流动注射-化学发光法测定猪饲料中的吡喹酮
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摘要
基于吡喹酮对Luminol-NaIO_4化学发光体系有较强的抑制作用,并结合流动注射分析技术,建立了测定吡喹酮的流动注射-化学发光(FI-CL)新方法。在最佳实验条件下,吡喹酮在4.0×10~(-8)～1.0×10~(-6) mol/L浓度范围内与相对化学发光强度呈良好的线性关系,该方法对测定吡喹酮显示出较高的灵敏度,检出限(LOD,3σ)为9.9×10~(-9) mol/L。对2.0×10~(-7) mol/L的吡喹酮平行测定11次,其相对标准偏差(RSD)为1.4%。采用该方法对猪饲料样品进行加标实验,回收率为98.5%～100%。该方法已经成功用于猪饲料样品中吡喹酮的测定。
A novel flow injection-chemiluminescence(FI-CL) method was established for the determination of praziquantel based on the strong inhibition of praziquantel on Luminol-NaIO_4 chemiluminescence system.Under the optimal experimental conditions,there was a good linear relationship between relative intensity of chemiluminescence and praziquantel in the concentration range of 4.0×10~(-8)-1.0×10~(-6) mol/L,with a limit of detection(LOD) of 9.9×10~(-9) mol/L.The relative standard deviation(RSD,n=11) was 1.4% for the determination of 2.0×10~(-7) mol/L praziquantel.The proposed method was used for the detection of praziquantel in the spiked pig feed sample with recoveries of 98.5%-100%.The method was successfully applied in the determination of praziquantel in pig feed with satisfactory results.
A novel flow injection-chemiluminescence(FI-CL) method was established for the determination of praziquantel based on the strong inhibition of praziquantel on Luminol-NaIO_4 chemiluminescence system.Under the optimal experimental conditions,there was a good linear relationship between relative intensity of chemiluminescence and praziquantel in the concentration range of 4.0×10~(-8)-1.0×10~(-6) mol/L,with a limit of detection(LOD) of 9.9×10~(-9) mol/L.The relative standard deviation(RSD,n=11) was 1.4% for the determination of 2.0×10~(-7) mol/L praziquantel.The proposed method was used for the detection of praziquantel in the spiked pig feed sample with recoveries of 98.5%-100%.The method was successfully applied in the determination of praziquantel in pig feed with satisfactory results.
引文
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[4] The Japan Food Chemical Research Foundation Maximum Residue Limits(MRLs) List of Agricultura Chemicals in Foods,[2014-01-30].http://www.m5.ws001.squarestart.ne.jp/foundation/search.html.
[5] Babes R M,Selescu T,Domocos D,Babes A.Toxicol.Appl.Pharm.,2017,336:55-65.
[6] He L,Gao F,Li E,Tzuoh L J,Bian L,Armstrong D W.Talanta,2017,174:380-386.
[7] Malhado M,Pinto D P,Silva A C,Silveira G P,Pereira H M,Santos J G,Jr Guilarducci F C V,Vicosa A L,Nele M,Fonseca L B,Pinto J C,Calil E S.J.Pharm.Biomed.,2016,117:405-412.
[8] Su M M,Yang L,Zhang Y G,Jiang Y.J.Food Saf.Qual.(苏明明,杨璐,张颖刚,蒋莹.食品安全质量检测学报),2017,8(6):2263-2266.
[9] Hou G C,Tan A J,Lv S M,Jin X F,Zhang J Y.Food Sci.(侯改成,谭艾娟,吕世明,金晓峰,张剑勇.食品科学),2015,36(6):216-219.
[10] Zheng J,Wang X B,Xu Z Z.J.Pharm.Res.(郑静,王小兵,徐志洲.药学研究),2014,33(3):145-147.
[11] Piantavini M S,Pontes F L D,Uber C P,Stremel D P,Sena M M,Pontarolo R.Spectrochim.Acta A,2014,125:396-403.
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[13] Xu T,Zhang Y T,Zhang X.J.Instrum.Anal.(徐通,张琰图,张绪.分析测试学报),2017,36(6):805-808.
[14] Lin Y N,Li J B,Wang Y H,Sun Y L,Ding C F,Sun W Y,Luo C N.Spectrochim.Acta A,2018,192:153-158.
[15] Sheng Y Y,Yang H L,Wang Y,Han L,Zhao Y J,Fan A P.Talanta,2017,166:268-274.
[16] Hananya N,Boock A E,Bauer C R,Fainaro S R,Shabat D.J.Am.Chem.Soc.,2016,138(40):13438-13446.
[17] Zhang Y,Yang C Y,Shu Y Q,Chen F B,Chang Z Q ,Zhang Z J.Chin.J.Anal.Lab.(张玉,杨春艳,舒砚勤,陈复彬,常自强,章竹君.分析试验室),2016,35(3):283-286.
[18] Asghar M,Yaqoob M,Nabi A.Chem.Res.Chin.Univ.,2017,33(3):354-359.
[19] Vakh C,Evdokimova E,Pochivalov A,Leonid M,Bulatov A.Food Chem.,2017,237:929-935.
[20] Li Q Q,Zhang L J,Li J G,Lu C.TrAC-Trend Anal.Chem.,2011,30(2):401-413.
[21] Li S F,Li X Z,Xu J,Wei X W.Talanta,2008,75(1):32-37.
[22] Shi M,Huang Y,Zhao J J,Li S T,Liu R J,Zhao S L.Talanta,2018,179:466-471.
[23] Li M,Zhang Y Y,Xue Y L,Hong X,Gui L,Liu Z J,Du T L.Food Control,2017,73:1039-1044.