequence analysis this strain(F-0056)was identified as Penicillium daleae.High performance liquid chromatography(HPLC)was used to determine its degradation ability of ferulic acid under different conditions,and the results showed that the ferulic acid degradation rate of F-0056 reached 87.5%after 72hours'culture in a medium supplemented with ferulic acid at 200μg/mL and 71.8%in a soil environment ofter 144 hours.
In order to screen ferulic acid-degrading microbes from rhizosphere soil of Coptis chinensis,a fungus strain which could grow in a medium with ferulic acid as the sole carbon source was isolated from the soil of a C.chinensis plantation.With ITS(internal transcribed spacer)sequence analysis this strain(F-0056)was identified as Penicillium daleae.High performance liquid chromatography(HPLC)was used to determine its degradation ability of ferulic acid under different conditions,and the results showed that the ferulic acid degradation rate of F-0056 reached 87.5%after 72hours'culture in a medium supplemented with ferulic acid at 200μg/mL and 71.8%in a soil environment ofter 144 hours.
引文
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[3] CHEN Shao-li,ZHOU Bao-li,LIN Shan-shan,et al.Accumulation of Cinnamic Acid and Vanillin in Eggplant Root Exudates and the Relationship with Continuous Cropping Obstacle[J].African Journal of Biotechnology,2011,10(14):2659-2665.
[4] DONG Lin-lin,XU Jiang,FENG Guangquan,et al.Soil Bacterial and Fungal Community Dynamics in Relation to Panax notoginseng Death Rate in a Continuous Cropping System[J].Scientific Reports,2016,6(31802):1-11.
[5] KOLE R K,KARMAKAR P R,POI R,et al.Allelopathic Inhibition of Teak Leaf Extract:a Potential Pre-Emergent Herbicide[J].Journal of Crop and Weed,2011,7(1):101-109.
[6] YAN Jian,BI Hai-hong,LIU Yong-zhu,et al.Phenolic Compounds from Merremia umbellata subsp.orientalis and Their Allelopathic Effects on Arabidopsis Seed Germination[J].Molecules,2010,15(11):8241-8250.
[7]张丹,钟国跃,曹纬国,等.重庆黄连种植土壤中酚酸类物质累积与消减规律的研究[J].世界科学技术—中医药现代化,2015,17(7):1419-1424.
[8]李阳波,何林卫,张薇,等.黄连须根浸提液对土壤微生物及酶活性的影响[J].中国中药杂志,2014,39(21):4205-4210.
[9] FIERER N.Embracing the Unknown:Disentangling the Complexities of the Soil Microbiome[J].Nature Reviews Microbiology,2017,15(10):579-590.
[10]ALRUMMAN S A,STANDING D B,PATON G I.Effects of Hydrocarbon Contamination on Soil Microbial Community and Enzyme Activity[J].Journal of King Saud University Science,2015,27(1):31-41.
[11]QH X H,WANG JingGuo.Effect of Amendments with Different Phenolic Acids on Soil Microbial Biomass,Activity,and Community Diversity[J].Applied Soil Ecology,2008,39(2):172-179.
[12]徐海燕,雷世梅,熊伟,等.丛枝菌根化枳橙根际微生态环境的研究[J].西南大学学报(自然科学版),2012,34(10):65-71.
[13]SANTHANAM R,LUU V T,WEINHOLD A,et al.Native Root-Associated Bacteria Rescue a Plant from a SuddenWilt Disease That Emerged During Continuous Cropping[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(36):E5013-E5020.
[14]张一迪.甜瓜自毒物质降解菌的筛选及其降解作用研究[D].沈阳:沈阳农业大学,2018.
[15]FAVRE P,BAPAUME L,BOSSOLINI E,et al.A Novel Bioinformatics Pipeline to Discover Genes Related to Arbuscular Mycorrhizal Symbiosis Based on Their Evolutionary Conservation Pattern Among Higher Plants[J].BMC Plant Biology,2014,14(333):1-20.
[16]徐淑霞,张世敏,尤晓颜,等.黄孢原毛平革菌对黄瓜连作土壤酚酸物质的降解[J].应用生态学报,2008,19(11):2480-2484.
[17]赵智灵,刘学周,魏晓雨,等.人参可利用内生菌株的筛选和鉴定[J].中草药,2015,46(14):2143-2148.