双孢蘑菇工厂化栽培中四种木霉病害的精准鉴定
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摘要
精准鉴定是有效预防和控制双孢蘑菇工厂化栽培中病虫害的重要前提。利用病原菌形态特征和内转录间隔区(ITS)序列对双孢蘑菇工厂化栽培中的四种木霉病害进行鉴定,结果证明它们分别为:绿木霉Trichoderma virens、哈茨木霉T.harzianum、子座木霉T.stromaticum和钩状木霉T.hamatum,为今后精准鉴定双孢蘑菇工厂化栽培中的病害提供方法,同时也为双孢蘑菇工厂化栽培中这四种木霉病害的鉴定提供参照依据。
引文
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[2]ZHANG YQ,GENG W,SHEN YQ,et al.Edible mushroom cultivation for food security and rural development in China:bio-innovation,technological dissemination and marketing[J].Sustainability,2014,6(5):2961-2973.
[3]黄建春.中国双孢蘑菇工厂化生产现状与发展的思考[J].食用菌,2012,34(2):1-3.
[4]钟孟义.论制约我国双孢蘑菇工厂化生产发展的因素与对策[J].食药用菌,2013,21(3):133-138.
[5]王春宝.中国双孢蘑菇工厂化栽培的现状及技术难点的破解[J].食药用菌,2015,23(6):337-339.
[6]刘建凤,吉春明,张春梅,等.双孢蘑菇工厂化栽培主要病虫害及其防治对策[J].金陵科技学院学报,2014,30(4):79-82.
[7]GHISALBERTI E L.Anti-infective agents produced by the Hyphomycetes genera Trichoderma and Gliocladium[J].Current Medicinal Chemistry-Anti-Infective Agents,2002(1):343-374.
[8]宋金俤,曲绍轩,马林.食用菌病虫识别与防治原色图谱[M].北京:中国农业出版社,2013:1-154.
[9]边银丙.食用菌病害鉴别与防控[M].郑州:中原农民出版社,2016:1-184.
[10]BISSETT J.A revision of the genus Trichoderma.Ⅰ.Sect.Longibrachiatum sect.nov[J].Canadian Journal of Botany,1984(62):924-931.
[11]BISSETT J.A revision of the genus Trichoderma.Ⅱ.Infragenericclassification[J].CanadianJournalofBotany,1991(69):2357-2372.
[12]BISSETT J.A revision of the genus Trichoderma.Ⅲ.Sect.Pachybasium[J].Canadian Journal of Botany,1991(69):2373-2417.
[13]BISSETT J.A revision of the genus Trichoderma.Ⅳ.Additional notes on section longibrachiatum[J].Canadian Journal of Botany,1991(69):2418-2420.
[14]CHAVERRI P,SAMUELS G J.Hypocrea/Trichoderma.Ascomycota,Hypocreales,Hypocreaceae:species with green ascospores[J].Study in Mycology,2003(48):1-116.
[15]DRUZHININA I S,KOPCHINSKIY A G,KOMO M,et al.An ologonucleotide barcode for species identification in Trichoderma and Hypocrea[J].Fungal Genetics and Biology,2005,42(10):813-828.
[16]杨合同.木霉分类与鉴定[M].北京:中国大地出版社,2009:1-389.
[17]BISSETT J.SZAKACS G,NOLAN C A,et al.Seven new taxa of Trichoderma from Asia[J].Canadian Journal of Botany,2003,81:570-586.
[18]SONG X X,ZHAO Y,SONG C Y,et al.ITS1/5.8S/ITS2,a good marker for initial classification of shiitake culinarymedicinal Lentinus edodes(Agaricomycetes)strains in China[J].International Journal of Medicinal Mushrooms,2018,20(3):227-242.