根皮苷对油酸诱导HepG2细胞脂肪及胆固醇代谢基因表达的影响
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摘要
以噻唑兰(MTT)比色法检测细胞活力,筛选出合适的根皮苷药物浓度及油酸浓度,建立油酸诱导肝癌细胞(Hep G2)产生脂肪堆积体外模型。分别以MTT值、Hep G2细胞形态、Oil Red O脂滴染色、甘油三酯(TG值)、胆固醇(TC值)、脂肪代谢及胆固醇代谢基因SREBP-1C、FAS、CPT-1、PPARα、HMGCR mRNA表达水平为指标,考察根皮苷对油酸高脂模型细胞的影响。根皮苷能够显著抑制油酸诱导的Hep G2细胞脂肪堆积作用,90μmol/L根皮苷能够降低脂肪合成相关基因的表达,提高脂肪氧化代谢基因的表达,降低胆固醇合成相关基因的表达。
Cell viability was determined by MTT assay, through which the appropriate phloridzin concentrations could be chosen and the oleic acid-induced lipid accumulation model in HepG2 cell in vitro could be established. MTT value, HepG2 cell morphology, Oil Red O staining, total triglyceride(TG), total cholesterol(TC), lipid and cholesterol metabolism genes such as SREBP-1 C、FAS、CPT-1、 PPARα、HMGCR mRNA expression level were used to evaluate the effect of phloridzin on HepG2 steatosis. Phloridzin showed a significant inhibitory effect against TG accumulation in HepG2 cells induced by Oleic acid. 90 μmol/L phloridzin could decrease the genes expression related to lipid synthesis on the contrary, increase the genes expression related to lipid oxidation metabolism, furthermore, decrease the genes expression related to cholesterol synthesis.
Cell viability was determined by MTT assay, through which the appropriate phloridzin concentrations could be chosen and the oleic acid-induced lipid accumulation model in HepG2 cell in vitro could be established. MTT value, HepG2 cell morphology, Oil Red O staining, total triglyceride(TG), total cholesterol(TC), lipid and cholesterol metabolism genes such as SREBP-1 C、FAS、CPT-1、 PPARα、HMGCR mRNA expression level were used to evaluate the effect of phloridzin on HepG2 steatosis. Phloridzin showed a significant inhibitory effect against TG accumulation in HepG2 cells induced by Oleic acid. 90 μmol/L phloridzin could decrease the genes expression related to lipid synthesis on the contrary, increase the genes expression related to lipid oxidation metabolism, furthermore, decrease the genes expression related to cholesterol synthesis.
引文
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[2]申婷婷,花尔并,马娜,等.根皮苷对饲喂高脂高胆固醇饲料仓鼠肝脏中胆固醇代谢调控基因表达的影响[J].营养学报,2014,36(2):159-163.
[3]YUAN X,WU Y,LIANG J,et al.Phlorizin treatment attenuates obesity and related disorders through improving BAT thermogenesis[J].Journal of Functional Foods,2016,27:429-438.
[4]PEI F,LI B Y,ZHANG Z,et al.Beneficial effects of phlorizin on diabetic nephropathy in diabetic db/db mice[J].Journal of Diabetes&Its Complications,2014,28(5):596-603.
[5]KONG X,GAO Y,GENG X,et al.Effect of lipid lowering tablet on blood lipid in hyperlipidemia model rats[J].Saudi Journal of Biological Sciences,2017,25(4):715-718.
[6]申婷婷,马娜,梁若男,等.迷迭香对仓鼠肝脏胆固醇代谢调控基因表达的影响[J].中国食品学报,2015,15(3):8-14.
[7]SHAO D,WANG Y,HUANG Q,et al.Cholesterol-Lowering effects and mechanisms in view of bile acid pathway of resveratrol and resveratrol glucuronides[J].Journal of Food Science,2016,81(11):2841-2848.
[8]吕业春,刘翼翔,吴薇,等.蓝莓多酚对油酸诱导Hep G2细胞脂肪累积的干预作用[J].食品科学,2011,32(17):308-312.
[9]杨胜楠,李祥,王慧,等.木浆源甾醇对高脂高胆固醇仓鼠胆固醇代谢基因表达的影响[J].营养学报,2018,40(1):53-58.
[10]LYONS J G,O DEA K,WALKER K Z.Evidence for low highdensity lipoprotein cholesterol levels in Australian indigenous peoples:A systematic review[J].BMC Public Health,2014,14(1):545-550.
[11]王丽华,申婷婷,张泽生,等.迷迭香提取物对高脂高胆固醇膳食仓鼠小肠中胆固醇代谢调控机制的研究[J].营养学报,2015,37(1):73-77.