玉米MS22基因生物信息与GRX基因进化分析
|
摘要
谷氧还蛋白(glutaredoxin,GRX)是一类小分子氧化还原酶,在植物生长发育调控及逆境胁迫中起着重要调节作用。本研究拟对玉米MS22基因的生物信息及玉米中GRX基因家族的亲缘进化进行分析研究。通过对MS22基因的生物信息分析,发现MS22基因全长881 bp,编码159个氨基酸,属于CC型谷氧还蛋白。玉米基因组中共鉴定出22个GRX类基因,其中有6个基因为GRX-subⅠ,7个基因属于GRX-subⅡ,9个基因属于GRX-subⅢ。通过聚类分析发现MS22属于GRX-subⅢ类型,该蛋白与不同来源CC型谷氧还蛋白的氨基酸序列保守性较高,一致率介于61%~87%之间。通过该研究对玉米中该类型基因的进一步研究具有较好的理论指导意义。
Glutaredoxin(GRX) is a class of small molecule oxidoreductase, which plays an important role in regulating plant development and against abiotic stress. In this study, we analyzed the biological information of MS22 gene and the genetic evolution of GRX gene family in maize. Through analysis, we found that MS22 had 881 bp length of DNA, encoding 159 amino acids, which belonged to CC type glutaredoxin. 22 GRX genes were identified from maize, including 6 GRX-subⅠ genes, 7 GRX-sub Ⅱ genes, and 9 GRX-sub Ⅲ genes. Clustering analysis indicated that MS22 belonged to the GRX-sub Ⅲ type. This protein had high conservatism with the amino acid sequence of CC type glutaredoxin with different sources, and their concordance rate was between 61% and 87%. This study could provide better theoretical reference for further research of this type of genes in maize.
Glutaredoxin(GRX) is a class of small molecule oxidoreductase, which plays an important role in regulating plant development and against abiotic stress. In this study, we analyzed the biological information of MS22 gene and the genetic evolution of GRX gene family in maize. Through analysis, we found that MS22 had 881 bp length of DNA, encoding 159 amino acids, which belonged to CC type glutaredoxin. 22 GRX genes were identified from maize, including 6 GRX-subⅠ genes, 7 GRX-sub Ⅱ genes, and 9 GRX-sub Ⅲ genes. Clustering analysis indicated that MS22 belonged to the GRX-sub Ⅲ type. This protein had high conservatism with the amino acid sequence of CC type glutaredoxin with different sources, and their concordance rate was between 61% and 87%. This study could provide better theoretical reference for further research of this type of genes in maize.
引文
Akter S.,Huang J.J.,Waszczak C.,and Jacques S.,2015,Cysteines under ROS attack in plants:a proteomics view,JExp.Bot.,66(10):2935-2944
Chen Y.K.,Zhao Q.H.,Liu H.Z.,Zhou T.X.,and Xu J.C.,2016Cloning and bioinformatics analysis of glutaredoxin gene Pa GRXC13 in Phytolacca americana,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(8):2019-2024(陈永坤,赵启红,刘海珍,周天行,徐吉臣,2016,美洲商陆谷氧还蛋白基因Pa GRXC13的克隆与生物信息学分析,分子植物育种,14(8):2019-2024)
Guo Y.S.,Huang C.J.,Xie Y.,Song F.M.,and Zhou X.P.,2010Tomato glutaredoxin gene Sl GRX1 regulates plant responses to oxidative,drought and salt stresses,Planta,232(6):1499-1509
Guo Y.S.,Yu J.,Pu W.,Zou J.,Fu Q.,Yu S.Z.,Lin S.F.,Zhang X.L.,Zhao J.H.,and Xia F.J.,2017,Subcellular localization of Nb GRX1 and its role in plant response to drought stress Nanjing Nongye Daxue Xuebao(Journal of Nanjing Agric ultural University),40(1):34-39(郭玉双,余婧,蒲伟,邹颉,付强,余世洲,林世锋,张孝廉,赵杰宏,夏范讲,2017,烟草谷氧还蛋白Nb GRX1的亚细胞定位及抗旱功能研究,南京农业大学学报,40(1):34-39)
Hong L.L.,Tang D.,Zhu K.M.,Wang K.J.,Li M.,and Cheng Z.K.,2012,Somatic and reproductive cell development in rice anther is regulated by a putative glutaredoxin,Plant Cell,24(2):577-588
Hu Y.,Wu Q.Y.,Sprague S.A.,Park J.,Oh M.,Rajashekar C.B.,Koiwa H.,Nakata P.A.,Cheng N.H.,Hirschi K.D.,White F.F.,and Park S.H.,2015,Tomato expressing Arabidopsis glutaredoxin gene At GRXS17 confers tolerance to chilling stress via modulating cold responsive components,Hortic.Res.,2:15051
Huang G.,Wang X.,Jin X.F.,Wang X.J.,and Wang Y.Q.,2017,GRXC9 negatively regulates leaf size in Arabidopsis,Zhiwu Xuebao(Chinese Bulletin of Botany),52(5):550-559(黄淦,王潇,金学锋,王小菁,王亚琴,2017,拟南芥谷氧还蛋白GRXC9负调控叶片大小,植物学报,52(5):550-559)
Lee Y.K.,Kim G.T.,Kim I.J.,Park J.,Kwak S.S.,Choi G.,and Chung W.I.,2006,LONGIFOLIA1 and LONGIFOLIA2,two homologous genes,regulate longitudinal cell elongation in Arabidopsis,Development,133(21):4305-4314
Pham K.,Pal R.,Qu Y.,Liu X.,Yu H.,Shiao S.L.,Wang X.Q.,Smith E.O.,Cui X.J.,Rodney G.G.,and Cheng N.H.,2015,Nuclear glutaredoxin 3 is critical for protection against oxidative stress-induced cell death,Free Radical Biology and Medicine,85:197-206
Reczek C.R.,and Chandel N.S.,2015,ROS-dependent signal transduction,Curr.Opin.Cell Biol.,33:8-13
Rouhier N.,Gelhaye E.,and Jacquot J.P.,2004,Plant glutaredoxins:still mysterious reducing systems,Cellular and Molecular Life Sciences,61(11):1266-1277
Sharma R.,Priya P.,and Jain M.,2013,Modified expression of an auxin-responsive rice CC-type glutaredoxin gene affects multiple abiotic stress responses,Planta,238(5):871-884
Shelton M.D.,Chock P.B.,and Mieyal J.J.,2005,Glutaredoxin:role in reversible protein s-glutathionylation and regulation of redox signal transduction and protein translocation,Antioxidants&Redox Signaling,7(3-4):348-366
Sundaram S.,Wu S.,Ma L.Q.,and Rathinasabapathi B.,2009,Expression of a Pteris vittata glutaredoxin Pv GRX5 in transgenic Arabidopsis thaliana increases plant arsenic tolerance and decreases arsenic accumulation in the leaves,Plant Cell&Environment,32(7):851-858
Tian Y.,Zhang C.X.,Kang G.D.,Li W.X.,Zhang L.Y.,and Cong P.H.,2016,Progress on TGA transcription factors in plant,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),49(4):632-642(田义,张彩霞,康国栋,李武兴,张利益,丛佩华,2016,植物TGA转录因子研究进展,中国农业科学49(4):632-642)
Wang X.,Ruan M.B.,Wang B.,Guo X.,Yu X.L.,and Peng M.2017,Screening and expression analysis in vitro of CC-type glutaredoxin genes of cassava in response to drought stress Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(4):1204-1211(王雪,阮孟斌,王斌,郭鑫,于晓玲,彭明,2017木薯干旱胁迫响应CC类谷氧还蛋白基因的筛选与体外表达分析,分子植物育种,15(4):1204-1211)
Wang Y.,Liu G.J.,Yan X.F.,Yang C.P.,Liu M.K.,and Qu C.P.2010,Cloning of Ps PIP1 gene from Polygonum sibiricum Laxm and analysis of its expression in response to Na HCO3Yichuan(Hereditas),30(12):1621-1628(王垠,刘关君,阎秀峰,杨传平,刘明坤,曲春浦,2010,西伯利亚蓼Ps PIP1基因的克隆及其在Na HCO3胁迫下的表达,遗传,30(12)1621-1628)
Wang Z.,Xing S.P.,Birkenbihl R.P.,and Zachgo S.,2009,Conserved functions of Arabidopsis and rice CC-type glutare doxins in flower development and pathogen response,Molecular Plant,2(2):323-335
Wu Q.Y.,Lin J.,Liu J.Z.,Wang X.F.,Lim W.S.,Oh M.,Park J.,Rajashekar C.B.,Whitham S.A.,Cheng N.H.,Hirschi K.D.,and Park S.,2012,Ectopic expression of Arabidopsis glutaredoxin At GRXS17 enhances thermo tolerance in tomato,Plant Biotechnology Journal,10(8):945-955
Yang R.,Zhao Y.X.,and Bai W.,2015,Construction of RNA interference vector for CC-type glutaredoxin Os GRX6 in rice,Neimonggu Nongye Daxue Xuebao(Journal of Inner Mongolia Agricultural University(Natural Science Edition)),36(1):68-72(杨瑞,赵永秀,白薇,2015,水稻CC型谷氧还蛋白Os GRX6 RNAi干扰载体的构建,内蒙古农业大学学报(自然科学版),36(1):68-72)
Zhang H.H.,Du Y.T.,Zhang X.,Lu J.,and Holmgren A.,2014,Glutaredoxin 2 reduces both thioredoxin 2 and thioredoxin1and protects cells from apoptosis induced by auranofin and4-hydroxynonenal,Antioxidants&Redox Signaling,21(5):669-681
Chen Y.K.,Zhao Q.H.,Liu H.Z.,Zhou T.X.,and Xu J.C.,2016Cloning and bioinformatics analysis of glutaredoxin gene Pa GRXC13 in Phytolacca americana,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(8):2019-2024(陈永坤,赵启红,刘海珍,周天行,徐吉臣,2016,美洲商陆谷氧还蛋白基因Pa GRXC13的克隆与生物信息学分析,分子植物育种,14(8):2019-2024)
Guo Y.S.,Huang C.J.,Xie Y.,Song F.M.,and Zhou X.P.,2010Tomato glutaredoxin gene Sl GRX1 regulates plant responses to oxidative,drought and salt stresses,Planta,232(6):1499-1509
Guo Y.S.,Yu J.,Pu W.,Zou J.,Fu Q.,Yu S.Z.,Lin S.F.,Zhang X.L.,Zhao J.H.,and Xia F.J.,2017,Subcellular localization of Nb GRX1 and its role in plant response to drought stress Nanjing Nongye Daxue Xuebao(Journal of Nanjing Agric ultural University),40(1):34-39(郭玉双,余婧,蒲伟,邹颉,付强,余世洲,林世锋,张孝廉,赵杰宏,夏范讲,2017,烟草谷氧还蛋白Nb GRX1的亚细胞定位及抗旱功能研究,南京农业大学学报,40(1):34-39)
Hong L.L.,Tang D.,Zhu K.M.,Wang K.J.,Li M.,and Cheng Z.K.,2012,Somatic and reproductive cell development in rice anther is regulated by a putative glutaredoxin,Plant Cell,24(2):577-588
Hu Y.,Wu Q.Y.,Sprague S.A.,Park J.,Oh M.,Rajashekar C.B.,Koiwa H.,Nakata P.A.,Cheng N.H.,Hirschi K.D.,White F.F.,and Park S.H.,2015,Tomato expressing Arabidopsis glutaredoxin gene At GRXS17 confers tolerance to chilling stress via modulating cold responsive components,Hortic.Res.,2:15051
Huang G.,Wang X.,Jin X.F.,Wang X.J.,and Wang Y.Q.,2017,GRXC9 negatively regulates leaf size in Arabidopsis,Zhiwu Xuebao(Chinese Bulletin of Botany),52(5):550-559(黄淦,王潇,金学锋,王小菁,王亚琴,2017,拟南芥谷氧还蛋白GRXC9负调控叶片大小,植物学报,52(5):550-559)
Lee Y.K.,Kim G.T.,Kim I.J.,Park J.,Kwak S.S.,Choi G.,and Chung W.I.,2006,LONGIFOLIA1 and LONGIFOLIA2,two homologous genes,regulate longitudinal cell elongation in Arabidopsis,Development,133(21):4305-4314
Pham K.,Pal R.,Qu Y.,Liu X.,Yu H.,Shiao S.L.,Wang X.Q.,Smith E.O.,Cui X.J.,Rodney G.G.,and Cheng N.H.,2015,Nuclear glutaredoxin 3 is critical for protection against oxidative stress-induced cell death,Free Radical Biology and Medicine,85:197-206
Reczek C.R.,and Chandel N.S.,2015,ROS-dependent signal transduction,Curr.Opin.Cell Biol.,33:8-13
Rouhier N.,Gelhaye E.,and Jacquot J.P.,2004,Plant glutaredoxins:still mysterious reducing systems,Cellular and Molecular Life Sciences,61(11):1266-1277
Sharma R.,Priya P.,and Jain M.,2013,Modified expression of an auxin-responsive rice CC-type glutaredoxin gene affects multiple abiotic stress responses,Planta,238(5):871-884
Shelton M.D.,Chock P.B.,and Mieyal J.J.,2005,Glutaredoxin:role in reversible protein s-glutathionylation and regulation of redox signal transduction and protein translocation,Antioxidants&Redox Signaling,7(3-4):348-366
Sundaram S.,Wu S.,Ma L.Q.,and Rathinasabapathi B.,2009,Expression of a Pteris vittata glutaredoxin Pv GRX5 in transgenic Arabidopsis thaliana increases plant arsenic tolerance and decreases arsenic accumulation in the leaves,Plant Cell&Environment,32(7):851-858
Tian Y.,Zhang C.X.,Kang G.D.,Li W.X.,Zhang L.Y.,and Cong P.H.,2016,Progress on TGA transcription factors in plant,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),49(4):632-642(田义,张彩霞,康国栋,李武兴,张利益,丛佩华,2016,植物TGA转录因子研究进展,中国农业科学49(4):632-642)
Wang X.,Ruan M.B.,Wang B.,Guo X.,Yu X.L.,and Peng M.2017,Screening and expression analysis in vitro of CC-type glutaredoxin genes of cassava in response to drought stress Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(4):1204-1211(王雪,阮孟斌,王斌,郭鑫,于晓玲,彭明,2017木薯干旱胁迫响应CC类谷氧还蛋白基因的筛选与体外表达分析,分子植物育种,15(4):1204-1211)
Wang Y.,Liu G.J.,Yan X.F.,Yang C.P.,Liu M.K.,and Qu C.P.2010,Cloning of Ps PIP1 gene from Polygonum sibiricum Laxm and analysis of its expression in response to Na HCO3Yichuan(Hereditas),30(12):1621-1628(王垠,刘关君,阎秀峰,杨传平,刘明坤,曲春浦,2010,西伯利亚蓼Ps PIP1基因的克隆及其在Na HCO3胁迫下的表达,遗传,30(12)1621-1628)
Wang Z.,Xing S.P.,Birkenbihl R.P.,and Zachgo S.,2009,Conserved functions of Arabidopsis and rice CC-type glutare doxins in flower development and pathogen response,Molecular Plant,2(2):323-335
Wu Q.Y.,Lin J.,Liu J.Z.,Wang X.F.,Lim W.S.,Oh M.,Park J.,Rajashekar C.B.,Whitham S.A.,Cheng N.H.,Hirschi K.D.,and Park S.,2012,Ectopic expression of Arabidopsis glutaredoxin At GRXS17 enhances thermo tolerance in tomato,Plant Biotechnology Journal,10(8):945-955
Yang R.,Zhao Y.X.,and Bai W.,2015,Construction of RNA interference vector for CC-type glutaredoxin Os GRX6 in rice,Neimonggu Nongye Daxue Xuebao(Journal of Inner Mongolia Agricultural University(Natural Science Edition)),36(1):68-72(杨瑞,赵永秀,白薇,2015,水稻CC型谷氧还蛋白Os GRX6 RNAi干扰载体的构建,内蒙古农业大学学报(自然科学版),36(1):68-72)
Zhang H.H.,Du Y.T.,Zhang X.,Lu J.,and Holmgren A.,2014,Glutaredoxin 2 reduces both thioredoxin 2 and thioredoxin1and protects cells from apoptosis induced by auranofin and4-hydroxynonenal,Antioxidants&Redox Signaling,21(5):669-681