马铃薯糖苷生物碱对化疗性静脉炎兔血清VEGF及IL-6表达的影响
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摘要
目的:探讨马铃薯糖苷生物碱(主要包括α-茄碱和α-卡茄碱)外敷防治兔耳缘静脉化疗性静脉炎的作用,以及对静脉局部血清血管内皮生长因子(Vascular endothelial growth factor,VEGF)及白细胞介素6 (Interleukin 6,IL-6)表达的影响。方法:将32只新西兰兔随机分成8组,除空白组(F组)外,其余各组建立长春新碱致兔耳缘静脉化疗性静脉炎模型。造模成功后药物外用干预,模型组(A组)用不含药霜膏涂抹,B组硫酸镁湿敷,C组新鲜马铃薯泥外敷,D1、D2组分别用0.5 g浓度为100 mg/kg及200 mg/kgα-茄碱霜膏外涂,E1、E2组用0.5 g浓度为100 mg/kg及200 mg/kgα-卡茄碱霜膏外涂。观察静脉炎发生情况,并取静脉穿刺点周围血清,ELISA法测定各组标本血清中的VEGF及IL-6表达水平。结果:与F组比较,A组兔耳静脉穿刺部位静脉炎症状明显,VEGF和IL-6表达水平远高于正常浓度(P <0.05);与A组比较,其余组兔耳静脉穿刺部位静脉炎症状均有不同程度的减轻,D2及E2组症状改善最为明显,静脉炎发生率降低,VEGF和IL-6表达水平显著降低(P <0.05)。结论:马铃薯糖苷生物碱外敷能明显改善长春新碱致兔耳缘静脉化疗性静脉炎组织发红、肿胀、疼痛、血管硬化等症状,能有效控制局部穿刺血清中VEGF和IL-6表达水平,且效果优于硫酸镁湿敷与新鲜马铃薯外敷。
Objective:To discuss the effect of the external application with potato glycoalkaloids,mainly including α-solanine andα-chaconine, on the prevention and treatment of chemotherapy-induced phlebitis in ear veins of rabbits, and on the expression of vascular endothelial growth factor(VEGF) and interleukin 6(IL-6) in local serum in veins. Methods:A total of 32 New Zealand rabbits were randomly divided into eight groups. The rabbit models with chemotherapy-induced phlebitis in ear veins induced by vincristine were established in each group except the blank group(Group F). After successful modeling, external intervention were performed in all groups. The model group(Group A) was smeared with cream without medicinal substance; Group B was given wet dressing with magnesium sulfate;Group C was treated with external application with freshly mashed potatoes;Group D1 and Group D2 were treated with external application with 0.5 g of 100 mg/kg and 200 mg/kg of α-solanine cream respectively;Group E1 and Group E2 were treated with external application with 0.5 g of 100 mg/kg and 200 mg/kg of α-chaconine cream respectively. The occurrence of phlebitis was observed and the serum around venipuncture sites was extracted. The levels of the expression of VEGF and IL-6 in serum sample in each group were measured by ELISA method. Results:The symptoms of phlebitis around venipuncture sites of rabbit ears in Group A were more significant than those in Group F,and the levels of the expression of VEGF and IL-6 in Group A were much higher than normal concentration(P < 0.05). Compared with those in Group A,the symptoms of phlebitis around venipuncture sites of rabbit ears in the other groups were alleviated in different degrees; in Group D2 and Group E2,the improvement of symptoms was most significant,the incidence of phlebitis was decreased, and levels of the expression of VEGF and IL-6 were significantly decreased(P < 0.05).Conclusion:The external application with potato glycoalkaloids can significantly improve the symptoms of chemotherapy-induced phlebitis in ear veins of rabbit models induced by vincristine,including redness,swelling,pain and vascular sclerosis,and effectively control the levels of the expression of VEGF and IL-6 in serum in local puncture sites,which has better effect than wet dressing of magnesium sulfate and external application with freshly mashed potatoes.
Objective:To discuss the effect of the external application with potato glycoalkaloids,mainly including α-solanine andα-chaconine, on the prevention and treatment of chemotherapy-induced phlebitis in ear veins of rabbits, and on the expression of vascular endothelial growth factor(VEGF) and interleukin 6(IL-6) in local serum in veins. Methods:A total of 32 New Zealand rabbits were randomly divided into eight groups. The rabbit models with chemotherapy-induced phlebitis in ear veins induced by vincristine were established in each group except the blank group(Group F). After successful modeling, external intervention were performed in all groups. The model group(Group A) was smeared with cream without medicinal substance; Group B was given wet dressing with magnesium sulfate;Group C was treated with external application with freshly mashed potatoes;Group D1 and Group D2 were treated with external application with 0.5 g of 100 mg/kg and 200 mg/kg of α-solanine cream respectively;Group E1 and Group E2 were treated with external application with 0.5 g of 100 mg/kg and 200 mg/kg of α-chaconine cream respectively. The occurrence of phlebitis was observed and the serum around venipuncture sites was extracted. The levels of the expression of VEGF and IL-6 in serum sample in each group were measured by ELISA method. Results:The symptoms of phlebitis around venipuncture sites of rabbit ears in Group A were more significant than those in Group F,and the levels of the expression of VEGF and IL-6 in Group A were much higher than normal concentration(P < 0.05). Compared with those in Group A,the symptoms of phlebitis around venipuncture sites of rabbit ears in the other groups were alleviated in different degrees; in Group D2 and Group E2,the improvement of symptoms was most significant,the incidence of phlebitis was decreased, and levels of the expression of VEGF and IL-6 were significantly decreased(P < 0.05).Conclusion:The external application with potato glycoalkaloids can significantly improve the symptoms of chemotherapy-induced phlebitis in ear veins of rabbit models induced by vincristine,including redness,swelling,pain and vascular sclerosis,and effectively control the levels of the expression of VEGF and IL-6 in serum in local puncture sites,which has better effect than wet dressing of magnesium sulfate and external application with freshly mashed potatoes.
引文
[1]靳英辉,赵晨,甘惠,等.化疗性静脉炎护理干预效果的网状Meta分析[J].护理学杂志,2016,31(4):85-90.
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[6]庞敏,王冬,李婷,等.重组大鼠CC16蛋白质对LPS诱导大鼠气道上皮细胞表达TNF-α、IL-6和IL-8的影响[J].中国病理生理杂志,2016,32(10):1843-1847.
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[9]薄丽丽,孙晓彦,林浩,等.马铃薯中糖苷生物碱的提取研究[J].安徽农业科学,2012,40(3):1448-1492.
[10]黄志宏,蒋东旭,谢友良,等.马铃薯中总糖苷生物碱的制备工艺优化及抗炎活性研究[J].中国药师,2016,19(10):1866-1869.
[11]申建宾.长春瑞滨在输液性静脉炎小鼠模型建立中的作用及干预措施[J].中国老年学杂志,2017,37(20):4980-4981.
[12]谢丽微,杜乐燕,苏银法,等.马铃薯外敷对长春瑞滨输液性静脉炎ICAM-1和VEGF表达的影响[J].温州医学院学报,2012,42(5):458-460.
[13]张秋实,张振香,杨亚平,等.马铃薯对甘露醇诱导静脉炎模型兔血清IL-6水平及局部VEGF表达的影响[J].郑州大学学报,2012,47(2):175-177.
[2]李芳.新鲜土豆片对化疗性静脉炎预防效果的临床观察[J].国际护理学杂志,2015,34(14):1997-1998.
[3]梁克红,卢林纲,朱大洲,等.马铃薯糖苷生物碱的研究进展[J].食品研究与开发,2017,38(21):195-199.
[4]王萍,金立秀,李海凤,等.PICC所致静脉炎的原因分析及护理干预[J].护士进修杂志,2015,30(1):75-76.
[5]黄利全,陈黎,毛宇飞,等.外源性碱性成纤维细胞生长因子对Ⅲ级静脉炎模型兔VEGF表达的影响[J].中国老年学杂志,2014,34(20):5821-5823.
[6]庞敏,王冬,李婷,等.重组大鼠CC16蛋白质对LPS诱导大鼠气道上皮细胞表达TNF-α、IL-6和IL-8的影响[J].中国病理生理杂志,2016,32(10):1843-1847.
[7]毛惠娜,刘雪琴.静脉输液渗出的发生率调查与分析[J].中国护理管理,2010,10(12):71-73.
[8]付晓燕,苏韫,颜春鲁,等.马铃薯糖苷生物碱减轻化疗性静脉炎作用机制的实验研究[J].护理学杂志,2018,33(1):31-33.
[9]薄丽丽,孙晓彦,林浩,等.马铃薯中糖苷生物碱的提取研究[J].安徽农业科学,2012,40(3):1448-1492.
[10]黄志宏,蒋东旭,谢友良,等.马铃薯中总糖苷生物碱的制备工艺优化及抗炎活性研究[J].中国药师,2016,19(10):1866-1869.
[11]申建宾.长春瑞滨在输液性静脉炎小鼠模型建立中的作用及干预措施[J].中国老年学杂志,2017,37(20):4980-4981.
[12]谢丽微,杜乐燕,苏银法,等.马铃薯外敷对长春瑞滨输液性静脉炎ICAM-1和VEGF表达的影响[J].温州医学院学报,2012,42(5):458-460.
[13]张秋实,张振香,杨亚平,等.马铃薯对甘露醇诱导静脉炎模型兔血清IL-6水平及局部VEGF表达的影响[J].郑州大学学报,2012,47(2):175-177.