穴位埋线对围绝经期大鼠脂质代谢的影响及机制研究
|
摘要
目的:探讨穴位埋线法对围绝经期大鼠脂质代谢的影响及其机制。方法:利用双侧卵巢切除(OVX)的方法制备围绝经期大鼠模型,SD大鼠随机分为假手术组、模型组、雌激素组和埋线组,每组10只。比较各组大鼠体质量变化、子宫系数、大鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)和高密度脂蛋白(HDL)及肝脏组织中腺苷酸活化蛋白激酶(AMPKα)、磷酸化腺苷酸活化蛋白激酶(p-AMPKα)、乙酰辅酶A羧化酶(ACC)及磷酸化乙酰辅酶A羧化酶(p-ACC)蛋白表达。结果:与假手术组相比,模型组大鼠体质量明显升高、子宫系数却明显降低、血清中TC、TG、LDL含量均升高,而HDL含量下降、肝脏组织中p-AMPKα、p-ACC表达均有所下降,具有显著性差异(P<0.01)。经穴位埋线治疗后,大鼠体质量明显降低、子宫系数升高、血清中TC、TG、LDL含量均降低,而HDL含升高、肝脏组织中p-AMPKα、p-ACC表达均有所升高,具有显著性差异(P<0.05,P<0.01)。结论:穴位埋线法能够改善围绝经期大鼠脂质代谢紊乱,其可能的机制是通过激活AMPK信号通路,促进AMPKα、ACC磷酸化实现的。
Objective: To explore the effect of acupoint catgut-embedding therapy on lipid metabolism and its possible mechanism in the perimenopendal rats. Methods: SD rats were randomly divided into the sham group, the model group, the estrogen group and the acupoint catgut-embedding therapy group, 10 mice in each group. In this study, the perimenopausal model in rats was established by Ovariectomy. Compared the differences in rats weight, uterus coefficient, total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL) and high-density lipoprotein(HDL) levels and the expressions of AMPK α, p-AMPK α, ACC and p-ACC were examined by Western Blot. Results: Compared with the sham group, model group's rats weight increased significantly, uterus coefficient was lower significantly, and TC, TG and LDL levels were increased, and HDL levels were decreased. The expressions of p-AMPK α and p-ACC proteins were statistically decreasedcompared with that of the sham group(P<0.01). After acupoint catgut-embedding therapy, the weight of ratssignificantly reduced. The uterus coefficient increased. TC, TG and LDL levels were decreasedand HDL levelsincreased. The expressions of p-AMPK α and p-ACC protein were increasedwith significant difference(P<0.05, P<0.01). Conclusion: The acupoint catgut-embedding therapy improved the lipid metabolism disorder in the perimenopaual ratsand its possible mechanism is to activate AMPK signaling pathway to promote AMPK α and ACC phosphorylation.
Objective: To explore the effect of acupoint catgut-embedding therapy on lipid metabolism and its possible mechanism in the perimenopendal rats. Methods: SD rats were randomly divided into the sham group, the model group, the estrogen group and the acupoint catgut-embedding therapy group, 10 mice in each group. In this study, the perimenopausal model in rats was established by Ovariectomy. Compared the differences in rats weight, uterus coefficient, total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL) and high-density lipoprotein(HDL) levels and the expressions of AMPK α, p-AMPK α, ACC and p-ACC were examined by Western Blot. Results: Compared with the sham group, model group's rats weight increased significantly, uterus coefficient was lower significantly, and TC, TG and LDL levels were increased, and HDL levels were decreased. The expressions of p-AMPK α and p-ACC proteins were statistically decreasedcompared with that of the sham group(P<0.01). After acupoint catgut-embedding therapy, the weight of ratssignificantly reduced. The uterus coefficient increased. TC, TG and LDL levels were decreasedand HDL levelsincreased. The expressions of p-AMPK α and p-ACC protein were increasedwith significant difference(P<0.05, P<0.01). Conclusion: The acupoint catgut-embedding therapy improved the lipid metabolism disorder in the perimenopaual ratsand its possible mechanism is to activate AMPK signaling pathway to promote AMPK α and ACC phosphorylation.
引文
[1] Kirichenko TV, Myasoedova VA, Orekhova VA, et al. Phytoestrogen-Rich Natural Preparation for Treatment of Climacteric Syndrome and Atherosclerosis Prevention in Perimenopausal Women[J]. Phytother Res, 2017, 31(8):1209-1214.
[2] Olszanecka A, Kawecka-Jaszcz K, Czarnecka D. Association of free testosterone and sex hormone binding globulin with metabolic syndrome and subclinical atherosclerosis but not blood pressure in hypertensive perimenopausal women[J]. Arch Med Sci, 2016, 12(3):521-528.
[3] 厉婷, 崔燎. 脂质代谢紊乱和骨质疏松[J]. 中国骨质疏松杂志, 2008, 6:428-432.
[4] Xing X, Su L, Asare PF, et al. Danzhi Qing’e (DZQE) activates AMPK pathway and regulates lipid metabolism in a rat model of perimenopausal hyperlipidaemia[J]. Exp Physiol, 2016, 101(11):1406-1417.
[5] 庞大勇. 玫参颗粒对围绝经期模型大鼠内分泌-免疫功能和血脂代谢的研究[D].兰州:兰州大学,2015.
[6] 吴莹, 汤晓冬, 徐美君, 等. 穴位埋线对妇女肥胖和围绝经期综合征的疗效观察[J]. 针灸临床杂志, 2016, 32(8):36-42.
[7] 高磊, 孔显娟, 石现. 电针和穴位埋线对单纯性肥胖大鼠脂质代谢基因PPAR-γ mRNA表达及相关脂代谢酶的影响[J]. 中国针灸, 2011, 31(6):535-538.
[8] 李由, 徐玲, 秦卓, 等. 电针“三阴交”对自然围绝经期大鼠下丘脑-垂体-卵巢轴的影响[J]. 针刺研究, 2014, 39(3):198-201.
[9] Garcia-Vivas JM, Galaviz-Hernandez C, Fernandez-Retana J, et al.Transcriptomic Profiling of Adipose Tissue in Obese Women in Response to Acupuncture Catgut Embedding Therapy with Moxibustion[J].J Altern Complement Med, 2016, 22(8):658-668.
[10] Liu P, Ji Y, Yuen T, et al. Blocking FSH induces thermogenic adipose tissue and reduces body fat[J]. Nature, 2017, 546(7656):107-112.
[11] 蒋希荣, 任路, 李春日. 电针对围绝经期抑郁症大鼠下丘脑-垂体-卵巢轴的影响[J]. 针刺研究, 2017, 42(1):45-49.
[12] 何秋明, 肖尚杰, 夏慧敏. 大鼠阴道涂片的观察[J]. 广州医学院学报, 2007, 35(4):54-56.
[13] 李忠仁.实验针灸学[M]. 北京:中国中医药出版社, 2003:147-148.
[14] Gemma Hammerton, Gordon Harold, Anita Thapar,et al. Depression and blood pressure in high-risk children and adolescents: an investigation using two longitudinal cohorts[J]. BMJ Open, 2013, 3(9):e003206.
[15] Chen YY, Ma LX, Zhu J. Advances of studies on acupuncture and moxibustion for perimenopausal syndrome[J]. Zhongguo Zhen Jiu, 2010, 30(3):257-260.
[16] Woo YS, Seo HJ, McIntyre RS, et al. Obesity and Its Potential Effects on Antidepressant Treatment Outcomes in Patients with Depressive Disorders: A Literature Review[J]. Int J Mol Sci, 2016, 17(1):80.
[17] 于琦, 陈霞. 围绝经期综合征的中医药治疗进展[J]. 辽宁中医药大学学报, 2009, 11(2): 47.
[18] 王海荣,尹丽丽,李春梅,等.穴位埋线治疗肥胖症临床及机理研究进展[J].陕西中医,2011,32(3):374-376.
[19] 刘延明, 苏和平. 穴位埋线治疗单纯性肥胖症临床疗效观察[J]. 辽宁中医杂志, 2008, 35(4): 599-600.
[20] 郭爱松. 穴位埋线疗法研究近况[J].针灸临床杂志, 2013(7): 89-91.
[21] Nisoli E, Carruba MO. Nitric oxide and mitochondrial biogenesis[J]. J Cell Sci, 2006, 19 (Pt 14):2855-2862.
[22] O’Neill HM, Holloway GP, Steinberg GR. AMPK regulation of fatty acid metabolism and mitochondrial biogenesis: Implications for obesity[J]. Mol Cell Endocrinol, 2013, 366(2): 135-151.
[23] Zhang Y, Yang X, Shi H, et al. Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes[J]. Lipids Health Dis, 2011, 10:122.
[24] Lu J, Huang G, Hu S, et al. 1,3-Dichloro-2-propanol induced hyperlipidemia in C57BL/6J mice via AMPK signaling pathway[J]. Food Chem Toxicol, 2014, 64:403-409.
[25] Ou H, Liu C, Feng W, et al. Role of AMPK in atherosclerosis via autophagy regulation[J]. Sci China Life Sci, 2018, 61(10): 1212-1221.
[26] Coletta DK, Sriwijitkamol A, Wajcberg E, et al. Pioglitazone stimulates AMP-activated protein kinase signalling and increases the expression of genes involved in adiponectin signalling, mitochondrial function and fat oxidation in human skeletal muscle in vivo: a randomised trial[J]. Diabetologia, 2009, 52(4):723-732.
[2] Olszanecka A, Kawecka-Jaszcz K, Czarnecka D. Association of free testosterone and sex hormone binding globulin with metabolic syndrome and subclinical atherosclerosis but not blood pressure in hypertensive perimenopausal women[J]. Arch Med Sci, 2016, 12(3):521-528.
[3] 厉婷, 崔燎. 脂质代谢紊乱和骨质疏松[J]. 中国骨质疏松杂志, 2008, 6:428-432.
[4] Xing X, Su L, Asare PF, et al. Danzhi Qing’e (DZQE) activates AMPK pathway and regulates lipid metabolism in a rat model of perimenopausal hyperlipidaemia[J]. Exp Physiol, 2016, 101(11):1406-1417.
[5] 庞大勇. 玫参颗粒对围绝经期模型大鼠内分泌-免疫功能和血脂代谢的研究[D].兰州:兰州大学,2015.
[6] 吴莹, 汤晓冬, 徐美君, 等. 穴位埋线对妇女肥胖和围绝经期综合征的疗效观察[J]. 针灸临床杂志, 2016, 32(8):36-42.
[7] 高磊, 孔显娟, 石现. 电针和穴位埋线对单纯性肥胖大鼠脂质代谢基因PPAR-γ mRNA表达及相关脂代谢酶的影响[J]. 中国针灸, 2011, 31(6):535-538.
[8] 李由, 徐玲, 秦卓, 等. 电针“三阴交”对自然围绝经期大鼠下丘脑-垂体-卵巢轴的影响[J]. 针刺研究, 2014, 39(3):198-201.
[9] Garcia-Vivas JM, Galaviz-Hernandez C, Fernandez-Retana J, et al.Transcriptomic Profiling of Adipose Tissue in Obese Women in Response to Acupuncture Catgut Embedding Therapy with Moxibustion[J].J Altern Complement Med, 2016, 22(8):658-668.
[10] Liu P, Ji Y, Yuen T, et al. Blocking FSH induces thermogenic adipose tissue and reduces body fat[J]. Nature, 2017, 546(7656):107-112.
[11] 蒋希荣, 任路, 李春日. 电针对围绝经期抑郁症大鼠下丘脑-垂体-卵巢轴的影响[J]. 针刺研究, 2017, 42(1):45-49.
[12] 何秋明, 肖尚杰, 夏慧敏. 大鼠阴道涂片的观察[J]. 广州医学院学报, 2007, 35(4):54-56.
[13] 李忠仁.实验针灸学[M]. 北京:中国中医药出版社, 2003:147-148.
[14] Gemma Hammerton, Gordon Harold, Anita Thapar,et al. Depression and blood pressure in high-risk children and adolescents: an investigation using two longitudinal cohorts[J]. BMJ Open, 2013, 3(9):e003206.
[15] Chen YY, Ma LX, Zhu J. Advances of studies on acupuncture and moxibustion for perimenopausal syndrome[J]. Zhongguo Zhen Jiu, 2010, 30(3):257-260.
[16] Woo YS, Seo HJ, McIntyre RS, et al. Obesity and Its Potential Effects on Antidepressant Treatment Outcomes in Patients with Depressive Disorders: A Literature Review[J]. Int J Mol Sci, 2016, 17(1):80.
[17] 于琦, 陈霞. 围绝经期综合征的中医药治疗进展[J]. 辽宁中医药大学学报, 2009, 11(2): 47.
[18] 王海荣,尹丽丽,李春梅,等.穴位埋线治疗肥胖症临床及机理研究进展[J].陕西中医,2011,32(3):374-376.
[19] 刘延明, 苏和平. 穴位埋线治疗单纯性肥胖症临床疗效观察[J]. 辽宁中医杂志, 2008, 35(4): 599-600.
[20] 郭爱松. 穴位埋线疗法研究近况[J].针灸临床杂志, 2013(7): 89-91.
[21] Nisoli E, Carruba MO. Nitric oxide and mitochondrial biogenesis[J]. J Cell Sci, 2006, 19 (Pt 14):2855-2862.
[22] O’Neill HM, Holloway GP, Steinberg GR. AMPK regulation of fatty acid metabolism and mitochondrial biogenesis: Implications for obesity[J]. Mol Cell Endocrinol, 2013, 366(2): 135-151.
[23] Zhang Y, Yang X, Shi H, et al. Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes[J]. Lipids Health Dis, 2011, 10:122.
[24] Lu J, Huang G, Hu S, et al. 1,3-Dichloro-2-propanol induced hyperlipidemia in C57BL/6J mice via AMPK signaling pathway[J]. Food Chem Toxicol, 2014, 64:403-409.
[25] Ou H, Liu C, Feng W, et al. Role of AMPK in atherosclerosis via autophagy regulation[J]. Sci China Life Sci, 2018, 61(10): 1212-1221.
[26] Coletta DK, Sriwijitkamol A, Wajcberg E, et al. Pioglitazone stimulates AMP-activated protein kinase signalling and increases the expression of genes involved in adiponectin signalling, mitochondrial function and fat oxidation in human skeletal muscle in vivo: a randomised trial[J]. Diabetologia, 2009, 52(4):723-732.