苦瓜提取物对四种兽医临床常见菌的抑菌活性研究
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摘要
采用打孔法和二倍稀释法研究不同质量浓度的苦瓜提取物对4种临床常见菌的抑制作用,并测定苦瓜提取物对4种菌株生长曲线的影响。结果显示,10 mg/mL的苦瓜提取物对大肠杆菌和沙门氏菌的抑制作用最强,而对链球菌和金黄色葡萄球菌的抑制作用不显著;苦瓜提取物对4种菌株的最小抑菌浓度(MIC)分别为2.5 mg/mL、2.5 mg/mL、5 mg/mL和10 mg/mL。结果提示,苦瓜提取物具有较强的抑菌活性。
This paper studied inhibition effects of different concentrations of Momordica charantia extract on four common bacteria in veterinary clinical by using drilling method and double dilution method, and determined their effects on the growth curve of four bacterial strains. The results showed that 10 mg/mL of Momordica charantia extract exhibit the strongest inhibition on Escherichia coli and Salmonella, but its inhibition on Streptococcus pneumoniae and Staphylococcus aureus was not significant. The minimum inhibitory concentrations(MIC) of Momordica charantia extract against four strains was 2.5 mg/mL, 2.5 mg/mL, 5 mg/mL and 10 mg/mL, respectively. These results suggested that Momordica charantia extract has strong inhibition effect on common bacteria in veterinary clinical.
This paper studied inhibition effects of different concentrations of Momordica charantia extract on four common bacteria in veterinary clinical by using drilling method and double dilution method, and determined their effects on the growth curve of four bacterial strains. The results showed that 10 mg/mL of Momordica charantia extract exhibit the strongest inhibition on Escherichia coli and Salmonella, but its inhibition on Streptococcus pneumoniae and Staphylococcus aureus was not significant. The minimum inhibitory concentrations(MIC) of Momordica charantia extract against four strains was 2.5 mg/mL, 2.5 mg/mL, 5 mg/mL and 10 mg/mL, respectively. These results suggested that Momordica charantia extract has strong inhibition effect on common bacteria in veterinary clinical.
引文
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[5]张雁,李建雄,丘银清,等.苦瓜水提取抑菌作用的初步探讨[J].食品工业科技,2008(12):104-105.
[2]刘锐,赵翾,胡诗娜.苦瓜汁的抑菌活性及其热稳定性的研究[J].食品工业科技,2011,32(5):142-144.
[3]张绪忠,陈青山,程光文.苦瓜抗菌作用的研究[J].中草药,1995,26(10):556.
[4]耿丽晶,周围,张丽艳,等.苦瓜总皂苷最小抑菌浓度和最佳抑菌条件的研究[J].食品工业科技,2012(11):79-82.
[5]张雁,李建雄,丘银清,等.苦瓜水提取抑菌作用的初步探讨[J].食品工业科技,2008(12):104-105.