摘要
目的:观察两种补肾法在牵张成骨(DO)中对血管生成及新骨生成的影响,评估成骨效能,并对作用机制进行探讨。方法:50只SD大鼠随机分为5组:补肾阳组、补肾阴组、西药组、模型组和空白组,每组10只。构建胫骨DO模型,给予补肾阳法和补肾阴法干预,4周和8周后分别行X线、HE和Masson染色评估成骨情况,血管造影观察牵张区血管形成,ELISA检测骨髓中VEGF、Ang-2、Runx-2和BMP-2含量水平,qPCR检测骨中对应因子的mRNA表达。结果:X线、HE和Masson染色示补肾阳组和补肾阴组的成骨效果明显优于对照组与模型组,两种补肾法均可提高Lane-Sandhu、Huddleston分值(P<0.05)。与对照组与模型组比较,补肾阳、补肾阴两组可显著增加血管体积分数(P<0.05),上调VEGF、Ang-2和Runx-2、BMP-2及相应mRNA的表达(P<0.05),其中补肾阳组VEGF、Ang-2及其mRNA表达最高,而补肾阴组Runx-2、BMP-2及其mRNA表达最高。结论:补肾阳法和补肾阴法均可上调DO中成血管-成骨因子表达,促进血管生成和新骨形成,从而改善成骨效能、促进骨修复,但二者的作用机制存在差异。
Objective: To observe the effects of two types of tonifying kidney on angiogenesis and new bone formation in distraction osteogenesis(DO), evaluate the osteogenesis efficacy and explore the mechanism. Methods: Fifty 10-week SD rats were randomly divided into five groups: tonifying kidney-yang group, tonifying kidney-yin group, western medicine group, model group and control group. DO models of SD rats were established, and after 4 and 8 weeks, osteogenesis was evaluated by X-ray, HE and Masson staining, respectively. Angiography was used to observe the angiogenesis in the distraction area. The levels of VEGF, Ang-2, Runx-2 and BMP-2 in bone marrow were detected by ELISA. The mRNA expressions of corresponding factors were detected by q PCR. Results: X-ray, HE and Masson staining showed that the osteogenesis effects of tonifying kidney-yang and kidney-yin were significantly better than that of model group and control group(P<0.05). Both kidney-reinforcing methods could improve the LaneSandhu and Huddleston scores(P<0.05) and up-regulate the expressions of VEGF, Ang-2 and Runx-2, BMP-2 and corresponding m RNA(P<0.05). Among them, the expressions of VEGF, Ang-2 and their mRNA were highest in the tonifying kidney-yang group,whilie the expression of Runx-2, BMP-2 and their mRNA were highest in the tonifying kidney-yin group. Conclusion: Both tonifying kidney-yang and kidney-yin could promote the expressions of angiogenic-osteogenic factors, promote angiogenesis and new bone formation, and improve the osteogenic efficacy in DO, but the mechanisms of the two methods are different.
引文
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