七氟醚对大鼠海马组织NR2B、Caspase-3的影响
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摘要
目的探讨七氟醚对大鼠海马组织N-甲基-D-天冬氨酸-2B受体(NR2B)、半胱氨酸蛋白酶3(Caspase-3)的影响。方法选取7d龄SD大鼠60只分为对照组和麻醉组,每组再分为3个时间点,即麻醉前、麻醉后24h及麻醉后7周,每组10只。麻醉组给予2.5%七氟醚麻醉4h,对照组仅给予空氧混合气。采用Western blot检测各组大鼠海马组织NR2B、Caspase-3蛋白表达,Morris水迷宫检测麻醉后7周大鼠的空间学习记忆能力。结果麻醉后24h,麻醉组大鼠海马组织NR2B、Caspase-3蛋白表达明显高于对照组及麻醉前(P<0.05);麻醉前及麻醉后7周,麻醉组和对照组大鼠海马组织NR2B、Caspase-3蛋白表达比较差异无统计学意义(P>0.05)。麻醉组大鼠第1、3天游泳潜伏期分别为(70.23±8.10)s和(41.03±7.39)s,总路程分别为(18.20±2.02)cm和(11.03±1.98)cm,明显高于对照组(P<0.05);麻醉组和对照组大鼠游泳速度比较差异无统计学意义(P>0.05)。结论七氟醚麻醉可即刻造成幼鼠海马组织NR2B、Caspase-3蛋白表达升高,同时其成年后的学习记忆能力有一定损伤,但成年后海马组织NR2B、Caspase-3蛋白表达无明显异常。
Objective To investigate the effect of sevoflurane on NR2B and Caspase-3 in rat hippocampal tissue.Methods Sixty SD rats of 7 dold were selected and divided into the control group and the anesthesia group,then each group was re-divided into 3 subgroups according to 3 timepoints,before anesthesia,24 h and 7 weeks after anesthesia,with 10 cases in each group.The anesthesia group was given 2.5% sevoflurane for 4 h,and the control group was given air oxygen mixture only.The expression of NR2B and Caspase-3 protein in hippocampal tissue of each group was detected by Western blot,and the rat spatial learning and memory ability in 7 weeks after anesthesia were detected by Morris water maze.Results The expression levels of NR2B protein and Caspase-3 protein in the hippocampus tissue at 24 hafter anesthesia in the anesthesia group were were significantly higher than those in the control group and before anesthesia(P<0.05).The expression levels of NR2B protein and Caspase-3 protein before anesthesia and at 7 weeks after anesthesia had no statistically significant difference between the anesthesia group and the control group(P>0.05).The swimming latency periods at 1,3 din the anesthesia group were(70.23±8.10)s and(41.03±7.39)s,and the total journeys were(18.20±2.02)cm and(11.03±1.98)cm respectively,which were significantly higher than those in the control group(P<0.05).There was no statistically significant difference in swimming speed between the anaesthesia group and the control group(P>0.05).Conclusion Sevoflurane anesthesia can instantly cause the increase of NR2B and Caspase-3 protein in the hippocampal tissue of young rats,meanwhile the learning and memory ability after adult has a certain damage,however,there is no obvious abnormality in hippocampus tissue NR2B and Caspase-3 protein expression after adult.
Objective To investigate the effect of sevoflurane on NR2B and Caspase-3 in rat hippocampal tissue.Methods Sixty SD rats of 7 dold were selected and divided into the control group and the anesthesia group,then each group was re-divided into 3 subgroups according to 3 timepoints,before anesthesia,24 h and 7 weeks after anesthesia,with 10 cases in each group.The anesthesia group was given 2.5% sevoflurane for 4 h,and the control group was given air oxygen mixture only.The expression of NR2B and Caspase-3 protein in hippocampal tissue of each group was detected by Western blot,and the rat spatial learning and memory ability in 7 weeks after anesthesia were detected by Morris water maze.Results The expression levels of NR2B protein and Caspase-3 protein in the hippocampus tissue at 24 hafter anesthesia in the anesthesia group were were significantly higher than those in the control group and before anesthesia(P<0.05).The expression levels of NR2B protein and Caspase-3 protein before anesthesia and at 7 weeks after anesthesia had no statistically significant difference between the anesthesia group and the control group(P>0.05).The swimming latency periods at 1,3 din the anesthesia group were(70.23±8.10)s and(41.03±7.39)s,and the total journeys were(18.20±2.02)cm and(11.03±1.98)cm respectively,which were significantly higher than those in the control group(P<0.05).There was no statistically significant difference in swimming speed between the anaesthesia group and the control group(P>0.05).Conclusion Sevoflurane anesthesia can instantly cause the increase of NR2B and Caspase-3 protein in the hippocampal tissue of young rats,meanwhile the learning and memory ability after adult has a certain damage,however,there is no obvious abnormality in hippocampus tissue NR2B and Caspase-3 protein expression after adult.
引文
[1]滕清宇,李玮伟,袁红斌,等.全麻药发育期神经毒性机制的研究进展[J].临床麻醉学杂志,2016,32(9):929-931.
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[9]田海涛,田苹,张宝娟,等.艾芬地尔预处理对七氟醚导致的幼年大鼠学习记忆和认知能力损伤的保护作用及机制[J].临床麻醉学杂志,2017,33(7):705-707.
[10]李珊,胡喆,周燕,等.趋化因子MCP-1对大鼠海马区NMDA受体介导的兴奋性突触后电流的影响[J].中国药理学通报,2016,32(7):950-955.
[11]DOLKA I,KROL M,SAPIERZYNSKI R.Evaluation of apoptosis-associated protein(Bcl-2,Bax,cleaved caspase-3and p53)expression in canine mammary tumors:an immunohistochemical and prognostic study[J].Res Vet Sci,2016,105(2):124-133.
[12]FLANAGAN L,MEYER M,FAY J,et al.Low levels of Caspase-3 predict favourable response to 5FU-based chemotherapy in advanced colorectal cancer:Caspase-3inhibition as a therapeutic approach[J].Cell Death Dis,2016,7(2):e2087.
[13]SáNCHEZ-MIGALLóN M C,VALIENTE-SORIANO F J,NADAL-NICOLáS F M,et al.Apoptotic retinal ganglion cell death after optic nerve transection or crush in mice:delayed RGC loss with BDNF or a caspase 3inhibitor[J].Invest Ophthalmol Vis Sci,2016,57(1):81-93.
[14]任娟娟,朱昭琼,王义,等.新生期重复吸入七氟醚的大鼠幼年期学习记忆功能与海马体积变化[J].医学研究生学报,2016,29(5):470-474.
[15]王权,张超,王义,等.新生期间断重复吸入七氟醚对大鼠学习记忆及Tau蛋白和p-Tau蛋白的影响[J].医学研究生学报,2017,30(3):257-261.
[2]李建立,侯艳宁.全身麻醉药对发育期大脑影响的研究进展[J].临床麻醉学杂志,2016,32(6):610-613.
[3]张广芬,杨建军.NMDA受体相关抗抑郁药物的研究进展[J].中国药理学通报,2018,34(1):1-4.
[4]黄科昌,楚云超,郑观荣,等.caspase-3在多塞平诱导大鼠神经细胞凋亡中的作用[J].中华麻醉学杂志,2016,36(1):46-48.
[5]孙越,孙越,孙满意,等.异氟醚对发育期大鼠海马神经干细胞p-GSK-3β和β-catenin表达的影响[J].中华麻醉学杂志,2017,37(1):66-69.
[6]闵娜,胡强夫,李晓培,等.异氟醚对新生大鼠海马神经干细胞增殖及分化的影响[J].中国组织工程研究,2016,20(1):118-122.
[7]谢思宁,叶虹,李俊发,等.七氟醚对幼鼠海马组织神经元凋亡和γ-氨基丁酸A受体α1/α2亚型组成及远期空间探索能力的影响[J].国际麻醉学与复苏杂志,2016,37(2):126-131.
[8]CIECHANOWICZ S,ZHAO H,CHEN Q,et al.Differential effects of sevoflurane on the metastatic potential and chemosensitivity of non-small-cell lung adenocarcinoma and renal cell carcinoma in vitro[J].Br J Anaesth,2018,120(2):368-375.
[9]田海涛,田苹,张宝娟,等.艾芬地尔预处理对七氟醚导致的幼年大鼠学习记忆和认知能力损伤的保护作用及机制[J].临床麻醉学杂志,2017,33(7):705-707.
[10]李珊,胡喆,周燕,等.趋化因子MCP-1对大鼠海马区NMDA受体介导的兴奋性突触后电流的影响[J].中国药理学通报,2016,32(7):950-955.
[11]DOLKA I,KROL M,SAPIERZYNSKI R.Evaluation of apoptosis-associated protein(Bcl-2,Bax,cleaved caspase-3and p53)expression in canine mammary tumors:an immunohistochemical and prognostic study[J].Res Vet Sci,2016,105(2):124-133.
[12]FLANAGAN L,MEYER M,FAY J,et al.Low levels of Caspase-3 predict favourable response to 5FU-based chemotherapy in advanced colorectal cancer:Caspase-3inhibition as a therapeutic approach[J].Cell Death Dis,2016,7(2):e2087.
[13]SáNCHEZ-MIGALLóN M C,VALIENTE-SORIANO F J,NADAL-NICOLáS F M,et al.Apoptotic retinal ganglion cell death after optic nerve transection or crush in mice:delayed RGC loss with BDNF or a caspase 3inhibitor[J].Invest Ophthalmol Vis Sci,2016,57(1):81-93.
[14]任娟娟,朱昭琼,王义,等.新生期重复吸入七氟醚的大鼠幼年期学习记忆功能与海马体积变化[J].医学研究生学报,2016,29(5):470-474.
[15]王权,张超,王义,等.新生期间断重复吸入七氟醚对大鼠学习记忆及Tau蛋白和p-Tau蛋白的影响[J].医学研究生学报,2017,30(3):257-261.