产抗菌肽芽孢杆菌的制剂制备及其对仔猪生产性能的影响
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摘要
本试验以产抗菌肽的枯草芽孢杆菌GL08为出发菌株,通过单因素和正交试验确定最佳的喷雾干燥工艺参数。并将制备的抗菌肽制剂作为饲料添加剂,验证其对仔猪生产性能的影响。优化后的喷雾干燥工艺为出风温度75℃,进风温度135℃,保护剂辅美粉浓度20%。在此工艺条件下,抗菌肽得率为91.6%,含水量为3.94%,符合抗菌肽制剂质量要求。此工艺生产成本低,效率高,适合工业大规模生产。制得的抗菌肽制剂作为饲料添加剂使仔猪的料肉比提高25%,残次率降低63%,成活率提高8.3%,无腹泻发生,提高了仔猪的生产性能和健康水平。
The optimum spray drying process parameters were obtained for the Bacillus subtilis GL08 of producing antibacterial peptide as starting strain by single factor and orthogonal test.And antibacterial peptide preparation was verified the effect on production performance of piglets as feed additives.The optimized parameters:outlet air temperature was 75 ℃,inlet air temperature was 135 ℃,protective agent concentration was 20%.under the process conditions,the yield of antibacterial peptide was 91.6%,the moisture content was 3.94%,which conformed to the requirements of the antimicrobial peptide preparation quality.The process was low cost,high efficiency and suitable for industrial mass production.The antibacterial peptide preparation could be used as feed additives in order to improve the piglet feed conversion ratio by 25%,reduce the defect rate by 63%,improve the survival rate by 8.3%,no diarrhea,and more,improve the production performance and health level of piglets.
The optimum spray drying process parameters were obtained for the Bacillus subtilis GL08 of producing antibacterial peptide as starting strain by single factor and orthogonal test.And antibacterial peptide preparation was verified the effect on production performance of piglets as feed additives.The optimized parameters:outlet air temperature was 75 ℃,inlet air temperature was 135 ℃,protective agent concentration was 20%.under the process conditions,the yield of antibacterial peptide was 91.6%,the moisture content was 3.94%,which conformed to the requirements of the antimicrobial peptide preparation quality.The process was low cost,high efficiency and suitable for industrial mass production.The antibacterial peptide preparation could be used as feed additives in order to improve the piglet feed conversion ratio by 25%,reduce the defect rate by 63%,improve the survival rate by 8.3%,no diarrhea,and more,improve the production performance and health level of piglets.
引文
[1]付博,马齐,王卫卫,等.真空冷冻干燥与喷雾干燥长双歧杆菌的工艺比较研究[J].食品科学,2012,33(7):188~192.
[2]黄乐天,曹丁,黄魁英,等.微囊化蜡样芽胞杆菌的喷雾干燥工艺优化[J].安徽农业科学,2014,42(20):6546~6548.
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[4]李明华,孟秀梅,徐生林,等.枯草芽孢杆菌微生态制剂的制备及其对雏鸡生产性能的影响[J].饲料研究,2014,11:1~4.
[5]田木.益生菌复合微生态制剂的制备工艺及免疫功能研究:[硕士学位论文][D].长春:吉林大学,2015.
[6]Cao D,Li H,Jiang Z,et al.Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of Cp G-ODN in newborn piglets[J].Vaccine,2010,28(37):6006~6013.
[7]Ganz T.Defensins:antimicrobial peptides of innate immunity[J].Nat Rev Immunol,2003,3:710~720.
[8]Gardiner G E,Sullivan E O,Kelly J,et al.Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L.salivarius strains during heat treatment and spray drying[J].Applied and Environmental Microbiology,2000,6:2605~2612.
[9]Nakatsuji T,Gallo R L.Antimicrobial peptides:old molecules with new ideas[J].J Invest Dermatol,2012,132(3 Pt 2):887~895.
[2]黄乐天,曹丁,黄魁英,等.微囊化蜡样芽胞杆菌的喷雾干燥工艺优化[J].安徽农业科学,2014,42(20):6546~6548.
[3]梁洁,彭中键,梁淑娃,等.转基因酵母菌深层发酵生产抗菌肽的研究[J].现代食品科技,2009,25(6):639~640.
[4]李明华,孟秀梅,徐生林,等.枯草芽孢杆菌微生态制剂的制备及其对雏鸡生产性能的影响[J].饲料研究,2014,11:1~4.
[5]田木.益生菌复合微生态制剂的制备工艺及免疫功能研究:[硕士学位论文][D].长春:吉林大学,2015.
[6]Cao D,Li H,Jiang Z,et al.Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of Cp G-ODN in newborn piglets[J].Vaccine,2010,28(37):6006~6013.
[7]Ganz T.Defensins:antimicrobial peptides of innate immunity[J].Nat Rev Immunol,2003,3:710~720.
[8]Gardiner G E,Sullivan E O,Kelly J,et al.Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L.salivarius strains during heat treatment and spray drying[J].Applied and Environmental Microbiology,2000,6:2605~2612.
[9]Nakatsuji T,Gallo R L.Antimicrobial peptides:old molecules with new ideas[J].J Invest Dermatol,2012,132(3 Pt 2):887~895.