β-榄香烯对糖尿病性视网膜病变增殖期大鼠视网膜中VEGF表达的干预作用
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摘要
目的观察β-榄香烯对链脲佐菌素(Streptozocin,STZ)导致增殖性糖尿病性视网膜病变大鼠视网膜中血管内皮生长因子(VEGF)水平的表达,探讨其对增殖性糖尿病性视网膜病变大鼠视网膜的可能作用机制。方法选取80只Wistar大鼠,随机分为正常对照组(NC组)、STZ诱导成功的糖尿病模型组(DC组)、低剂量β-榄香烯给药组(BL组)与高剂量给药组(BH),各20只。给药组分别在手术显微镜下玻璃体腔内注射不同计量的β-榄香烯、苏木精-伊红染色,观察大鼠视网膜组织的病理学改变,取各实验分组中的视网膜切片,对VEGF采用免疫组织化学染色法,检测视网膜组织中VEGF的表达。结果 HE染色结果显示,NC组视网膜各组织结构显现清晰的层次,DC组较NC组视网膜变薄,且结构破坏,神经节细胞数减少;BL组较DC组略有提高;BH组视网膜结构较BL组有好转。免疫组织化学染色后,检测的各实验组视网膜结果显示,DC组、NC组、BL组、BH组大鼠VEGF阳性细胞核数目分别为(125.4671±8.4561)、(10.1324±2.0334)、(75.5123±7.6507)、(21.5364±1.7861)个,各组间差异均有统计学意义(P<0.05)。结论β-榄香烯可能成为治疗增殖期糖尿病性视网膜病变的新作用靶点,同时可以改善晚期糖尿病性视网膜病变的发展,延缓视网膜组织的进一步恶化。
Objective To observe the effect of β-elemene on the expression of vascular endothelial growth factor(VEGF) in the retina of rats with proliferative diabetic retinopathy caused by streptozocin(STZ),to investigate its possible mechanism of action on retinopathy of rats with proliferative diabetic retinopathy.Methods A total of 80 Wistar rats were selected.They were randomly divided into normal control group(NC group),STZ-induced diabetes model group(DC group),low-dose β-elemene group(BL group) and high-dose group(BH group),20 rats in each group.In the administration group,different doses of β-elemene and hematoxylin-eosin staining were injected into the vitreous cavity under the surgical microscope respectively.The pathological changes of rat retina tissues were observed.Retinal slices from each experiment group were collected.Immunohistochemical staining was applied to VEGF,and the expression of VEGF in retinal tissues was detected.Results The results of HE staining showed that the tissue structure of the retina in the NC group showed a clear level.The retina was thinner in DC group than that in the NC group,and the structure was broken.The number of ganglion cells was reduced;the BL group had a slight increase compared with the DC group.The retinal structure in the BH group was better than that in the BL group.After immunohistochemical staining,the retinal results in each experimental group showed that the number of VEGF-positive nuclei in the DC group,NC group,BL group and BH group was(125.4671 ±8.4561),(10.1324 ±2.0334),(75.5123 ±7.6507),(21.5364 ±1.7861) respectively,differences betweenthe groups were statistically significant(P <0.05).Conclusion β-elemene may become a new target for the treatment of proliferative diabetic retinopathy.At the same time,it can improve the development of advanced DR and delay the further deterioration of retinal tissue.
Objective To observe the effect of β-elemene on the expression of vascular endothelial growth factor(VEGF) in the retina of rats with proliferative diabetic retinopathy caused by streptozocin(STZ),to investigate its possible mechanism of action on retinopathy of rats with proliferative diabetic retinopathy.Methods A total of 80 Wistar rats were selected.They were randomly divided into normal control group(NC group),STZ-induced diabetes model group(DC group),low-dose β-elemene group(BL group) and high-dose group(BH group),20 rats in each group.In the administration group,different doses of β-elemene and hematoxylin-eosin staining were injected into the vitreous cavity under the surgical microscope respectively.The pathological changes of rat retina tissues were observed.Retinal slices from each experiment group were collected.Immunohistochemical staining was applied to VEGF,and the expression of VEGF in retinal tissues was detected.Results The results of HE staining showed that the tissue structure of the retina in the NC group showed a clear level.The retina was thinner in DC group than that in the NC group,and the structure was broken.The number of ganglion cells was reduced;the BL group had a slight increase compared with the DC group.The retinal structure in the BH group was better than that in the BL group.After immunohistochemical staining,the retinal results in each experimental group showed that the number of VEGF-positive nuclei in the DC group,NC group,BL group and BH group was(125.4671 ±8.4561),(10.1324 ±2.0334),(75.5123 ±7.6507),(21.5364 ±1.7861) respectively,differences betweenthe groups were statistically significant(P <0.05).Conclusion β-elemene may become a new target for the treatment of proliferative diabetic retinopathy.At the same time,it can improve the development of advanced DR and delay the further deterioration of retinal tissue.
引文
[1]陈子畅,袁进萍,樊芯.雷珠单抗与康柏西普玻璃体腔注射治疗糖尿病黄斑水肿的效果对比[J].宁夏医学杂志,2016,38(12):1147-1149.
[2]周赟,刘宁宁,万超,等.β-榄香烯对糖尿病大鼠视网膜中肿瘤坏死因子-α表达的影响[J].眼科新进展,2015,35(12):1129-1131,1136.
[3]肖津安,白清玉,谢安明.早期糖尿病视网膜病变模型大鼠玻璃体腔注射安维汀后房水细胞因子变化[J].中国组织工程研究,2015,19(18):2933-2939.
[4]姚微微.抗CXCR4单克隆抗体对糖尿病大鼠血-视网膜屏障及视网膜血管内皮生长因子表达的影响[D].桂林:桂林医学院,2015.
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[6]董白霞,叶存喜,韩瑶,等.二十碳五烯酸对增殖性糖尿病性视网膜病变动物模型视网膜组织的超微结构改变的影响[J].河北医科大学学报,2015,36(6):677-681.
[7]李琴,杨安怀.玻璃体腔注射Lucentis联合治疗增殖性糖尿病性视网膜病变合并虹膜新生血管的临床观察[J].武汉大学学报(医学版),2016,37(6):998-1001,1017.
[8]刘青,艾明.雷珠单抗联合全视网膜光凝辅助玻璃体切割手术治疗糖尿病视网膜病变[J].国际眼科杂志,2016,16(9):1637-1640.
[9]李秀云.康柏西普联合低分子肝素辅助玻璃体切割术治疗PDR的长期疗效观察[D].济南:山东大学,2017.
[10]孙荻芳.糖尿病性视网膜病变玻璃体切除术后渗出性视网膜脱离相关因素分析[D].长春:吉林大学,2017.
[11]李康.脑红蛋白在增殖性糖尿病性视网膜病变患者眼部及血清中的表达及临床意义[D].太原:山西医科大学,2016.
[12]张招德.康柏西普辅助玻璃体手术治疗增殖性糖尿病性视网膜病变的临床疗效[J].中外医疗,2016,35(36):73-75.
[13]耿金,陈蕾.β-榄香烯抑制新生血管及在眼科疾病中的应用[J].解剖科学进展,2017,23(3):317-319.
[14]Pedinielli A,Bonnin S,Sanharawi ME,et al.Three different optical coherence tomography angiography measurement methods for assessing capillary density changes in diabetic retinopathy[J].Ophthalmic Surg Lasers Imaging Retina,2017,48(5):378-384.
[15]梁道桐,陈达钻,黄立,等.稳定控制血糖联合康柏西普玻璃体腔注射治疗增殖性糖尿病性视网膜病变临床观察[J].内科,2017,12(4):458-460.
[16]陆相庆.眼底激光治疗重度非增殖性糖尿病性视网膜病变疗效探讨[J].糖尿病新世界,2016,19(1):102-104.
[17]娄静,谢婷玉,陈雪艺.糖尿病大鼠眼内VEGF阶段性表达的Meta分析[J].国际眼科杂志,2015,15(10):1749-1752.
[2]周赟,刘宁宁,万超,等.β-榄香烯对糖尿病大鼠视网膜中肿瘤坏死因子-α表达的影响[J].眼科新进展,2015,35(12):1129-1131,1136.
[3]肖津安,白清玉,谢安明.早期糖尿病视网膜病变模型大鼠玻璃体腔注射安维汀后房水细胞因子变化[J].中国组织工程研究,2015,19(18):2933-2939.
[4]姚微微.抗CXCR4单克隆抗体对糖尿病大鼠血-视网膜屏障及视网膜血管内皮生长因子表达的影响[D].桂林:桂林医学院,2015.
[5]肖昂.雷珠单抗对糖尿病大鼠早期视网膜组织结构病变发生的影响[D].南昌:南昌大学,2015.
[6]董白霞,叶存喜,韩瑶,等.二十碳五烯酸对增殖性糖尿病性视网膜病变动物模型视网膜组织的超微结构改变的影响[J].河北医科大学学报,2015,36(6):677-681.
[7]李琴,杨安怀.玻璃体腔注射Lucentis联合治疗增殖性糖尿病性视网膜病变合并虹膜新生血管的临床观察[J].武汉大学学报(医学版),2016,37(6):998-1001,1017.
[8]刘青,艾明.雷珠单抗联合全视网膜光凝辅助玻璃体切割手术治疗糖尿病视网膜病变[J].国际眼科杂志,2016,16(9):1637-1640.
[9]李秀云.康柏西普联合低分子肝素辅助玻璃体切割术治疗PDR的长期疗效观察[D].济南:山东大学,2017.
[10]孙荻芳.糖尿病性视网膜病变玻璃体切除术后渗出性视网膜脱离相关因素分析[D].长春:吉林大学,2017.
[11]李康.脑红蛋白在增殖性糖尿病性视网膜病变患者眼部及血清中的表达及临床意义[D].太原:山西医科大学,2016.
[12]张招德.康柏西普辅助玻璃体手术治疗增殖性糖尿病性视网膜病变的临床疗效[J].中外医疗,2016,35(36):73-75.
[13]耿金,陈蕾.β-榄香烯抑制新生血管及在眼科疾病中的应用[J].解剖科学进展,2017,23(3):317-319.
[14]Pedinielli A,Bonnin S,Sanharawi ME,et al.Three different optical coherence tomography angiography measurement methods for assessing capillary density changes in diabetic retinopathy[J].Ophthalmic Surg Lasers Imaging Retina,2017,48(5):378-384.
[15]梁道桐,陈达钻,黄立,等.稳定控制血糖联合康柏西普玻璃体腔注射治疗增殖性糖尿病性视网膜病变临床观察[J].内科,2017,12(4):458-460.
[16]陆相庆.眼底激光治疗重度非增殖性糖尿病性视网膜病变疗效探讨[J].糖尿病新世界,2016,19(1):102-104.
[17]娄静,谢婷玉,陈雪艺.糖尿病大鼠眼内VEGF阶段性表达的Meta分析[J].国际眼科杂志,2015,15(10):1749-1752.