褐根病菌分子检测与生物防治研究进展
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摘要
褐根病是由有害木层孔菌Phellinus noxius引起的林木和多年生果树的重要根部病害。通过综述了近年来有关褐根病菌的单克隆抗体检测法等4种分子检测技术和生防放线菌等4种生物防治的研究,指出由于分子检测方法的敏感度和特异度高,可用于早期诊断褐根病菌;同时也表明用生物防治替代传统的化学防治进行褐根病菌防治,并使生物制剂有效推广运用,将是今后研究的热点和控制该病害的主要手段。
Brown root rot disease, caused by Phellinus noxius, has become an important disease of forest and perennial fruit trees. This paper summarizes 4 kinds of molecular detections such as monoclonal antibody detection and 4 kinds of biological control such as actinomyces of brown root rot disease. It points out that molecular detection will be feasibly early diagnostic tool for brown root rot disease due to its high sensitivity and specificity. And it shows that it will be focus of biological control instead of the traditional chemical methods to control brown root rot disease and major measures of application of biological control preparation effectively in the future.
Brown root rot disease, caused by Phellinus noxius, has become an important disease of forest and perennial fruit trees. This paper summarizes 4 kinds of molecular detections such as monoclonal antibody detection and 4 kinds of biological control such as actinomyces of brown root rot disease. It points out that molecular detection will be feasibly early diagnostic tool for brown root rot disease due to its high sensitivity and specificity. And it shows that it will be focus of biological control instead of the traditional chemical methods to control brown root rot disease and major measures of application of biological control preparation effectively in the future.
引文
[1]潘浣钰,彭少麟,张素梅,等.土壤理化性质与褐根病感染的相互关系[J].生态环境,2008(17):1650-1653.
[2]林石明,廖富荣,陈红运,等.台湾褐根病发生情况及研究进展[J].植物检疫,2012(26):54-60.
[3]Ann P,Lee H,Huang T.Brown root rot of 10 species of fruit trees caused by Phellinus noxius in Taiwan[J].Plant Disease,1999,83:746-750.
[4]Huang H,Sun L,Bi K,et al.The Effect of Phenazine-1-Carboxylic Acid on the Morphological,Physiological,and Molecular Characteristics of Phellinus noxius[J].Molecules,2016;21:613.
[5]毕可可,阮琳,代色平,等.10种杀菌剂对树木褐根病菌的室内抑菌试验.中国观赏园艺研究进展[C],北京:中国林业出版社,2012,705-707.
[6]Ann P-J,Chang T-T,Ko W-H.Phellinus noxius brown root rot of fruit and ornamental trees in Taiwan[J].Plant Disease,2002,86:820-826.
[7]梁艳琼,吴伟怀,李锐,等.橡胶树褐根病菌的分子检测[J].热带作物学报,2016,37:576-581.
[8]蔡志浓,谢文瑞,安宝贞,等.褐根病菌Phellinus noxius检测用专一性引子对的开发[J].植病会刊,2007(16):193-202.
[9]孙艳秋,赵奎华,刘长远,等.快速检测黄瓜细菌性角斑病菌的间接ELISA法优化[J].沈阳农业大学学报,2014(2):213-216.
[10]吴孟玲,洪挺轩,蔡佳欣,等.树木褐根病单元抗体检测技术的建立[J].中华林业季刊,2010(43):191-199.
[11]刘旭光,宋福平,张杰.寡核苷酸芯片在微生物检测中的应用[J].生物技术通报,2005(1):29-33.
[12]Tzean Y,Shu PY,Liou RF,et al.Development of oligonucleotide microarrays for simultaneous multi-species identification of Phellinus tree-pathogenic fungi[J].Microbial biotechnology,2016,9:235-244.[1 3]H e n s o n J M,F r e n c h R.T h e polymerase chain reaction and plant disease diagnosis[J].Annual review of phytopathology,1993,31:81-109.
[14]Cook KL,Layton AC,Dionisi HM,et al.Evaluation of a plasmid-based 16S-23S r DNA intergenic spacer region array for analysis of microbial diversity in industrial wastewater[J].Journal of microbiological methods,2004,57:79-93.
[15]吴孟玲,张东柱,庄铃木.树木褐根病PCR检测技术的建立[J].中华林业季刊,2009(42):239-247.
[16]Wang Y-F,Meng H,Gu VW,et al.Molecular diagnosis of the brown root rot disease agent Phellinus noxius on trees and in soil by r DNA ITS analysis[J].Applied Environmental Biotechnology,2016,1(1):81–91.
[17]Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic acids research,2000,28:E63.
[18]黄裕星,陈昭翰,洪挺轩,等.树木褐根病的恒温环状扩增法(LAMP)检测方法的研发[J].植病会刊,2014(23):1-13.
[19]姜钰,董怀玉,徐秀德,等.放线菌在植病生防中的研究进展[J].杂粮作物,2005(25):329-331.
[20]Kothandaraman R,Joseph K,Mathew J,et al.Actinomycete population in the rhizosphere of Hevea and its inhibitory effect on Phellinus noxius[J].Indian Journal of Natural Rubber Research,1991,4:150-152.
[21]Yanti Y,Zainon MN,Marshida AHU.Antagonistic activity of three Actinomycetes,MG01,MG02 And KT2F towards Phellinus noxius.Business,Engineering and Industrial Applications(ISBEIA)(C).2012,729-732.
[22]Harman GE.Overview of Mechanisms a n d U s e s o f T r i c h o d e r m a s p p[J].Phytopathology,2006,96:190-194.
[23]倪蕙芳,许淑丽,陈瑞祥,等.台湾地区土壤中木霉菌株对植物病原真菌拮抗能力的筛选[J].台湾农业研究,2010(59):29-41.
[24]Schwarze FW,Jauss F,Spencer C,et al.Evaluation of an antagonistic Trichoderma strain for reducing the rate of wood decomposition by the white rot fungus Phellinus noxius[J].Biological Control,2012,61:160-168.
[25]李和平,唐朝荣,阳美芳,等.木霉菌对橡胶树褐根病菌的拮抗作用[J].福建农业学报,2015(30):362-366.
[26]李明,双宝,李海涛,等.枯草芽孢杆菌的研究与应用[J].东北农业大学学报,2009(40):111-114.
[27]赵璐璐,贺春萍,郑肖兰,等.枯草芽孢杆菌Czk1菌株对橡胶树根病菌的抑制作用及对炭疽病生防效果研究初报[J].南方农业学报,2011(42):740-743.
[28]Chakraborty U,Chakraborty B,Basnet M,et al.Evaluation of Ochrobactrum anthropi TRS-2 and its talc based formulation for enhancement of growth of tea plants and management of brown root rot disease[J].Journal of applied microbiology,2009,107:625-634.
[29]佘俊弘.褐根菌抑制机制之研究[D].台湾:国立成功大学,2012.
[30]吴孟玲,许育晏,李芷芸,等.微生物制剂在褐根病管理的应用研究[J].台湾林业科学,2014(29):41-53.
[31]毕可可,谭凤圆,吴超,等.一株拮抗树木褐根病菌的伯克霍尔德菌的分离及鉴定[J].林业与环境科学,2016,32(4):7-11.
[32]张东柱.认识树木褐根病及其防治.植物重要防疫检疫病害诊断鉴定技术研习会专刊[C].台北:中华林学会,2007:39-51.
[33]蔡志浓,安宝贞,谢文瑞.抑制褐根病菌、白纹羽菌及南方灵芝菌的化学药剂筛选[J].植病会刊,2005(14):115-124.
[34]Fu CA,Hu BA,Chang TA,et al.Evaluation of dazomet as fumigant for the control of brown root rot disease[J].Pest management science,2012,68:959-962.
[2]林石明,廖富荣,陈红运,等.台湾褐根病发生情况及研究进展[J].植物检疫,2012(26):54-60.
[3]Ann P,Lee H,Huang T.Brown root rot of 10 species of fruit trees caused by Phellinus noxius in Taiwan[J].Plant Disease,1999,83:746-750.
[4]Huang H,Sun L,Bi K,et al.The Effect of Phenazine-1-Carboxylic Acid on the Morphological,Physiological,and Molecular Characteristics of Phellinus noxius[J].Molecules,2016;21:613.
[5]毕可可,阮琳,代色平,等.10种杀菌剂对树木褐根病菌的室内抑菌试验.中国观赏园艺研究进展[C],北京:中国林业出版社,2012,705-707.
[6]Ann P-J,Chang T-T,Ko W-H.Phellinus noxius brown root rot of fruit and ornamental trees in Taiwan[J].Plant Disease,2002,86:820-826.
[7]梁艳琼,吴伟怀,李锐,等.橡胶树褐根病菌的分子检测[J].热带作物学报,2016,37:576-581.
[8]蔡志浓,谢文瑞,安宝贞,等.褐根病菌Phellinus noxius检测用专一性引子对的开发[J].植病会刊,2007(16):193-202.
[9]孙艳秋,赵奎华,刘长远,等.快速检测黄瓜细菌性角斑病菌的间接ELISA法优化[J].沈阳农业大学学报,2014(2):213-216.
[10]吴孟玲,洪挺轩,蔡佳欣,等.树木褐根病单元抗体检测技术的建立[J].中华林业季刊,2010(43):191-199.
[11]刘旭光,宋福平,张杰.寡核苷酸芯片在微生物检测中的应用[J].生物技术通报,2005(1):29-33.
[12]Tzean Y,Shu PY,Liou RF,et al.Development of oligonucleotide microarrays for simultaneous multi-species identification of Phellinus tree-pathogenic fungi[J].Microbial biotechnology,2016,9:235-244.[1 3]H e n s o n J M,F r e n c h R.T h e polymerase chain reaction and plant disease diagnosis[J].Annual review of phytopathology,1993,31:81-109.
[14]Cook KL,Layton AC,Dionisi HM,et al.Evaluation of a plasmid-based 16S-23S r DNA intergenic spacer region array for analysis of microbial diversity in industrial wastewater[J].Journal of microbiological methods,2004,57:79-93.
[15]吴孟玲,张东柱,庄铃木.树木褐根病PCR检测技术的建立[J].中华林业季刊,2009(42):239-247.
[16]Wang Y-F,Meng H,Gu VW,et al.Molecular diagnosis of the brown root rot disease agent Phellinus noxius on trees and in soil by r DNA ITS analysis[J].Applied Environmental Biotechnology,2016,1(1):81–91.
[17]Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic acids research,2000,28:E63.
[18]黄裕星,陈昭翰,洪挺轩,等.树木褐根病的恒温环状扩增法(LAMP)检测方法的研发[J].植病会刊,2014(23):1-13.
[19]姜钰,董怀玉,徐秀德,等.放线菌在植病生防中的研究进展[J].杂粮作物,2005(25):329-331.
[20]Kothandaraman R,Joseph K,Mathew J,et al.Actinomycete population in the rhizosphere of Hevea and its inhibitory effect on Phellinus noxius[J].Indian Journal of Natural Rubber Research,1991,4:150-152.
[21]Yanti Y,Zainon MN,Marshida AHU.Antagonistic activity of three Actinomycetes,MG01,MG02 And KT2F towards Phellinus noxius.Business,Engineering and Industrial Applications(ISBEIA)(C).2012,729-732.
[22]Harman GE.Overview of Mechanisms a n d U s e s o f T r i c h o d e r m a s p p[J].Phytopathology,2006,96:190-194.
[23]倪蕙芳,许淑丽,陈瑞祥,等.台湾地区土壤中木霉菌株对植物病原真菌拮抗能力的筛选[J].台湾农业研究,2010(59):29-41.
[24]Schwarze FW,Jauss F,Spencer C,et al.Evaluation of an antagonistic Trichoderma strain for reducing the rate of wood decomposition by the white rot fungus Phellinus noxius[J].Biological Control,2012,61:160-168.
[25]李和平,唐朝荣,阳美芳,等.木霉菌对橡胶树褐根病菌的拮抗作用[J].福建农业学报,2015(30):362-366.
[26]李明,双宝,李海涛,等.枯草芽孢杆菌的研究与应用[J].东北农业大学学报,2009(40):111-114.
[27]赵璐璐,贺春萍,郑肖兰,等.枯草芽孢杆菌Czk1菌株对橡胶树根病菌的抑制作用及对炭疽病生防效果研究初报[J].南方农业学报,2011(42):740-743.
[28]Chakraborty U,Chakraborty B,Basnet M,et al.Evaluation of Ochrobactrum anthropi TRS-2 and its talc based formulation for enhancement of growth of tea plants and management of brown root rot disease[J].Journal of applied microbiology,2009,107:625-634.
[29]佘俊弘.褐根菌抑制机制之研究[D].台湾:国立成功大学,2012.
[30]吴孟玲,许育晏,李芷芸,等.微生物制剂在褐根病管理的应用研究[J].台湾林业科学,2014(29):41-53.
[31]毕可可,谭凤圆,吴超,等.一株拮抗树木褐根病菌的伯克霍尔德菌的分离及鉴定[J].林业与环境科学,2016,32(4):7-11.
[32]张东柱.认识树木褐根病及其防治.植物重要防疫检疫病害诊断鉴定技术研习会专刊[C].台北:中华林学会,2007:39-51.
[33]蔡志浓,安宝贞,谢文瑞.抑制褐根病菌、白纹羽菌及南方灵芝菌的化学药剂筛选[J].植病会刊,2005(14):115-124.
[34]Fu CA,Hu BA,Chang TA,et al.Evaluation of dazomet as fumigant for the control of brown root rot disease[J].Pest management science,2012,68:959-962.