子痫前期孕妇胎盘组织中miR-205表达的研究
|
摘要
目的检测子痫前期孕妇胎盘组织中miR-205表达,并探讨其在子痫前期致病中的作用。方法 2017年10月至2018年7月,在东莞市人民医院就诊的子痫前期孕妇中,选择30例纳入本研究,选择同期在我院剖宫产分娩的30例产妇作为对照组。荧光定量PCR检测胎盘组织中miR-205、髓细胞白血病因子-1(myeloid cell leukemia-1,MCL-1)的表达水平。采用Western印迹法检测MCL-1蛋白表达。统计学方法采用独立样本t检验、Spearman线性相关分析法。结果子痫前期组与对照组孕妇的年龄和分娩孕周比较,差异无统计学意义(P>0.05);两组的收缩压、舒张压和24 h尿蛋白比较,差异有统计学意义(P<0.01)。子痫前期组孕妇胎盘组织中miR-205的2-△△Ct值高于对照组(0.226±0.043与0.117±0.031,P=0.000),MCL-1低于对照组(0.162±0.033与0.273±0.048,P=0.000)。Spearman相关分析显示,子痫前期组孕妇胎盘组织中miR-205表达与MCL-1表达呈负相关(r=-0.326,P<0.05)。子痫前期组孕妇胎盘组织中MCL-1蛋白表达水平低于对照组(0.164±0.035与0.436±0.092,P<0.01)。结论子痫前期患者胎盘组织中高表达的miR-205抑制MCL-1基因表达,可能参与子痫前期的致病过程。
Objective To detect the mRNA-205 expression in placenta of pregnant woman with preeclampsia and explore its effect in the pathogenesis of preeclampsia. Methods From October 2017 to July 2018, 30 patients of preeclampsia were enrolled in Dongguan People's Hospital as well as 30 cases of normal delivery were selected as control group. Real-time PCR was used to detect the expression of miR-205 in placenta and myeloid cell leukemia-1(MCL-1) mRNA. MCL-1 protein expression was detected by Western blot analysis. The statistical methods were carried out by independent sample t test and Spearman linear correlation analysis. Results There was no statistical significance in age and gestational week between preeclampsia group and control group(P>0.05). There was statistical significance in systolic blood pressure, diastolic blood pressure and 24-hour urinary protein between two groups(P<0.01).The 2--△△ Ctt values of miR-205 in placenta of preeclampsia group was higher than that in control group[(0.226±0.043) vs(0.117±0.031), P=0.000]. MCL-1 in preeclampsia group was lower than that in control group[(0.162±0.033) vs(0.273±0.048), P=0.000]. Spearman linear correlation analysis showed the expression of mi R-205 in placenta of pregnant women for preeclampsia group was negatively correlated with MCL-1(r=-0.326, P<0.05). The protein expression level of MCL-1 in placenta of pregnant women preeclampsia group was lower than that in control group[(0.164±0.035) vs(0.436±0.092),P<0.01]. Conclusion High expression of miR-205 in placenta of pregnant women for preeclampsia may inhibit the expression of MCL-1 to participate the pathogenesis of preeclampsia possibly.
Objective To detect the mRNA-205 expression in placenta of pregnant woman with preeclampsia and explore its effect in the pathogenesis of preeclampsia. Methods From October 2017 to July 2018, 30 patients of preeclampsia were enrolled in Dongguan People's Hospital as well as 30 cases of normal delivery were selected as control group. Real-time PCR was used to detect the expression of miR-205 in placenta and myeloid cell leukemia-1(MCL-1) mRNA. MCL-1 protein expression was detected by Western blot analysis. The statistical methods were carried out by independent sample t test and Spearman linear correlation analysis. Results There was no statistical significance in age and gestational week between preeclampsia group and control group(P>0.05). There was statistical significance in systolic blood pressure, diastolic blood pressure and 24-hour urinary protein between two groups(P<0.01).The 2--△△ Ctt values of miR-205 in placenta of preeclampsia group was higher than that in control group[(0.226±0.043) vs(0.117±0.031), P=0.000]. MCL-1 in preeclampsia group was lower than that in control group[(0.162±0.033) vs(0.273±0.048), P=0.000]. Spearman linear correlation analysis showed the expression of mi R-205 in placenta of pregnant women for preeclampsia group was negatively correlated with MCL-1(r=-0.326, P<0.05). The protein expression level of MCL-1 in placenta of pregnant women preeclampsia group was lower than that in control group[(0.164±0.035) vs(0.436±0.092),P<0.01]. Conclusion High expression of miR-205 in placenta of pregnant women for preeclampsia may inhibit the expression of MCL-1 to participate the pathogenesis of preeclampsia possibly.
引文
[1]Timofeeva AV,Gusar VA,Kan NE,et al.Identification of potential early biomarkers of preeclampsia[J].Placenta,2018,61(1):61-71.
[2]姚申珅,杜鹃.miRNA在子痫前期发病中的作用机制研究进展[J].山东医药,2018,58(3):103-105.
[3]Yang S,Li H,Ge Q,et al.Deregulated microRNA species in the plasma and placenta of patients with preeclampsia[J].Mol Med Rep,2015,12(1):527-534.
[4]中华医学会妇产科学分会妊娠期高血压疾病学组.妊娠期高血压疾病诊治指南(2015)[J].中华妇产科杂志,2015,50(10):721-728.
[5]柯华,李占魁.母亲妊娠期高血压疾病对新生儿预后的影响[J].发育医学电子杂志,2018,6(2):74-76.
[6]安刚,杨芳,余艳红.miRNA在子痫前期发病与诊断中的意义[J].现代妇产科进展,2016,25(3):229-231.
[7]王蒙蒙,刘卓琦,王晟,等.miRNA-205调控靶基因的研究进展与趋势[J].基础医学与临床,2015,35(1):112-116.
[8]郭俊,杨华强.Mcl-1分子研究进展[J].现代生物医学进展,2012,12(2):393-397.
[9]杨莹,苟文丽,谷寅,等.miR-26a-5p调控MCL-1在孕产妇子痫前期中表达的临床意义[J].中国病理生理杂志,2015,31(4):713-718.
[10]许燕滨,王晨虹.子痫前期中miR-17-5p表达及其对MCL-1的影响[J].现代妇产科进展,2018,27(3):161-164.
[11]阎雯,蔡玉香.下调miR-205抑制皮质神经元的缺血性损伤[J].神经解剖学杂志,2018,34(1):60-66.
[12]Soleymanlou N,Jurisicova A,Wu Y,et al.Hypoxic switch in mitochondrial myeloid cell leukemia factor-1/Mtd apoptotic rheostat contributes to human trophoblast cell death in preeclampsia[J].Am J Pathol,2007,171(2):496-506.
[2]姚申珅,杜鹃.miRNA在子痫前期发病中的作用机制研究进展[J].山东医药,2018,58(3):103-105.
[3]Yang S,Li H,Ge Q,et al.Deregulated microRNA species in the plasma and placenta of patients with preeclampsia[J].Mol Med Rep,2015,12(1):527-534.
[4]中华医学会妇产科学分会妊娠期高血压疾病学组.妊娠期高血压疾病诊治指南(2015)[J].中华妇产科杂志,2015,50(10):721-728.
[5]柯华,李占魁.母亲妊娠期高血压疾病对新生儿预后的影响[J].发育医学电子杂志,2018,6(2):74-76.
[6]安刚,杨芳,余艳红.miRNA在子痫前期发病与诊断中的意义[J].现代妇产科进展,2016,25(3):229-231.
[7]王蒙蒙,刘卓琦,王晟,等.miRNA-205调控靶基因的研究进展与趋势[J].基础医学与临床,2015,35(1):112-116.
[8]郭俊,杨华强.Mcl-1分子研究进展[J].现代生物医学进展,2012,12(2):393-397.
[9]杨莹,苟文丽,谷寅,等.miR-26a-5p调控MCL-1在孕产妇子痫前期中表达的临床意义[J].中国病理生理杂志,2015,31(4):713-718.
[10]许燕滨,王晨虹.子痫前期中miR-17-5p表达及其对MCL-1的影响[J].现代妇产科进展,2018,27(3):161-164.
[11]阎雯,蔡玉香.下调miR-205抑制皮质神经元的缺血性损伤[J].神经解剖学杂志,2018,34(1):60-66.
[12]Soleymanlou N,Jurisicova A,Wu Y,et al.Hypoxic switch in mitochondrial myeloid cell leukemia factor-1/Mtd apoptotic rheostat contributes to human trophoblast cell death in preeclampsia[J].Am J Pathol,2007,171(2):496-506.