β-榄香烯对STZ致糖尿病性视网膜病变大鼠增殖期SIRT1和VEGF表达的影响
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摘要
目的研究β-榄香烯对链脲佐菌素(STZ)致糖尿病性视网膜病变大鼠增殖期沉默信息调节因子(SIRT1)和血管内皮生长因子(VEGF)表达的影响。方法选取80只Wistar大鼠作为研究对象,将其分为正常对照组、DR模型组、无菌柠檬酸组以及β-榄香烯组各20只。其中DR模型组、无菌柠檬酸组以及β-榄香烯组大鼠均经适应性喂养后通过腹腔一次性注射STZ制作糖尿病视网膜病变增殖期模型。无菌柠檬酸组大鼠造模成功后予以无菌柠檬酸腹腔注射干预,β-榄香烯组大鼠造模成功后则予以玻璃体腔内注射β-榄香烯干预。分别比较各组大鼠视网膜中SIRT1/β-actin相对灰度值、视网膜血管VEGF表达。结果 DR模型组、无菌柠檬酸组、β-榄香烯组以及正常对照组大鼠视网膜中SIRT1/β-actin相对灰度值分别为(1.218±0.033)、(1.305±0.052)、(1.411±0.088)、(1.502±0.103),呈逐渐升高趋势,且经单因素方差分析可得:组间比较差异均有统计学意义(P<0.05)。DR模型组、无菌柠檬酸组、β-榄香烯组以及正常对照组大鼠视网膜血管VEGF表达水平分别为(89.084±1.881)pg/m L、(57.920±0.910)pg/m L、(50.144±0.721)pg/m L、(44.382±0.661)pg/m L,呈逐渐下降趋势,且经单因素方差分析可得:组间比较差异均有统计学意义(P<0.05)。结论β-榄香烯对STZ致糖尿病性视网膜病变大鼠增殖期SIRT1表达具有一定的促进作用,而对VEGF表达具有明显的抑制作用。
Objective To study the effect of β-elemene on the expression of SIRT1 and VEGF in diabetic retinopathy rats induced by streptozotocin(STZ). Methods 80 Wistar rats were selected as study subjects and divided into normal control group, DR model group, sterile citric acid group and β-elemene group, with 20 cases in each group. Among them, DR model group, sterile citric acid group and β-elemene group were all fed with STZ to produce proliferative phase model of diabetic retinopathy. Sterile citric acid group was injected intraperitoneally into the rats after successful modeling, and β-elemene group was injected with β-elemene intravitreally after successful modeling. The relative gray levels of SIRT1/β-actin in the retina and the expression of VEGF in the retinal vessels were compared in each group.Results The relative gray values of SIRT1/β-actin in retinas of DR model group, sterile citric acid group, β-elemene group and normal control group were(1.218±0.033),(1.305±0.052), 1.411±0.088) and(1.502±0.103) respectively, showing a gradually increasing trend. There was a statistically significant difference between groups by one-way analysis of variance(P<0.05). The expression levels of VEGF in the retinal blood vessels of DR model group, sterile citric acid group,β-elemene group and normal control group were(89.084 ±1.881) pg/m L,(57.920 ±0.910)pg/m L,(50.144 ±0.721)pg/m L and(44.382±0.661)pg/m L respectively, showing a gradually decreasing trend. And there was a statistically significant difference between groups by one-way analysis of variance(P<0.05). Conclusion β-elemene has a certain role in promoting the expression of SIRT1 in rats with STZ-induced diabetic retinopathy and has a significant inhibitory effect on the expression of VEGF.
Objective To study the effect of β-elemene on the expression of SIRT1 and VEGF in diabetic retinopathy rats induced by streptozotocin(STZ). Methods 80 Wistar rats were selected as study subjects and divided into normal control group, DR model group, sterile citric acid group and β-elemene group, with 20 cases in each group. Among them, DR model group, sterile citric acid group and β-elemene group were all fed with STZ to produce proliferative phase model of diabetic retinopathy. Sterile citric acid group was injected intraperitoneally into the rats after successful modeling, and β-elemene group was injected with β-elemene intravitreally after successful modeling. The relative gray levels of SIRT1/β-actin in the retina and the expression of VEGF in the retinal vessels were compared in each group.Results The relative gray values of SIRT1/β-actin in retinas of DR model group, sterile citric acid group, β-elemene group and normal control group were(1.218±0.033),(1.305±0.052), 1.411±0.088) and(1.502±0.103) respectively, showing a gradually increasing trend. There was a statistically significant difference between groups by one-way analysis of variance(P<0.05). The expression levels of VEGF in the retinal blood vessels of DR model group, sterile citric acid group,β-elemene group and normal control group were(89.084 ±1.881) pg/m L,(57.920 ±0.910)pg/m L,(50.144 ±0.721)pg/m L and(44.382±0.661)pg/m L respectively, showing a gradually decreasing trend. And there was a statistically significant difference between groups by one-way analysis of variance(P<0.05). Conclusion β-elemene has a certain role in promoting the expression of SIRT1 in rats with STZ-induced diabetic retinopathy and has a significant inhibitory effect on the expression of VEGF.
引文
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[14]朴天华,帅天姣,杨岚.糖尿病性视网膜病变的发生机制及研究进展[J].牡丹江医学院学报,2015,35(3):102-104.
[15]郭建忠,魏尧,谷如雷,等.β-榄香烯诱导血管内皮细胞凋亡的研究[J].中国药物与临床,2016,16(8):1127-1129.
[2]詹琼,周鑫莉,黄若凡,等.β-榄香烯血药浓度测定方法的建立及其药动学研究[J].中国药房,2017,28(2):173-177.
[3]党晓敏,尚东,杜娟,等.榄香烯乳联合化疗对小鼠Lewis肺癌组织S100A4及MMP-9蛋白表达的影响[J].广州中医药大学学报,2017,34(6):897-902.
[4]赵艳霞,王爽.PDR抗VEGF药物眼内注射后玻璃体切割术的临床观察[J].航空航天医学杂志,2017,28(1):4-7.
[5]赵中敏,秦莹,范萍萍,等.糖尿病性视网膜病变患者眼局部注射糖皮质激素对血糖影响的研究[J].中国现代药物应用,2017,11(16):119-120.
[6]张久丹,郑杨,陈晔,等.维生素D缺乏与年轻1型糖尿病患者视网膜病变相关性的临床研究[J].中国现代医生,2017,55(21):17-20.
[7]梁道桐,陈达钻,黄立,等.稳定控制血糖联合康柏西普玻璃体腔注射治疗增殖性糖尿病性视网膜病变临床观察[J].内科,2017,12(4):458-460.
[8]邱贵娟,张韶君.维生素D与糖尿病视网膜病变关系的研究进展[J].中国现代医生,2016,54(20):158-161.
[9]周迪军,孙文利,双振宇,等.2型糖尿病并发视网膜病变的多重危险因素探讨[J].中国医药导报,2014,11(13):55-58.
[10]周赟,刘宁宁,万超,等.β-榄香烯对糖尿病大鼠视网膜中肿瘤坏死因子-α表达的影响[J].眼科新进展,2015,35(12):1129-1131.
[11]耿金,陈蕾,柳力敏,等.β榄香烯抑制氧诱导小鼠视网膜新生血管形成的实验研究[J].眼科新进展,2013,33(3):215-219.
[12]张笃贞,张涤,孙鹏,等.白藜芦醇对糖尿病大鼠视网膜中SIRT1基因表达的干预作用[J].眼科新进展,2014,34(4):322-325.
[13]王司仪,孙方格,刘早霞.家族性渗出性玻璃体视网膜病变合并视网膜脱离一例[J].新医学,2017,48(12):900-903.
[14]朴天华,帅天姣,杨岚.糖尿病性视网膜病变的发生机制及研究进展[J].牡丹江医学院学报,2015,35(3):102-104.
[15]郭建忠,魏尧,谷如雷,等.β-榄香烯诱导血管内皮细胞凋亡的研究[J].中国药物与临床,2016,16(8):1127-1129.