逍遥合剂对产后抑郁症模型大鼠行为学、神经递质及ERK1/2通路影响的研究
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摘要
目的:探讨逍遥合剂对产后抑郁症模型大鼠行为学、神经递质及ERK1/2通路的影响。方法:SPF级雌性SD大鼠共72只,随机分正常组、模型组(生理盐水1ml/100g)、逍遥合剂高、中、低剂量组即7、3.6、1.8g(kg·d)、阳性药组用盐酸氟西汀组10mg/(kg·d),每组有12只。各模型组肌注苯甲酸雌二醇及黄体酮以建立产后抑郁症模型大鼠,共造模23d。造模成功后予各治疗组灌胃药物,正常组和模型组灌胃生理盐水,观察各组大鼠体重、行为学、神经递质及ERK1/2蛋白表达情况。结果:3周后,各治疗组大鼠体重均高于模型组体重,且正常组、逍遥合剂高、中剂量组显著高于模型组(P<0.05),逍遥合剂的高剂量组体重显著高于盐酸氟西汀组(P<0.05)。治疗前各治疗组大鼠行为学指标评分均无显著差异(P>0.05),治疗后逍遥合剂高、中剂量组及盐酸氟西汀组的水平移动总路程较模型组有统计学意义(P<0.05),逍遥合剂高、中、低剂量组及盐酸氟西汀组的中央区域路程较模型组有统计学意义(P<0.05)。逍遥合剂高、中剂量组5-HT、DA、NE水平较模型组显著上升(P<0.05)。逍遥合剂高、中剂量组p-CREB、p-ERK1/2蛋白水平较模型组显著上升(P<0.05),逍遥合剂低剂量组及盐酸氟西汀组较逍遥合剂高剂量组显著降低(P<0.05)。结论:逍遥合剂能有效改善产后抑郁模型大鼠行为学各项指标,可能是通过上调下丘脑神经递质及ERK1/2信号通路上p-MEK1、MEK1蛋白水平来缓解抑郁症状。
Objective:To investigate the effects of Xiaoyao mixture on behavior,neurotransmitter and ERK1/2 pathway in postnatal depression rats.Medthods:A total of 72 SPF female SD rats were randomly divided into normal group,model group(Normal saline 1 ml/100 g),Xiaoyao mixture high,middle and low dose group 7,3.6,1.8 g/(kg·d)and positive drug group fluoxetine hydrochloride group 10 mg/(kg·d)with 12 rats in each group.The rats in each group were injected with estradiol benzoate and progesterone to establish postnatal depression model rats for23 days.The normal group and the model group were given the normal saline,and the body weight,behavior,neurotransmitters and ERK1/2 protein expression were observed in the normal group and the model group.Results:After 3 weeks,the weight of the rats in the treatment group was higher than that of the model group,and the normal group,the Xiaoyao mixture high and middle dose group were significantly higher than the model group(P<0.05).The high dose group of Xiaoyao mixture was significantly higher than that of the fluoxetine hydrochloride group(P<0.05).Before treatment,there was no significant difference(P>0.05).After treatment,the total distance movement of Xiaoyao mixture high,middle dose group and fluoxetine hydrochloride group was significantly different than that of the model group(P<0.05).The central regional route of Xiaoyao mixture high,middle and low dosage groups and fluoxetine hydrochloride group was more than that of the model group.There were significant differences in the model group(P<0.05).The levels of 5-HT,DA and NE in the high and middle dose groups of Xiaoyao mixture were significantly higher than those in the model group(P<0.05).The level of p-CREB and p-ERK1/2 protein in the high and middle dose groups of Xiaoyao mixture was significantly higher than that in the model group(P<0.05).The low dose group and the high dose group of the fluoxetine hydrochloride group were significantly lower than those in the high dose group(P<0.05).Conclusion:Xiaoyao mixture can effectively improve the behavior of postpartum depression model rats,possibly by raising the level of p-MEK1 and MEK1 protein in the hypothalamus neurotransmitter and ERK1/2 signaling pathway to alleviate the depressive symptoms.
Objective:To investigate the effects of Xiaoyao mixture on behavior,neurotransmitter and ERK1/2 pathway in postnatal depression rats.Medthods:A total of 72 SPF female SD rats were randomly divided into normal group,model group(Normal saline 1 ml/100 g),Xiaoyao mixture high,middle and low dose group 7,3.6,1.8 g/(kg·d)and positive drug group fluoxetine hydrochloride group 10 mg/(kg·d)with 12 rats in each group.The rats in each group were injected with estradiol benzoate and progesterone to establish postnatal depression model rats for23 days.The normal group and the model group were given the normal saline,and the body weight,behavior,neurotransmitters and ERK1/2 protein expression were observed in the normal group and the model group.Results:After 3 weeks,the weight of the rats in the treatment group was higher than that of the model group,and the normal group,the Xiaoyao mixture high and middle dose group were significantly higher than the model group(P<0.05).The high dose group of Xiaoyao mixture was significantly higher than that of the fluoxetine hydrochloride group(P<0.05).Before treatment,there was no significant difference(P>0.05).After treatment,the total distance movement of Xiaoyao mixture high,middle dose group and fluoxetine hydrochloride group was significantly different than that of the model group(P<0.05).The central regional route of Xiaoyao mixture high,middle and low dosage groups and fluoxetine hydrochloride group was more than that of the model group.There were significant differences in the model group(P<0.05).The levels of 5-HT,DA and NE in the high and middle dose groups of Xiaoyao mixture were significantly higher than those in the model group(P<0.05).The level of p-CREB and p-ERK1/2 protein in the high and middle dose groups of Xiaoyao mixture was significantly higher than that in the model group(P<0.05).The low dose group and the high dose group of the fluoxetine hydrochloride group were significantly lower than those in the high dose group(P<0.05).Conclusion:Xiaoyao mixture can effectively improve the behavior of postpartum depression model rats,possibly by raising the level of p-MEK1 and MEK1 protein in the hypothalamus neurotransmitter and ERK1/2 signaling pathway to alleviate the depressive symptoms.
引文
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[13]李春雨,刘梦,谢萍.补虚化瘀法对产后抑郁低雌激素大鼠海马核糖体S6激酶和丝裂原活化细胞外调节激酶1的影响[J].科学技术与工程,2018,27(7):35-38.
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[15]张锦花,洪兰,吕学玉,等.低阻抗意念导入疗法对抑郁症患者外周神经递质的影响[J].国际中医中药杂志,2016,38(10):895-897.
[16]唐文娟,钱一平,顾康莹,等.联合综合心理干预对复发性抑郁症患者抑郁情绪、生命健康质量及神经递质的影响[J].中国医药导报,2017,14(34):25-28.
[17]荣晅,彭建美,宋宇,等.探讨二甲双胍对多囊卵巢综合征患者的疗效及其心理健康和血清单胺类神经递质浓度的关系[J].国际精神病学杂志,2017,30(5):867-869.
[18]呼日乐巴根,松林,乌兰其其格,等.肉蔻五味丸对慢性应激性抑郁症大鼠学习记忆能力及海马BDNF、TrkB蛋白表达的影响[J].中华中医药杂志,2017,26(8):3797-3800.
[2]包春荣,余自成,冯轶杉,等.产后抑郁症治疗新进展[J].世界临床药物,2016,23(2):135-139.
[3]朱伟,冯青.马来酸氟伏沙明对抑郁患者抑郁症状焦虑症状及生活质量的影响[J].山西医药杂志,2017,46(21):2641-2643.
[4]王东明,刘琳琳,孙平,等.小柴胡汤改善抑郁症状及相关神经递质与神经营养因子分析[J].国际精神病学杂志,2016,32(1):100-103.
[5]袁丽.百合知母汤的抗抑郁作用及其ERK1/2、CaMK信号通路机制[D].武汉:湖北中医药大学,2016.
[6]尹航,夏爱斌,雷俊.多不饱和脂肪酸、维生素D及同型半胱氨酸与产后抑郁关系的研究进展[J].中南大学学报:医学版,2016,41(10):1096-1100.
[7]王丹,李小黎,赵瑞珍,等.参芪解郁方对产后抑郁症患者雌、孕激素及EPDS评分的影响[J].北京中医药,2016,26(1):29-32.
[8]侯玉敏.补虚化瘀法对产后抑郁大鼠雌激素,p-CREB,pERK1/2蛋白表达水平的影响附:雌激素与产后抑郁关系的研究现状[D].成都:成都中医药大学,2015.
[9]宁海燕,谢萍,冯俭,等.产后抑郁大鼠模型的构建及参归仁合剂对其抑郁样行为的影响[J].亚太传统医药,2017,13(1):12-14.
[10]宁海燕,谢萍,冯俭,等.参归仁合剂对产后抑郁大鼠行为学及雌激素介导下ERK1/2通路中MEK1的影响[J].中国实验方剂学杂志,2017,34(3):96-101.
[11]谢萍,侯玉敏,冯俭.参归仁合剂对产后抑郁大鼠雌激素和额叶p-CREB及p-ERK1/2的影响[J].华西药学杂志,2017,32(3):254-256.
[12]夏先锋,刘庆义,李敏,等.围绝经期抑郁小鼠模型的建立及评估[J].中华行为医学与脑科学杂志,2018,20(7):655-660.
[13]李春雨,刘梦,谢萍.补虚化瘀法对产后抑郁低雌激素大鼠海马核糖体S6激酶和丝裂原活化细胞外调节激酶1的影响[J].科学技术与工程,2018,27(7):35-38.
[14]徐雯,刘锟,林秋婷,等.糖尿病合并抑郁症动物模型及其脑内胶质原纤维酸性蛋白的表达[J].中华医学杂志,2016,96(29):2360-2364.
[15]张锦花,洪兰,吕学玉,等.低阻抗意念导入疗法对抑郁症患者外周神经递质的影响[J].国际中医中药杂志,2016,38(10):895-897.
[16]唐文娟,钱一平,顾康莹,等.联合综合心理干预对复发性抑郁症患者抑郁情绪、生命健康质量及神经递质的影响[J].中国医药导报,2017,14(34):25-28.
[17]荣晅,彭建美,宋宇,等.探讨二甲双胍对多囊卵巢综合征患者的疗效及其心理健康和血清单胺类神经递质浓度的关系[J].国际精神病学杂志,2017,30(5):867-869.
[18]呼日乐巴根,松林,乌兰其其格,等.肉蔻五味丸对慢性应激性抑郁症大鼠学习记忆能力及海马BDNF、TrkB蛋白表达的影响[J].中华中医药杂志,2017,26(8):3797-3800.