摘要
以刺梨(Rosa roxburghii Trat)为原料,采用正交试验优化刺梨多酚氧化酶(polyphenol oxidase,PPO)的提取条件,并研究不同抑制剂[抗坏血酸、乙二胺四乙酸二钠(ethylenediaminetetraacetic acid disodium salt,EDTA-2Na)、柠檬酸]对刺梨PPO活性的影响。结果表明,PPO的最佳提取工艺条件为:底物邻苯二酚的浓度为0.12 mol/L,提取温度为30℃,缓冲液的p H值为6.0。同时,抗坏血酸、EDTA-2Na、柠檬酸对刺梨PPO活性均有抑制效果,其中抗坏血酸对PPO活性抑制效果最强,其次是柠檬酸、EDTA-2Na。
Using the fruit of Rosa roxburghii Tratt as raw material,orthogonal experiment was used to optimize the extraction conditions of polyphenol oxidase(PPO) in Rosa roxburghii Tratt,and the effects of different inhibitors [ascorbic acid,ethylenediaminetetraacetic acid disodium salt(EDTA-2 Na)and citric acid]on PPO activity in Rosa roxburghii Tratt were studied. The experimental results showed that the optimal extraction conditions of PPO were as follows: the catechol concentration assubstrate 0.12 mol/L, the extraction temperature 30 ℃,and the buffer pH value 6.0. Meanwhile,all the compounds such as ascorbic acid,EDTA-2Na and citric acid,had inhibiting effects on PPO activity in Rosa roxburghii Tratt,among which ascorbic acid had the strongest inhibiting effect on PPO activity,followed by citric acid and EDTA-2Na.
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