不同清洗剂对消化内镜生物膜的清除效果研究
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摘要
目的观察不同清洗剂对消化内镜生物膜的清除效果。方法采用细菌定量检测法和残留蛋白检测法,对不同清洗剂清除模拟内镜管腔人工生物膜的效果进行观察与评价。结果用全效多酶清洗剂、无酶清洗剂和生物膜清洗剂等3组清洗剂清洗后,用细菌培养法检测生物膜清除率依次分别为93. 54%、96. 01%和99. 29%;用残留蛋白检测法检测清除率分别为83. 72%、90. 35%和95. 74%; 3组清除率差异有统计学意义(P <0. 05)。结论本研究3组清洗剂对模拟内镜管腔人工生物膜清除效果,以生物膜专用清洗剂清除效果最好。
Objective To study the cleaning effect for digestive endoscope biofilm by different cleaning agents. Methods The cleaning effect of the biofilm model of endoscope with different cleaning agents were observed and evaluated by means of the bacterial culture and protein quantitative method. Results Using multiple enzymes cleaning agent,no-enzyme cleaning agent and biofilm cleaning agent,the eliminating rates of the bacterial biofilms by means of the bacterial culture were 93. 54%,96. 01% and 99. 29% respectively. The eliminating rates of the bacterial biofilms by means of the protein quantitative were 83. 72% 、90. 35% and 95. 74% respectively,with statistical significant differrence( P < 0. 05). Conclusion The cleaning effect of biofilm cleaning agent was the best among the three cleaning agents on the biofilm model of endoscope.
Objective To study the cleaning effect for digestive endoscope biofilm by different cleaning agents. Methods The cleaning effect of the biofilm model of endoscope with different cleaning agents were observed and evaluated by means of the bacterial culture and protein quantitative method. Results Using multiple enzymes cleaning agent,no-enzyme cleaning agent and biofilm cleaning agent,the eliminating rates of the bacterial biofilms by means of the bacterial culture were 93. 54%,96. 01% and 99. 29% respectively. The eliminating rates of the bacterial biofilms by means of the protein quantitative were 83. 72% 、90. 35% and 95. 74% respectively,with statistical significant differrence( P < 0. 05). Conclusion The cleaning effect of biofilm cleaning agent was the best among the three cleaning agents on the biofilm model of endoscope.
引文
[1]孔金艳,唐平,张修礼,等. 82所医疗机构消化内镜清洗消毒现状调查分析[J].中华医院感染学杂志,2010,20(18):2795-2797.
[2] Nelson DB. Recent advances in epidemiology and prevention of gastrointestinal endoscopy related infections[J]. Curr Opin lnfect Dis,2005,18(4):326-330.
[3] Miner N,Harris V,Ebron T,et al. Sporicidal activity of disinfectants as one possible cause for bacteria in patient-ready endoscopes[J]. Gastroenterol Nurs,2007,30(4):285-290.
[4]赵晓慧.内镜中心医院感染的危险因素分析及对策[J].中华实用诊断与治疗杂志,2013,27(3):226-227.
[5]李仙丽,吕新芝,汤得真.消化内镜多酶清洗方法的改进[J].中国消毒学杂志,2012,29(5):459-460.
[6]李良芳,刘婉薇,郑悦,等. MatrixTM生物膜专用清洗剂用于内镜清洗的效果观察[J].护理研究,2016,30(1):82-84.
[7]夏菁,王悦,常静.一种新型生物膜清洗液清洗效果的临床研究[J].中华医院感染学杂志,2017,27(18):4258-4260.
[8]陈彦丽,史克利,陈烨,等.生物膜特效清洗剂与全效酶对腰椎穿刺针清洗效果对比[J].中国消毒学杂志,2014,31(3):312-313.
[9]马苏,傅增军,丁文霞,等.消化内镜腔细菌生物膜有效清除方法[J].中国消毒学杂志,2017,34(9):807-810.
[10]李铁军,张海燕,赵勇,等.内镜器械应用生物膜清洗剂的清洗效果[J].中国医学装备,2016,13(3):22-24.
[11]苏爱莲,许少英,袁小玲,等.不同温度鲁沃夫多酶溶液对手术器械清洗效果的影响[J].现代医院,2013,13(10):70-71.
[12]张流波,杨华明.医学消毒学最新进展[M].北京:人民军医出版社,2015:183-186.
[2] Nelson DB. Recent advances in epidemiology and prevention of gastrointestinal endoscopy related infections[J]. Curr Opin lnfect Dis,2005,18(4):326-330.
[3] Miner N,Harris V,Ebron T,et al. Sporicidal activity of disinfectants as one possible cause for bacteria in patient-ready endoscopes[J]. Gastroenterol Nurs,2007,30(4):285-290.
[4]赵晓慧.内镜中心医院感染的危险因素分析及对策[J].中华实用诊断与治疗杂志,2013,27(3):226-227.
[5]李仙丽,吕新芝,汤得真.消化内镜多酶清洗方法的改进[J].中国消毒学杂志,2012,29(5):459-460.
[6]李良芳,刘婉薇,郑悦,等. MatrixTM生物膜专用清洗剂用于内镜清洗的效果观察[J].护理研究,2016,30(1):82-84.
[7]夏菁,王悦,常静.一种新型生物膜清洗液清洗效果的临床研究[J].中华医院感染学杂志,2017,27(18):4258-4260.
[8]陈彦丽,史克利,陈烨,等.生物膜特效清洗剂与全效酶对腰椎穿刺针清洗效果对比[J].中国消毒学杂志,2014,31(3):312-313.
[9]马苏,傅增军,丁文霞,等.消化内镜腔细菌生物膜有效清除方法[J].中国消毒学杂志,2017,34(9):807-810.
[10]李铁军,张海燕,赵勇,等.内镜器械应用生物膜清洗剂的清洗效果[J].中国医学装备,2016,13(3):22-24.
[11]苏爱莲,许少英,袁小玲,等.不同温度鲁沃夫多酶溶液对手术器械清洗效果的影响[J].现代医院,2013,13(10):70-71.
[12]张流波,杨华明.医学消毒学最新进展[M].北京:人民军医出版社,2015:183-186.