扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠微小RNA-29家族表达的影响
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摘要
目的通过观察扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠微小RNA-29家族(microRNA-29,miR-29)的表达影响,探讨其抗心肌纤维化的可能作用机制。方法采用冠状动脉左前降支结扎术制备大鼠急性心肌梗死模型,随机分为模型组、扶正化瘀组和卡托普利组,假手术组只穿线不结扎,每组6只,连续8周灌胃给予相应药物或去离子水。采用HE染色、心脏指数评分、Masson染色的方法评价心脏整体形态结构、重构程度及纤维化程度,并运用实时荧光定量PCR法检测miR-29家族表达水平。结果 (1)与假手术组相比,模型组大鼠心脏指数比值、胶原容积分数升高(P<0.01);与模型组相比,扶正化瘀组、卡托普利组大鼠心脏指数比值、胶原容积分数均降低(P<0.01)。(2)与假手术组相比,模型组大鼠心肌梗死边缘区组织中miR-29a-3p、miR-29b-3p、miR-29c-3p、miR-29b-5p、miR-29c-5p表达显著下调(P<0.05)。与模型组比较,扶正化瘀组miR-29b-3p、miR-29a-5p、miR-29b-5p、miR-29c-5p表达上调(P<0.05);卡托普利组miR-29a-5p、miR-29b-5p、miR-29c-5p表达水平显著升高(P<0.01),miR-29a-3p、miR-29b-3p、miR-29c-3p表达水平进一步下调(P<0.01)。结论扶正化瘀胶囊可改善大鼠心肌梗死后心肌纤维化,其作用机制可能与上调部分miR-29家族成员的表达有关。
Objective To investigate the effect of Fuzheng Huayu Capsule on the expression of miR-29 family in rats with myocardial fibrosis after myocardial infarction, and to explore the possible mechanism of Fuzheng Huayu Capsule on myocardial fibrosis. Methods The model of acute myocardial infarction was established by ligation of the left anterior descending coronary artery in each rat. The rats were randomly divided into model group, Fuzheng Huayu group and captopril group. The sham operation group was only threaded without ligation of the left anterior descending coronary artery. There were 6 rats in each group. The corresponding drugs or deionized water were given by gavage for 8 weeks. The cardiac morphology and the degree of remodeling and myocardial fibrosis were evaluated by HE staining, cardiac index ratio and Masson staining. The expression of miR-29 family was detected by real-time fluorescence quantitative PCR. Results(1) Compared with the sham operation group, the cardiac index ratio and collagen volume fraction in the model group increased(P<0.01). Compared with the model group, the cardiac index ratio and collagen volume fraction in the Fuzheng Huayu group and captopril group were decreased(P<0.01).(2) Compared with the sham operation group, the expressions of miR-29 a-3 p, miR-29 b-3 p, miR-29 c-3 p, miR-29 b-5 p and miR-29 c-5 p in the model group decreased(P<0.05). While compared with the model group, the expressions of miR-29 b-3 p, miR-29 a-5 p, miR-29 b-5 p and miR-29 c-5 p were increased in the Fuzheng Huayu group(P<0.05); the expression levels of miR-29 a-5 p, miR-29 b-5 p and miR-29 c-5 p were significantly increased in the captopril group(P<0.01), and the expression levels of miR-29 a-3 p, miR-29 b-3 p and miR-29 c-3 p were further down-regulated(P<0. 01).Conclusion Fuzheng Huayu Capsule can improve myocardial fibrosis after myocardial infarction in rats, and the mechanism may be related to up-regulating the expression of part of miR-29 family.
Objective To investigate the effect of Fuzheng Huayu Capsule on the expression of miR-29 family in rats with myocardial fibrosis after myocardial infarction, and to explore the possible mechanism of Fuzheng Huayu Capsule on myocardial fibrosis. Methods The model of acute myocardial infarction was established by ligation of the left anterior descending coronary artery in each rat. The rats were randomly divided into model group, Fuzheng Huayu group and captopril group. The sham operation group was only threaded without ligation of the left anterior descending coronary artery. There were 6 rats in each group. The corresponding drugs or deionized water were given by gavage for 8 weeks. The cardiac morphology and the degree of remodeling and myocardial fibrosis were evaluated by HE staining, cardiac index ratio and Masson staining. The expression of miR-29 family was detected by real-time fluorescence quantitative PCR. Results(1) Compared with the sham operation group, the cardiac index ratio and collagen volume fraction in the model group increased(P<0.01). Compared with the model group, the cardiac index ratio and collagen volume fraction in the Fuzheng Huayu group and captopril group were decreased(P<0.01).(2) Compared with the sham operation group, the expressions of miR-29 a-3 p, miR-29 b-3 p, miR-29 c-3 p, miR-29 b-5 p and miR-29 c-5 p in the model group decreased(P<0.05). While compared with the model group, the expressions of miR-29 b-3 p, miR-29 a-5 p, miR-29 b-5 p and miR-29 c-5 p were increased in the Fuzheng Huayu group(P<0.05); the expression levels of miR-29 a-5 p, miR-29 b-5 p and miR-29 c-5 p were significantly increased in the captopril group(P<0.01), and the expression levels of miR-29 a-3 p, miR-29 b-3 p and miR-29 c-3 p were further down-regulated(P<0. 01).Conclusion Fuzheng Huayu Capsule can improve myocardial fibrosis after myocardial infarction in rats, and the mechanism may be related to up-regulating the expression of part of miR-29 family.
引文
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[12] 祁轶斐,任学娇,娄利霞,等.扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠细胞外基质代谢的影响[J].中医杂志,2018,59(7):607-611.
[13] 任学娇,祁轶斐,娄利霞,等.扶正化瘀胶囊对心肌梗死大鼠心肌纤维化及TGF-β_1/Smads信号通路的影响[J].中西医结合心脑血管病杂志,2018,16(9):1185-1189.
[14] YE Y,HU Z,LIN Y,et al.Downregulation of microRNA-29 by antisense inhibitors and a PPAR-g agonist protects against myocardial ischaemia– reperfusion injury[J].Cardiovasc Res 2010,87:535-544.
[15] RODERBURG C,URBAN G W,BETTERMANN K ,et al.Micro-RNA profiling reveals a role for miR-29 in human and murine liver fibrosis[J].Hepatology,2011,53(1):209-218.
[16] CUSHING L,KUANG P P,QIAN J,et al.miR-29 Is a Major Regulator of Genes Associated with Pulmonary Fibrosis[J].American Journal of Respiratory Cell and Molecular Biology,2011,45(2):287-294.
[2] 胡晨,张瑶.非编码RNA在心肌纤维化中作用研究的进展[J].心血管康复医学杂志,2017,26(6):676-678.
[3] LEWIS B P,BURGE C B,BARTEL D P .Conserved Seed Pairing,Often Flanked by Adenosines,Indicates that Thousands of Human Genes are MicroRNA Targets[J].Cell,2005,120(1):1-20.
[4] BARTEL D P .MicroRNAs:genomics,biogenesis,mechanism,and function.[J].Cell,2004,116(2):281-297.
[5] ZHANG Y,HUANG X R,WEI L H,et al.miR-29b as a therapeutic agent for angiotensin II-induced cardiac fibrosis by targeting TGF-β/Smad3 signaling[J].Mol Ther,2014,22(5):974-985.
[6] PATEL V,NOUREDDINE L.MicroRNAs and fibrosis[J].Curr Opin Nephrol Hypertens,2012,21(4):410-416.
[7] RONCARATI R ,ANSELMI C V,LOSI M A ,et al.Circulating miR-29a,among other up-regulated microRNAs,is the only biomarker for both hypertrophy and fibrosis in patients with hypertrophic cardiomyopathy[J].Journal of the American College of Cardiology,2014,63(9):920-927.
[8] ROOIJ E V ,SUTHERLAND L B ,THATCHER J E ,et al.Dysregulation of microRNAs after myocardial infarction reveals a role of miR-29 in cardiac fibrosis[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(35):13027-13032.
[9] ZHU H ,FAN G C .Role of microRNAs in the reperfused myocardium towards post-infarct remodeling [J].Cardiovascular Research,2012,94(2):284-292.
[10] WANG B,Komers R,Carew R,et al.Suppression of microRNA-29 expression by TGF-beta1 promotes collagen expression and renal fibrosis [J].J Am Soc Nephrol,2012,23(2):252-265.
[11] 吴爱明,张冬梅,娄利霞,等.扶正化瘀胶囊对心肌梗死大鼠心脏结构和功能的影响[J].中西医结合心脑血管病杂志,2013,11(11):1347-1349.
[12] 祁轶斐,任学娇,娄利霞,等.扶正化瘀胶囊对心肌梗死后心肌纤维化大鼠细胞外基质代谢的影响[J].中医杂志,2018,59(7):607-611.
[13] 任学娇,祁轶斐,娄利霞,等.扶正化瘀胶囊对心肌梗死大鼠心肌纤维化及TGF-β_1/Smads信号通路的影响[J].中西医结合心脑血管病杂志,2018,16(9):1185-1189.
[14] YE Y,HU Z,LIN Y,et al.Downregulation of microRNA-29 by antisense inhibitors and a PPAR-g agonist protects against myocardial ischaemia– reperfusion injury[J].Cardiovasc Res 2010,87:535-544.
[15] RODERBURG C,URBAN G W,BETTERMANN K ,et al.Micro-RNA profiling reveals a role for miR-29 in human and murine liver fibrosis[J].Hepatology,2011,53(1):209-218.
[16] CUSHING L,KUANG P P,QIAN J,et al.miR-29 Is a Major Regulator of Genes Associated with Pulmonary Fibrosis[J].American Journal of Respiratory Cell and Molecular Biology,2011,45(2):287-294.