金钱草组织培养及其快速繁殖研究
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摘要
[目的]建立金钱草茎段组织培养再生体系。[方法]于4、5月取当年生金钱草茎段为外植体,MS作为基础培养基,研究不同植物生长调节剂对诱导、增殖、生根过程的影响。[结果]MS+6-BA 1.0 mg/L+IBA 0.20～0.50 mg/L适合金钱草不定芽诱导,诱导率为100%;MS+6-BA1.0 mg/L+IBA 0.10～0.20 mg/L适合继代增殖培养; 1/2MS+IBA0.50 mg/L+NAA0.1～0.5 mg/L适合不定根诱导,诱导率高达100%;组培苗移植到盛有腐殖质花盆中,成活率为100%。[结论]该研究可为金钱草野生种质资源保存和优质种苗生产提供技术参考。
[Objective]To establish the tissue culture regeneration system of Lysimachia christinae Hance.stem.[Method]In April and May,the stems of L. christinae were used as explants and MS as basic medium to study the effects of different plant growth regulators on induction,proliferation and rooting. [Result]MS+6-BA 1.0 mg/L+IBA 0.20-0.50 mg/L was suitable for adventitious bud induction of L. christinae,the induction rate was 100%. MS+6-BA 1.0 mg/L+IBA0.10-0.20 mg/L was suitable for seedling subculture. 1/2MS+IBA0.50 mg/L+NAA 0.1-0.5 mg/L was suitable for root induction culture,the induction rate was 100%. The tissue culture seedlings were transplanted to pots in flowerpot with humus and the survival rate was 100%. [Conclusion]The study can provide technical reference for the conservation of wild germplasm resources and the production of high-quality seedlings of L. christinae.
[Objective]To establish the tissue culture regeneration system of Lysimachia christinae Hance.stem.[Method]In April and May,the stems of L. christinae were used as explants and MS as basic medium to study the effects of different plant growth regulators on induction,proliferation and rooting. [Result]MS+6-BA 1.0 mg/L+IBA 0.20-0.50 mg/L was suitable for adventitious bud induction of L. christinae,the induction rate was 100%. MS+6-BA 1.0 mg/L+IBA0.10-0.20 mg/L was suitable for seedling subculture. 1/2MS+IBA0.50 mg/L+NAA 0.1-0.5 mg/L was suitable for root induction culture,the induction rate was 100%. The tissue culture seedlings were transplanted to pots in flowerpot with humus and the survival rate was 100%. [Conclusion]The study can provide technical reference for the conservation of wild germplasm resources and the production of high-quality seedlings of L. christinae.
引文
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[4]李静,胡国全,桂敬强,等.金钱草水提液对小鼠肾脏草酸钙结石形成的干预作用[J].安徽科技学院学报,2014,28(2):5-11.
[5]李惠芝,姚崇舜,陈济民,等.广金钱草粗提物中多糖的含量测定[J].沈阳药科大学学报,1995,12(1):21-24.
[6]李静,贺绍君,刘德义.金钱草防治泌尿系统结石机理研究进展[J].辽宁中医药大学学报,2015,17(3):79-81.
[7]余伯阳.中药生物技术[M].北京:中国医药科技出版社,2005:195-196.
[8]梁定仁,黄明勋,林伟,等.植物生长调节剂对广金钱草扦插繁殖效果的影响[J].南方农业学报,2016,47(4):614-617.
[2]江苏新医学院.中药大词典(上册)[M].上海:上海科学技术出版社,1997:150-151.
[3]中国科学院西北植物研究所.秦岭植物志:第1卷第4册[M].北京:科学出版社,1983:29-54.
[4]李静,胡国全,桂敬强,等.金钱草水提液对小鼠肾脏草酸钙结石形成的干预作用[J].安徽科技学院学报,2014,28(2):5-11.
[5]李惠芝,姚崇舜,陈济民,等.广金钱草粗提物中多糖的含量测定[J].沈阳药科大学学报,1995,12(1):21-24.
[6]李静,贺绍君,刘德义.金钱草防治泌尿系统结石机理研究进展[J].辽宁中医药大学学报,2015,17(3):79-81.
[7]余伯阳.中药生物技术[M].北京:中国医药科技出版社,2005:195-196.
[8]梁定仁,黄明勋,林伟,等.植物生长调节剂对广金钱草扦插繁殖效果的影响[J].南方农业学报,2016,47(4):614-617.