质膜H~+-ATPase对2种湿地植物吸收NO_3~-的影响
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摘要
为研究湿地植物净化硝态氮(NO_3~-)的效果及吸收机理,以风眼莲、美人蕉2种常用湿地植物为实验材料,采用溶液培养法研究2种植物在模拟农田水体中对NO_3~-的去除率与质膜H~+-ATPase活性及其与14-3-3蛋白的互作水平的关系。结果表明:凤眼莲对NO_3~-的去除率高于美人蕉,其根尖质膜H~+-ATPase活性、叶片中硝酸还原酶活性均大于美人蕉。质膜H~+-ATPase活性影响植株NO_3~-的吸收能力,表明质膜H~+-ATPase参与了植物吸收NO_3~-的调控作用。
The aim of the experment was to study the absorption mechanism of nitrate nitrogen( NO_3~-) in two species of wetland plants. A hydroponic experiment was conducted with Eichhornia Crassipes and Cana generalis Bailey to study plasma membrane H~+-ATPase( PM H~+-ATPase) activity and their interaction with 14-3-3 protein on NO_3~-removal rate of two species of wetland plants in simulated farmland water. The results showed that the removal rate of NO_3~-and PM H~+-ATPase activity in Eichhornia Crassipes was higher than those in Cana generalis Bailey,and activity of nitrate reductase in leaves was also higher than that of Cana generalis Bailey. The study revealed that higher activity of PM H~+-ATPase could enhance sequentially absorption of NO_3~-,which indicated that PM H~+-ATPase was involved in regulation of NO_3~-uptake in plants.
The aim of the experment was to study the absorption mechanism of nitrate nitrogen( NO_3~-) in two species of wetland plants. A hydroponic experiment was conducted with Eichhornia Crassipes and Cana generalis Bailey to study plasma membrane H~+-ATPase( PM H~+-ATPase) activity and their interaction with 14-3-3 protein on NO_3~-removal rate of two species of wetland plants in simulated farmland water. The results showed that the removal rate of NO_3~-and PM H~+-ATPase activity in Eichhornia Crassipes was higher than those in Cana generalis Bailey,and activity of nitrate reductase in leaves was also higher than that of Cana generalis Bailey. The study revealed that higher activity of PM H~+-ATPase could enhance sequentially absorption of NO_3~-,which indicated that PM H~+-ATPase was involved in regulation of NO_3~-uptake in plants.
引文
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[19]Rengel Z,Bose J,Chen Q,et al.Magnesium alleviates plant toxicity of aluminium and heavy metals[J].Crop and Pasture Science,2015,66(12):1298-1307.
[20]Kerkeb L,Venema K,Donaire J P,et al.Enhanced H+-ATPcoupling ratio of H+-ATPase and increased 14-3-3 protein content in plasma membrane of tomato cells upon osmotic shock[J].Physiologia Plantarum,2002,116(1):37-41.
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[22]Falhof J,Pedersen J T,Fuglsang A T,et al.Plasma membrane H+-ATPase regulation in the center of plant physiology[J].Molecular Plant,2016,9(3):323-337.
[23]Mc Clure P R,Kochian L V,Spanswick R M,et al.Evidence for cotransport of nitrate and protons in maize rootsⅡ.Measurement of NO-3and H+fluxes with ion-selective microelectrodes[J].Plant Physiology,1990,93(1):290-294.
[24]Alterfjord M,Sehnke P C,Arkell A.Plasma membrane H+-ATPase and 14-3-3 isoforms of Arabidopsis leaves:evidence for isoform specicity in the 14-3-3/H+-ATPase interaction[J].Plant Cell Physiol,2004,45(9):1202-1210.
[25]周小华,徐慧妮,谷照虎,等.铝胁迫下质膜H+-ATPase对水稻硝态氮吸收的影响[J].植物生理学报,2016,52(7):1011-1018.
[26]陈东杰,王平,庞晓璐,等.铝胁迫下不同耐铝性黑大豆硝态氮吸收机理研究[J].大豆科学,2014,33(6):870-875.
[2]郝明旭,霍莉莉,吴珊珊.人工湿地植物水体净化效能研究进展[J].环境工程,2017,35(8):5-10.
[3]Yan S H,Song W,Guo J Y.Advances in management and utilizationof invasive water hyacinth(Eichhorniacrassipes)in a aquatic ecosystems:a review[J].Critical Reviews in Biotechnology,2016,37(2):1-11.
[4]闻学政,刘海琴,张迎颖,等.凤眼莲和水浮莲对滇池草海水体中氮去除效果的比较研究[J].农业资源与环境学报,2015,32(4):388-394.
[5]张志勇,徐寸发,严少华,等.凤眼莲生态修复工程改善滇池水质及湖体氮磷收支平衡[J].农业工程学报,2017,33(13):235-242.
[6]张亚娟,王军霞,刘存歧,等.美人蕉浮床对富营养水体氮磷去除效果的研究进展[J].安徽农业科学,2011,39(10):6053-6054.
[7]吴诗杰,陈慧娟,许小桃,等.美人蕉、鸢尾、黄菖蒲和千屈菜对富营养化水体净化效果研究[J].安徽大学学报(自然科学版),2016,40(1):98-108.
[8]国家环境保护总局,国家质量监督检验检疫总局.地表水环境质量标准:GB 3838-2002[S].北京:中国科学出版社,2002.
[9]国家环境保护总局.中华人民共和国环境保护行业标准:HJ/T346-2007[S].北京:中国环境科学出版社,2007:1-2.
[10]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:119-120.
[11]Shen H,He L F,Sasaki T,et al.Citrate secretion coupled with the modulation of soybean root tip under aluminum stress.Upregulation of transcription,translation,and threonine-oriented phosphorylation of plasma membrane H+-ATPase[J].Plant Physiology,2005,138(1):287-296.
[12]Guo C L,Chen Q,Zhao X L,et al.Al-enhanced expression and interaction of 14-3-3 protein and plasma membrane H+-ATPase is related to Al-induced citrate secretion in an Al-resistant black soybean[J].Plant Molecular Biology Reporter,2013,31(4):1012-1024.
[13]Zhou X H,Gu Z H,Xu H N et al.The effects of exogenous ascorbic acid on the mechanism of physiological and biochemical responses to nitrate uptake in two rice cultivars(Oryza sativa L.)under aluminum stress[J].Journal of Plant Growth Regulation,2016,35(4):1013-1024.
[14]Zhang R P,Liu G,Wu N,et al.Adaptation of plasma membrane H+-ATPase and H+-pump to P deciency in rice roots[J].Plant and Soil,2011,349(1/2):3-11.
[15]Elmore J M,Coaker G.The role of the plasma membrane H+-ATPase in plant-microbe interactions[J].Molecular Plant,2011,4(3):416-427.
[16]曹优明.美人蕉人工湿地对城市生活污水的净化研究[J].环境科学技术,2009,32(7):120-124.
[17]刘立岩,吕兴娜.用凤眼莲净化水质中氮、磷污染物的研究分析[J].丹东纺专学报,2005,12(2):40-41.
[18]Morsomme P,Boutry M.The plant plasma membrane H+-ATPase:strcture,function and regulation[J].Biochim Biophysica Acta,2000,1465(1/2):1-16.
[19]Rengel Z,Bose J,Chen Q,et al.Magnesium alleviates plant toxicity of aluminium and heavy metals[J].Crop and Pasture Science,2015,66(12):1298-1307.
[20]Kerkeb L,Venema K,Donaire J P,et al.Enhanced H+-ATPcoupling ratio of H+-ATPase and increased 14-3-3 protein content in plasma membrane of tomato cells upon osmotic shock[J].Physiologia Plantarum,2002,116(1):37-41.
[21]Sondergaard T E,Schuijz A,Palmgren M G.Energization of transport processes in plants.roles of the plasma membrane H+-ATPase[J].Plant Physiology,2004,136(1):2475-2482.
[22]Falhof J,Pedersen J T,Fuglsang A T,et al.Plasma membrane H+-ATPase regulation in the center of plant physiology[J].Molecular Plant,2016,9(3):323-337.
[23]Mc Clure P R,Kochian L V,Spanswick R M,et al.Evidence for cotransport of nitrate and protons in maize rootsⅡ.Measurement of NO-3and H+fluxes with ion-selective microelectrodes[J].Plant Physiology,1990,93(1):290-294.
[24]Alterfjord M,Sehnke P C,Arkell A.Plasma membrane H+-ATPase and 14-3-3 isoforms of Arabidopsis leaves:evidence for isoform specicity in the 14-3-3/H+-ATPase interaction[J].Plant Cell Physiol,2004,45(9):1202-1210.
[25]周小华,徐慧妮,谷照虎,等.铝胁迫下质膜H+-ATPase对水稻硝态氮吸收的影响[J].植物生理学报,2016,52(7):1011-1018.
[26]陈东杰,王平,庞晓璐,等.铝胁迫下不同耐铝性黑大豆硝态氮吸收机理研究[J].大豆科学,2014,33(6):870-875.