一种防治大豆疫霉根腐病生物种衣剂的应用研究
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摘要
利用生防菌M1-4与植物激活剂、植物调节剂等复配开发出专用于大豆疫霉根腐病防治的高效种衣。考察其抗病和促生效果表明,生防菌M1-4在种衣剂中不宜超过56d。盆栽试验表明,该种衣剂能够显著降低病情指数,防控效果达到75.2%,地上和地下鲜重分别增加了87%和125%,具有极显著的促生作用。田间试验表明,生物种衣剂能够降低苗期株高,起到壮苗的作用,大豆叶片、叶柄的鲜重在鼓粒期显著提高,大豆叶面积在初花期、盛花期、荚期和鼓粒期均显著高于对照。大田应用表明,在宝泉岭和林甸应用分别使大豆疫霉根腐病的发病率降低了16.61%和22.48%,死苗数下降了3.59%和6.75%,产量提高了30.01%和32.66%。因此,该复合生物种衣剂对大豆根腐病的防效显著,可有效提高大豆产量。
The biological seed coating agent contains the plant activators,plant regulators,and the biocontrol bacteria M1-4 with the inhibitory activity against Phytophthora sojae,and the disease resistance and growthpromoting effects of the agent are measured by pot experiments and field trials.The results showed that bio-control bacteria M1-4 was stable in seed coating for 56 d.Pot experiment showed that the agent could significantly reduce the disease index,and the efficiency of control P.sojae was 75.2%.Meanwhile,the freshweight of above ground and underground increased by 87% and 125%,respectively,and it had significanteffect of growth promoting.Field experiments showed that the agent can reduce the seedling height,andstronger seedlings. The fresh weight of leaf and petiole significantly increase in the drum stage,and leafarea was significantly higher than that of the control in the early flowering,flowering,pod and drum stage.The application of the agent in Baoquanling and Lindian showed that the incidence of P.sojae reduces by16.61% and 22.48%,and the number of dead seedlings reduces by 3.59% and 6.75%,and the yield increases by 30.01% and 32.66%,respectively.It shows that the biological seed coating agent has a significant control effect on soybean root rot and can effectively improve soybean yield.
The biological seed coating agent contains the plant activators,plant regulators,and the biocontrol bacteria M1-4 with the inhibitory activity against Phytophthora sojae,and the disease resistance and growthpromoting effects of the agent are measured by pot experiments and field trials.The results showed that bio-control bacteria M1-4 was stable in seed coating for 56 d.Pot experiment showed that the agent could significantly reduce the disease index,and the efficiency of control P.sojae was 75.2%.Meanwhile,the freshweight of above ground and underground increased by 87% and 125%,respectively,and it had significanteffect of growth promoting.Field experiments showed that the agent can reduce the seedling height,andstronger seedlings. The fresh weight of leaf and petiole significantly increase in the drum stage,and leafarea was significantly higher than that of the control in the early flowering,flowering,pod and drum stage.The application of the agent in Baoquanling and Lindian showed that the incidence of P.sojae reduces by16.61% and 22.48%,and the number of dead seedlings reduces by 3.59% and 6.75%,and the yield increases by 30.01% and 32.66%,respectively.It shows that the biological seed coating agent has a significant control effect on soybean root rot and can effectively improve soybean yield.
引文
[1]周园园,王媛媛,朱晓峰,等.生物种衣剂SN101的研制及其对大豆胞囊线虫病的防效[J].中国油料作物学报,2014,36(4):513-518.
[2]王芳.大豆疫霉根腐病的研究进展及展望[J].养殖技术顾问,2012,6:224-225.
[3]刘世名,李魏,戴良英.大豆疫霉根腐病抗性研究进展[J].大豆科学,2016,35(2):320-329.
[4]代瑞平,刘海.大豆疫霉根腐病研究进展[J].大豆科技,2011,1:20-22.
[5]楼兵干,张炳欣,胡利强.腐霉对甲霜灵抗性测定及其生物防治[J].植物保护学报,2001,28(1):55-60.
[6]崔林开,李井干,陆静,等.大豆疫霉菌对甲霜灵抗性风险的研究[J].南京农业大学学报,2013,36(1):47-51.
[7]闫继辰,王媛媛,朱晓峰,等.诱导大豆抗胞囊线虫病和根腐病的生防细菌研究[J].安徽农业科学,2016,9:134-139.
[8]台莲梅,刘欣.木霉菌对大豆根腐病的生物防治研究[C]//中国植物病理学会2009年学术年会论文集.2009.
[9]黄敏敏.大豆疫病生防放线菌的筛选、鉴定及生防机制初探[D].福州:福建农林大学,2014.
[10]申宏波,姚文秋,于永梅,等.不同类型生物农药对大豆疫霉根腐病的防治效果[J].大豆科学,2011,30(6):1054-1056.
[11]慕康国,刘西莉,白建军,等.种衣剂及其生物学效应[J].种子,1998,6:50-52.
[12]陈立杰,万传浩,朱晓峰,等.Snea253生物种衣剂防治大豆胞囊线虫的研究[J].大豆科学,2011,30(3):459-462.
[13]黄志权,于竞.水稻种子包衣技术[J].种子世界,2016,9:3.
[14]杨峰,娄莹,廖敦平,等.玉米–大豆带状套作行距配置对作物生物量、根系形态及产量的影响[J].作物学报,2015,04:642-650.
[15]宫香伟,刘春娟,冯乃杰,等.S_(3307)和DTA-6对大豆不同冠层叶片光合特性及产量的影响[J].植物生理学报,2017,10:1867-1876.
[16]孙晓慧,徐鹏飞,张淑珍.大豆疫霉根腐病菌游动孢子侵染野生大豆下胚轴的细胞学观察[J].大豆科学,2017,36(3):430-434.
[17]朱文静,伍辉军,高学文.芽孢杆菌对大豆根腐病防治效果研究[J].大豆科学,2011,04:621-625.
[18]刘睿,周园园,闫继辰,等.新型复合型生物种衣剂SN102田间防效研究[J].大豆科学,2017,36(3):435-440.
[19]刘丽,马鸣超,姜昕,等.根瘤菌与促生菌双接种对大豆生长和土壤酶活的影响[J].植物营养与肥料学报,2015,21(3):644-654.
[2]王芳.大豆疫霉根腐病的研究进展及展望[J].养殖技术顾问,2012,6:224-225.
[3]刘世名,李魏,戴良英.大豆疫霉根腐病抗性研究进展[J].大豆科学,2016,35(2):320-329.
[4]代瑞平,刘海.大豆疫霉根腐病研究进展[J].大豆科技,2011,1:20-22.
[5]楼兵干,张炳欣,胡利强.腐霉对甲霜灵抗性测定及其生物防治[J].植物保护学报,2001,28(1):55-60.
[6]崔林开,李井干,陆静,等.大豆疫霉菌对甲霜灵抗性风险的研究[J].南京农业大学学报,2013,36(1):47-51.
[7]闫继辰,王媛媛,朱晓峰,等.诱导大豆抗胞囊线虫病和根腐病的生防细菌研究[J].安徽农业科学,2016,9:134-139.
[8]台莲梅,刘欣.木霉菌对大豆根腐病的生物防治研究[C]//中国植物病理学会2009年学术年会论文集.2009.
[9]黄敏敏.大豆疫病生防放线菌的筛选、鉴定及生防机制初探[D].福州:福建农林大学,2014.
[10]申宏波,姚文秋,于永梅,等.不同类型生物农药对大豆疫霉根腐病的防治效果[J].大豆科学,2011,30(6):1054-1056.
[11]慕康国,刘西莉,白建军,等.种衣剂及其生物学效应[J].种子,1998,6:50-52.
[12]陈立杰,万传浩,朱晓峰,等.Snea253生物种衣剂防治大豆胞囊线虫的研究[J].大豆科学,2011,30(3):459-462.
[13]黄志权,于竞.水稻种子包衣技术[J].种子世界,2016,9:3.
[14]杨峰,娄莹,廖敦平,等.玉米–大豆带状套作行距配置对作物生物量、根系形态及产量的影响[J].作物学报,2015,04:642-650.
[15]宫香伟,刘春娟,冯乃杰,等.S_(3307)和DTA-6对大豆不同冠层叶片光合特性及产量的影响[J].植物生理学报,2017,10:1867-1876.
[16]孙晓慧,徐鹏飞,张淑珍.大豆疫霉根腐病菌游动孢子侵染野生大豆下胚轴的细胞学观察[J].大豆科学,2017,36(3):430-434.
[17]朱文静,伍辉军,高学文.芽孢杆菌对大豆根腐病防治效果研究[J].大豆科学,2011,04:621-625.
[18]刘睿,周园园,闫继辰,等.新型复合型生物种衣剂SN102田间防效研究[J].大豆科学,2017,36(3):435-440.
[19]刘丽,马鸣超,姜昕,等.根瘤菌与促生菌双接种对大豆生长和土壤酶活的影响[J].植物营养与肥料学报,2015,21(3):644-654.