不同地理株柔嫩艾美耳球虫(E.tenela)杂交虫株(F2)的培育
摘要
将山东株(S)与吉林株(J)柔嫩艾美耳球虫孢子化卵囊通过单卵囊技术接种雏鸡,收获卵囊后提取基因组DNA,利用RAPD技术分析DNA,筛选出杂交株(F1),再用该F1代与黑龙江株(H)柔嫩艾美耳球虫孢子化卵囊单卵囊接种雏鸡,相同方法鉴别筛选,确定了1株为山东株、吉林株和黑龙江株三个地理株的杂交(F2)卵囊。该结果为深入研究球虫的免疫机制奠定了基拙。
引文
[1]康桂英.鸡球虫病的免疫预防研究进展[J].中国畜牧兽医文摘,2015(11):155~156.
[2]高均伟,龚睿,张轶,等.鸡球虫病常见防治药物及其抗虫机理[J].上海畜牧兽医通讯,2014(6):76~79.
[3]Rose,M.E,and hesketh,P.Infections with Eimeria species:the role of bile[J].J.Parasitol,1983:69:439~440.
[4]Schiotani,N.,Baba,E.,Fukata,T.,Arakawa,A.and Nakanishi,T.Distribution of oocyst,sporocysts and sporozoites of Eineriatenella and Eimeria maxima in the digestire tract of chicken[J].Veterinary Parasitology.1992;41:17~22.
[5]杨楠,宁长申,齐萌,等.鸡巨型艾美耳球虫单卵囊分离及雏鸡扩增试验[J].中国畜牧兽医,2011(6):172~174.
[6]索勋,李国清.鸡球虫病学[M].北京:中国农业大学出版社,1997.
[7]张西臣,赵权,等.动物寄生虫病学[M].长春:吉林人民出版社,2001.
[8]蒋建林,蒋金书.柔嫩艾美耳球虫各阶段虫体纯化方法的改进[J].中国农业大学学报,1996,1(5):99~102.
[9]陈兆国,史天卫,等.用RAPD技术进行鸡球虫不同种株的多态性研究[A].球虫论文集[M].中国农业科学院上海家畜寄生虫病研究所,2002:80~86.
[10]Wisher M H,Rose M E.The large-scale preparation of purified sporozoites of Eomeria spp by metriamide density-gradient centeifugation[J].Parasitology,1984;88;515~519.
[11]Stotish R L,Wang C C.Preparation of merozoites of Eimeria tenella[J].J Parasitol,1975;61(1):700703.
[12]Stotish R L,et al.Separation of sporozoites and oocysts of Eimeria tenella by centrifugal elutriation[J].J Parasitol,1997;63(6):1124~1126.
[13]Vetterling J M.Continuous-flow differential fendity flotation of coccidial oocysts and a comparetion with other methods[J].J Parasitol,1996;55(2):412~417.
[14]Xie M Q et al.A new method for purification of Eimeria tenella Merozoites[J].Parasitol.Res,1990;76:566~569.
[15]Shirley,M.W.A novel method for cloning E.praecox in the chicken[J].Parasitol,1989,75:324.
[16]Chapman.H.D.Cloning of Eimeria tenella in the chicken[J].Parasitol,1986,72:605.
[17]赵权.柔嫩艾美耳球虫杂交株F2培育、特异基因的克隆、鉴定及分析[D].[博士学位论文].长春:解放军军需大学,2004.
[2]高均伟,龚睿,张轶,等.鸡球虫病常见防治药物及其抗虫机理[J].上海畜牧兽医通讯,2014(6):76~79.
[3]Rose,M.E,and hesketh,P.Infections with Eimeria species:the role of bile[J].J.Parasitol,1983:69:439~440.
[4]Schiotani,N.,Baba,E.,Fukata,T.,Arakawa,A.and Nakanishi,T.Distribution of oocyst,sporocysts and sporozoites of Eineriatenella and Eimeria maxima in the digestire tract of chicken[J].Veterinary Parasitology.1992;41:17~22.
[5]杨楠,宁长申,齐萌,等.鸡巨型艾美耳球虫单卵囊分离及雏鸡扩增试验[J].中国畜牧兽医,2011(6):172~174.
[6]索勋,李国清.鸡球虫病学[M].北京:中国农业大学出版社,1997.
[7]张西臣,赵权,等.动物寄生虫病学[M].长春:吉林人民出版社,2001.
[8]蒋建林,蒋金书.柔嫩艾美耳球虫各阶段虫体纯化方法的改进[J].中国农业大学学报,1996,1(5):99~102.
[9]陈兆国,史天卫,等.用RAPD技术进行鸡球虫不同种株的多态性研究[A].球虫论文集[M].中国农业科学院上海家畜寄生虫病研究所,2002:80~86.
[10]Wisher M H,Rose M E.The large-scale preparation of purified sporozoites of Eomeria spp by metriamide density-gradient centeifugation[J].Parasitology,1984;88;515~519.
[11]Stotish R L,Wang C C.Preparation of merozoites of Eimeria tenella[J].J Parasitol,1975;61(1):700703.
[12]Stotish R L,et al.Separation of sporozoites and oocysts of Eimeria tenella by centrifugal elutriation[J].J Parasitol,1997;63(6):1124~1126.
[13]Vetterling J M.Continuous-flow differential fendity flotation of coccidial oocysts and a comparetion with other methods[J].J Parasitol,1996;55(2):412~417.
[14]Xie M Q et al.A new method for purification of Eimeria tenella Merozoites[J].Parasitol.Res,1990;76:566~569.
[15]Shirley,M.W.A novel method for cloning E.praecox in the chicken[J].Parasitol,1989,75:324.
[16]Chapman.H.D.Cloning of Eimeria tenella in the chicken[J].Parasitol,1986,72:605.
[17]赵权.柔嫩艾美耳球虫杂交株F2培育、特异基因的克隆、鉴定及分析[D].[博士学位论文].长春:解放军军需大学,2004.