微波辅助法对松针水提取液抗氧化性的影响研究
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摘要
以松针水提液为研究对象,采用分光光度法,以羟自由基清除率为指标,通过正交实验优化了微波功率,微波处理时间,氯化钠百分含量等因素对松针水提液抗氧化活性的影响。结果表明,微波功率对松针水提液的抗氧化性影响最大,氯化钠百分含量影响最小;最优实验条件为微波处理功率420 W,微波辐射时间3 min,氯化钠百分含量4%,在此条件下羟自由基清除率达到94. 17%。
The effects of microwave power,microwave treatment time and sodium chloride percentage on the antioxidant activity of pine needle water extract were optimized by orthogonal test by using spectrophotometry with hydroxyl radical scavenging rate as index. The results showed that microwave power had the greatest effect on the antioxidant activity of pine needle water extract and the smallest effect on the content of sodium chloride. The optimum experimental conditions were 420 W microwave power,3 min microwave radiation time and 4% sodium chloride content. Under these conditions,the hydroxyl radical scavenging rate reached 94. 17%.
The effects of microwave power,microwave treatment time and sodium chloride percentage on the antioxidant activity of pine needle water extract were optimized by orthogonal test by using spectrophotometry with hydroxyl radical scavenging rate as index. The results showed that microwave power had the greatest effect on the antioxidant activity of pine needle water extract and the smallest effect on the content of sodium chloride. The optimum experimental conditions were 420 W microwave power,3 min microwave radiation time and 4% sodium chloride content. Under these conditions,the hydroxyl radical scavenging rate reached 94. 17%.
引文
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[2]王婷,徐俞悦,等.松针的保健功效及相关产品研究进展[J].中草药,2018,49(17):4172-4173.
[3]尚军华,吴国良,魏新娜,等.园艺植物多酚物质生理功能及应用研究进展[J].河南农业,2016(24):50-52.
[4]张晓静,龚婷,扶庆权,等.响应面法优化微波辅助提取苹果皮中总黄酮的工艺研究[J].甘肃农业大学学报,2015,50(6):148-155.
[5]吴菲菲,赵良忠,徐永忠,等.以氯化钠为助剂的微切变助剂互作技术辅助提取柑橘皮香精油的研究[J].食品安全质量检测学报,2016,7(8):3246-3252.
[6]林洪斌,曹东,陈燕,等.微波辅助水蒸气法提取柠檬精油工艺研究[J].中国酿造,2015,34(3):76-79.
[7]杨豆,张卫波.微波-加盐水蒸气蒸馏提取玫瑰精油工艺研究[J].中国洗涤用品工业,2017(8):39-41.
[8]张静,姚芳,马维新,等.柚皮精油的微波辅助提取工艺及其抗氧化研究[J].中国食品添加剂,2015(7):58-64.
[9]马承惠,等. 7种松科植物松针提取物的体外抗氧化活性比较[J].东北林业大学学报,2016,44(6):46-47.