干旱和复水对玉米幼苗叶绿素荧光参数的影响
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摘要
以玉米为实验材料,研究了干旱胁迫对玉米幼苗叶绿素荧光参数的影响.结果表明:与对照相比,干旱胁迫下,随着土壤含水量的降低,玉米叶片水势下降,叶片叶绿素荧光参数Fv、Fv/Fm、ΦPSⅡ和qP不断下降,下降程度随干旱胁迫加剧而逐渐增大;而qN则随干旱胁迫程度加剧而增加;且复水后,各参数均有明显恢复趋势,说明干旱造成的PSⅡ活性下调可能与光系统通过非光化学耗散过剩激发能以保护光合机构免于进一步破坏有关.
The method of chlorophyll fluorescence kinetics technology was used to study chlorophyll fluorescence parameters of maize seedling under drought stress. The results showed that under the drought condition,leaf water contents were decreased along with the decline in soil water contents. Chlorophyll fluorescence parameters derived from analyzing modulated chlorophyll fluorescence, such as such as Fv/Fm,Yeild and q P, showed a strong progressive drop on the days 3, 5, and 7 after water defitics application, showed a significant decreases in mize leaves in response to progressive water deficit. However,.q N showed a reverse trend. This implied that the declines in components of thylakoid membrane caused by water deficits and salinity might contribute to protect the photosynthetic apparatus against damage of excessive energy.
The method of chlorophyll fluorescence kinetics technology was used to study chlorophyll fluorescence parameters of maize seedling under drought stress. The results showed that under the drought condition,leaf water contents were decreased along with the decline in soil water contents. Chlorophyll fluorescence parameters derived from analyzing modulated chlorophyll fluorescence, such as such as Fv/Fm,Yeild and q P, showed a strong progressive drop on the days 3, 5, and 7 after water defitics application, showed a significant decreases in mize leaves in response to progressive water deficit. However,.q N showed a reverse trend. This implied that the declines in components of thylakoid membrane caused by water deficits and salinity might contribute to protect the photosynthetic apparatus against damage of excessive energy.
引文
[1]Massacci A,Nabiev SM,Pietrosanti L,Nematov SK,et al.Response of the photosynthetic apparatus of cotton(Gossypium hirsutum)to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging[J].Plant Physiology&Biochemistry,2008,46(2):189-195.
[2]赵丽英,邓西平,山仑.渗透胁迫对小麦幼苗叶绿素荧光参数的影响[J].应用生态学报,2005,16(7):126l-1264.
[3]Schreiber U,Bilger W,Neubauer C.Chlorophyll fluorescence as a non-intrusive indicator for rapid assessment of in vivo photosynthesis.In:Schulze D-E,Caldwell MM,editors.Ecophysiology of photosynthesis.Berlin:Springer-Verlag.1994.
[4]Santos M G,Ribeiro R V,Machado E C,Pimentel C.Photosynthetic parameters and leaf water potential of five common bean genotypes under mild water deficit[J].Biologia Plantarum,2009,53(2):229-236.
[5]Schreiber U,Gademann R,Ralph P J,Larkum A W D.Assessment of Photosynthetic Performance of Prochloron in Lissoclinum patella in hospite by Chlorophyll Fluorescence Measurements[J].Plant&Cell Physiology,1997,38(8):945-951.
[6]卢从明,张其德,匡廷云.水分胁迫对小麦叶绿素a荧光诱导动力学的影响[J].生物物理学报,1993,9(3):453-457.
[7]Baker N R.Chlorophyll fluorescence:a probe of photosynthesis in vivo[J].Annual Review of Plant Biology,2008,59(1):89-113.
[8]韩志国,雷腊梅,韩博平.利用调制荧光仪在线监测叶绿素荧光[J].生态科学,2005,24(3):246-249.
[9]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京.科学出版社,1999:p95.
[10]徐世昌,崔钦.水分胁迫对玉米光合性能及产量的影响[J].作物学报,1995,21(3):356-363.
[11]Genty B,Briantais JM,Baker NR.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochim Biophys Acta,1989,990:87-92.
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[13]Van Kooten O,Snel J F H.The use of chlorophyll fluorescence nomenclature in pla nt stress physiology[J].Photosynthesis Research,1990,25:147-150.
[14]王可玢,许春辉,赵福洪,唐崇钦,戴云玲.水分胁迫对小麦旗叶某些体内叶绿素a荧光参数的影响[J].生物物理学报,1997,13(2):273-278.
[15]张守仁.叶绿素荧光动力学参数及其意义的讨论[J].植物学通报,1999,16(4):444-448.
[16]李志博,魏亦农,张荣华,张小均.棉花不同叶位叶绿素荧光特性的初步研究[J.棉花学报,2005,17(3):189-190.
[17]Ogren E,Oquist G.Photoinhibition of photosynthesis in Lewma qib ba as induced by the interaction between light and temperatureⅢ:Chloroph yll fluorescence at 77K[J].Physiol Plant,1984,62:193-200.
[18]Demmig B,Bjorkman O.Comparison of the effect of excessive light on chlorophyll fluorescence(77K)and photon yield of O2 evolution of leaves of higher plants[J].Planta,1987,171:171-184.
[19]罗俊,张木清,吕建林.水分胁迫对不同甘蔗品种叶绿素a荧光动力学的影响[J].福建农业大学学报,2000,(1):18-22.
[20]Demmig-Adams B,AdamsⅢWW,Barker DH.Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation[J].Physiol Plant,1996,98:253-264.
[21]徐建伟,席万鹏,方憬军,亚合甫·木沙.水分胁迫对葡萄叶绿素荧光参数的影响西北农业学报[J].2007,16(5):175-179.
[22]张秋英,李发东,刘孟雨,等.水分胁迫对冬小麦旗叶光合作用的影响[J].干旱地区农业研究,2002,20(3):80-84.
[23]关军峰.果品品质研究[M].石家庄:河北科学技术出版社,2001:105-116.
[24]陈军,戴俊英.干旱对不同耐性玉米品种光合作用及产量的影响[J].作物学报,1996,22(6):757-762.
[25]Baker N R.A possible role for photosystemⅡin environmental pertubations of photosynthesis[J].Physiol Plant,1991,81:563-570.
[26]Demmig-Adams B,AdamsⅢWW.Photoprotection and other responses of plants to high light stress[J].Annu Rev Plant Physiol Plant Mol Biol,1992,43:559-626.
[27]许大权.光系统Ⅱ反应中心的可逆失活及其生理意义[J].植物生理通讯,1999,35(4):273-276.
[28]Wollman F A,Minai L,Nechushtai R.The biogenesis and assembly of photosynthetic proteins in thylakoid membranes1[J].Biochim Biophys Acta,1999,1411:21-85.
[2]赵丽英,邓西平,山仑.渗透胁迫对小麦幼苗叶绿素荧光参数的影响[J].应用生态学报,2005,16(7):126l-1264.
[3]Schreiber U,Bilger W,Neubauer C.Chlorophyll fluorescence as a non-intrusive indicator for rapid assessment of in vivo photosynthesis.In:Schulze D-E,Caldwell MM,editors.Ecophysiology of photosynthesis.Berlin:Springer-Verlag.1994.
[4]Santos M G,Ribeiro R V,Machado E C,Pimentel C.Photosynthetic parameters and leaf water potential of five common bean genotypes under mild water deficit[J].Biologia Plantarum,2009,53(2):229-236.
[5]Schreiber U,Gademann R,Ralph P J,Larkum A W D.Assessment of Photosynthetic Performance of Prochloron in Lissoclinum patella in hospite by Chlorophyll Fluorescence Measurements[J].Plant&Cell Physiology,1997,38(8):945-951.
[6]卢从明,张其德,匡廷云.水分胁迫对小麦叶绿素a荧光诱导动力学的影响[J].生物物理学报,1993,9(3):453-457.
[7]Baker N R.Chlorophyll fluorescence:a probe of photosynthesis in vivo[J].Annual Review of Plant Biology,2008,59(1):89-113.
[8]韩志国,雷腊梅,韩博平.利用调制荧光仪在线监测叶绿素荧光[J].生态科学,2005,24(3):246-249.
[9]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京.科学出版社,1999:p95.
[10]徐世昌,崔钦.水分胁迫对玉米光合性能及产量的影响[J].作物学报,1995,21(3):356-363.
[11]Genty B,Briantais JM,Baker NR.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochim Biophys Acta,1989,990:87-92.
[12]Schreiber U,Bilger W,Neubauer G.In:Ecophysiology of Photosynthesis[M].(eds Schulze,E-D and Caldwell,M M.),Springer-Verlag,Berlin,1994.
[13]Van Kooten O,Snel J F H.The use of chlorophyll fluorescence nomenclature in pla nt stress physiology[J].Photosynthesis Research,1990,25:147-150.
[14]王可玢,许春辉,赵福洪,唐崇钦,戴云玲.水分胁迫对小麦旗叶某些体内叶绿素a荧光参数的影响[J].生物物理学报,1997,13(2):273-278.
[15]张守仁.叶绿素荧光动力学参数及其意义的讨论[J].植物学通报,1999,16(4):444-448.
[16]李志博,魏亦农,张荣华,张小均.棉花不同叶位叶绿素荧光特性的初步研究[J.棉花学报,2005,17(3):189-190.
[17]Ogren E,Oquist G.Photoinhibition of photosynthesis in Lewma qib ba as induced by the interaction between light and temperatureⅢ:Chloroph yll fluorescence at 77K[J].Physiol Plant,1984,62:193-200.
[18]Demmig B,Bjorkman O.Comparison of the effect of excessive light on chlorophyll fluorescence(77K)and photon yield of O2 evolution of leaves of higher plants[J].Planta,1987,171:171-184.
[19]罗俊,张木清,吕建林.水分胁迫对不同甘蔗品种叶绿素a荧光动力学的影响[J].福建农业大学学报,2000,(1):18-22.
[20]Demmig-Adams B,AdamsⅢWW,Barker DH.Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation[J].Physiol Plant,1996,98:253-264.
[21]徐建伟,席万鹏,方憬军,亚合甫·木沙.水分胁迫对葡萄叶绿素荧光参数的影响西北农业学报[J].2007,16(5):175-179.
[22]张秋英,李发东,刘孟雨,等.水分胁迫对冬小麦旗叶光合作用的影响[J].干旱地区农业研究,2002,20(3):80-84.
[23]关军峰.果品品质研究[M].石家庄:河北科学技术出版社,2001:105-116.
[24]陈军,戴俊英.干旱对不同耐性玉米品种光合作用及产量的影响[J].作物学报,1996,22(6):757-762.
[25]Baker N R.A possible role for photosystemⅡin environmental pertubations of photosynthesis[J].Physiol Plant,1991,81:563-570.
[26]Demmig-Adams B,AdamsⅢWW.Photoprotection and other responses of plants to high light stress[J].Annu Rev Plant Physiol Plant Mol Biol,1992,43:559-626.
[27]许大权.光系统Ⅱ反应中心的可逆失活及其生理意义[J].植物生理通讯,1999,35(4):273-276.
[28]Wollman F A,Minai L,Nechushtai R.The biogenesis and assembly of photosynthetic proteins in thylakoid membranes1[J].Biochim Biophys Acta,1999,1411:21-85.