六经头痛片对离体大鼠主动脉收缩及三叉神经降钙素基因相关肽表达的影响
|
摘要
目的观察六经头痛片对大鼠离体血管平滑肌收缩活动及三叉神经降钙素基因相关肽(CGRP)表达的影响。方法制备大鼠离体主动脉血管环,采用麦氏浴槽法观察六经头痛片对血管平滑肌收缩活动的影响;培养三叉神经节组织,观察六经头痛片对三叉神经节CGRP表达的影响。结果六经头痛片可以减弱KCl及去甲肾上腺素(NE)引起的胸主动脉环收缩反应,对KCl收缩的半数抑制浓度(IC_(50))为0.97 mg/m L,对NE收缩的IC_(50)为0.55 mg/m L。而且,六经头痛片在1.4、0.14mg/m L时可明显减少离体培养24、48 h后三叉神经节CGRP阳性细胞的数量(P<0.05)。结论六经头痛片既可以对抗血管平滑肌的收缩,又可以降低三叉神经具有扩张血管作用的CGRP的量,说明六经头痛片具有稳定血管舒缩的作用。
Objective To investigate the effects of Liujing Toutong Tablets(LTT) on isolated vascular smooth muscle and the expression of calcitonin gene-related peptide(CGRP) in trigeminus. Methods Rats' thoracic aorta and trigeminus were isolated to investigate the exact effect by measuring the contractile response of vascular and the expression of CGRP in trigeminus. Results The LTT could reduce the quantity of positive CGRP cells expressed in trigeminus(P < 0.05) and weaken the contractile response of thoracic aorta induced by KCl and NE, and the IC_(50) is 0.97 mg/m L and 0.55 mg/m L, respectively. Conclusion LTT could inhibit contractile response of vascular smooth muscle, meantime, reduce the quantity of positive CGRP cells expressed in trigeminus. The results show that LTT can stabilize vascular systaltic property.
Objective To investigate the effects of Liujing Toutong Tablets(LTT) on isolated vascular smooth muscle and the expression of calcitonin gene-related peptide(CGRP) in trigeminus. Methods Rats' thoracic aorta and trigeminus were isolated to investigate the exact effect by measuring the contractile response of vascular and the expression of CGRP in trigeminus. Results The LTT could reduce the quantity of positive CGRP cells expressed in trigeminus(P < 0.05) and weaken the contractile response of thoracic aorta induced by KCl and NE, and the IC_(50) is 0.97 mg/m L and 0.55 mg/m L, respectively. Conclusion LTT could inhibit contractile response of vascular smooth muscle, meantime, reduce the quantity of positive CGRP cells expressed in trigeminus. The results show that LTT can stabilize vascular systaltic property.
引文
[1]张晓燕,龚苏晓,游飞祥,等.基于GC-MS的六经头痛片挥发性化学成分研究[J].中草药,2016,47(21):3787-3794.
[2]熊玉兰,荆宇,尚明英,等.细辛非挥发性提取物抗炎镇痛作用研究[J].中国中药杂志,2009,34(17):2252-2257.
[3]陈奕芝,方若鸣,魏刚,等.石菖蒲挥发油、β-细辛醚对高脂血症大鼠血管舒缩与抗血小板聚集的作用[J].中国中西医结合杂志,2004,24(S1):16-18.
[4]杨小花,胡晓.欧前胡素与异欧前胡素的药理学研究进展[J].南昌大学学报:医学版,2012,52(3):95-97.
[5]常影.茺蔚子化学成分及药理研究[J].吉林中医药,2008,28(3):207-208.
[6]潘思源,常英,魏路香.茺蔚子总碱和水苏碱收缩离体小鼠子宫的比较[J].中草药,1998,29(10):687-688.
[7]范礼理,曾贵云,周远鹏,等.葛根的药理研究II:葛根黄酮对犬冠脉循环、心脏血流动力学和心肌代谢的作用[J].中华医学杂志,1975,55(10):724-727.
[8]Ko W C,Liao C C,Shih G H,et al.Relaxant effects of butylidenephthalide in isolated dog blood vessels[J].Planta Med,2002,68(1):1004-1009.
[9]沈雅琴,陈光娟,马树德.藁本中性油的镇静、镇痛、解热、抗炎作用[J].中西医结合杂志,1987,7(12):738-740.
[10]Olsen T S.Migraine with and without aura:the same disease due to cerebral vasospasm of different intensity a hypothosis based on CBF studies during migraine[J].Headache,1990,30(5):269-272.
[11]王玉洁,付峻,蔺慕慧,等.偏头痛[J].国外医学:脑血管疾病分册,2004,12(1):19-30.
[12]Goadsby P J,Edvinsson L,Ekman R.Vasoactive peptide release in the extracerebral circulation of humans during megraine headache[J].Ann Neurol,1990,28(2):183-187.
[13]Ashina M,Bendtsen L,Jensen R,et al.Evidence for increased plasma levels of calcitonin gene-related peptide in migraine outside of attacks[J].Pain,2000,86(1/2):133-138.
[14]Durham P L,Russo A F.New insights into molecular actions of serotonergic antimaigraine drugs[J].Phamccol Therapeutics,2002,94(1/2):77-92.
[15]Brain S D,Poyner D R,Hill R G.CGRP receptors:A headache to study,but will antagonists pmve therapeutic in migraine?[J].Trends Phamacol Sci,2002,23(2):51-53.
[16]Wimalawansa S J.Calcitonin gene-related peptide and its receptors:Molecular gengetics,physiology,pathophysiology,and thereapeutic potentials[J].EndoerRev,1996,17(5):533-585.
[17]Maggi C A.The mammalian tachykinin recepters[J].General Pharmacol,1995,26(5):911-944.
[18]Maggi C A.Tachykinin receptors and airway parhophysiology[J].Eur Respir J,1993,6(5):735-742.
[19]方玲,慕容慎行,张志坚,等.偏头痛患者血浆P物质与神经激肽A的变化[J].卒中与神经疾病,1997(3):20-22.
[20]陈佳颖,龙跃,吴飞翔,等.还原型谷胱甘肽对阻塞性黄疸大鼠胸主动脉收缩与舒张功能的影响[J].中华麻醉学杂志,2014,34(7):863-868.
[21]张小郁,李文广,郑天珍,等.葡萄籽中原花青素对实验动物主动脉收缩和血小板聚集的影响[J].中国应用生理学杂志,2005,21(4):383-386.
[22]汪海,路新强,张雁芳,等.吡那地尔和硝苯地平对大鼠离体血管的作用[J].中国药理学与毒理学杂志,1997,11(3):229-230.
[23]吴士清,周明正,朱俭.EDRF对NE引起的大鼠主动脉缩血管效应的作用[J].中国药理学通报,1997,13(3):235-238.
[24]郭竹英,饶曼人.高血压左室肥厚大鼠血管内皮的去留对NE和Ach反应的研究[J].中国药理学通报,1996,12(2):178-181.
[25]朱莉.血管平滑肌细胞的钙通道[J].国外医学:心血管疾病分册,1995,22(6):323-326.
[26]吕宝璋,卢运,安明榜.受体学[M].合肥:安徽科学技术出版社,2000.
[2]熊玉兰,荆宇,尚明英,等.细辛非挥发性提取物抗炎镇痛作用研究[J].中国中药杂志,2009,34(17):2252-2257.
[3]陈奕芝,方若鸣,魏刚,等.石菖蒲挥发油、β-细辛醚对高脂血症大鼠血管舒缩与抗血小板聚集的作用[J].中国中西医结合杂志,2004,24(S1):16-18.
[4]杨小花,胡晓.欧前胡素与异欧前胡素的药理学研究进展[J].南昌大学学报:医学版,2012,52(3):95-97.
[5]常影.茺蔚子化学成分及药理研究[J].吉林中医药,2008,28(3):207-208.
[6]潘思源,常英,魏路香.茺蔚子总碱和水苏碱收缩离体小鼠子宫的比较[J].中草药,1998,29(10):687-688.
[7]范礼理,曾贵云,周远鹏,等.葛根的药理研究II:葛根黄酮对犬冠脉循环、心脏血流动力学和心肌代谢的作用[J].中华医学杂志,1975,55(10):724-727.
[8]Ko W C,Liao C C,Shih G H,et al.Relaxant effects of butylidenephthalide in isolated dog blood vessels[J].Planta Med,2002,68(1):1004-1009.
[9]沈雅琴,陈光娟,马树德.藁本中性油的镇静、镇痛、解热、抗炎作用[J].中西医结合杂志,1987,7(12):738-740.
[10]Olsen T S.Migraine with and without aura:the same disease due to cerebral vasospasm of different intensity a hypothosis based on CBF studies during migraine[J].Headache,1990,30(5):269-272.
[11]王玉洁,付峻,蔺慕慧,等.偏头痛[J].国外医学:脑血管疾病分册,2004,12(1):19-30.
[12]Goadsby P J,Edvinsson L,Ekman R.Vasoactive peptide release in the extracerebral circulation of humans during megraine headache[J].Ann Neurol,1990,28(2):183-187.
[13]Ashina M,Bendtsen L,Jensen R,et al.Evidence for increased plasma levels of calcitonin gene-related peptide in migraine outside of attacks[J].Pain,2000,86(1/2):133-138.
[14]Durham P L,Russo A F.New insights into molecular actions of serotonergic antimaigraine drugs[J].Phamccol Therapeutics,2002,94(1/2):77-92.
[15]Brain S D,Poyner D R,Hill R G.CGRP receptors:A headache to study,but will antagonists pmve therapeutic in migraine?[J].Trends Phamacol Sci,2002,23(2):51-53.
[16]Wimalawansa S J.Calcitonin gene-related peptide and its receptors:Molecular gengetics,physiology,pathophysiology,and thereapeutic potentials[J].EndoerRev,1996,17(5):533-585.
[17]Maggi C A.The mammalian tachykinin recepters[J].General Pharmacol,1995,26(5):911-944.
[18]Maggi C A.Tachykinin receptors and airway parhophysiology[J].Eur Respir J,1993,6(5):735-742.
[19]方玲,慕容慎行,张志坚,等.偏头痛患者血浆P物质与神经激肽A的变化[J].卒中与神经疾病,1997(3):20-22.
[20]陈佳颖,龙跃,吴飞翔,等.还原型谷胱甘肽对阻塞性黄疸大鼠胸主动脉收缩与舒张功能的影响[J].中华麻醉学杂志,2014,34(7):863-868.
[21]张小郁,李文广,郑天珍,等.葡萄籽中原花青素对实验动物主动脉收缩和血小板聚集的影响[J].中国应用生理学杂志,2005,21(4):383-386.
[22]汪海,路新强,张雁芳,等.吡那地尔和硝苯地平对大鼠离体血管的作用[J].中国药理学与毒理学杂志,1997,11(3):229-230.
[23]吴士清,周明正,朱俭.EDRF对NE引起的大鼠主动脉缩血管效应的作用[J].中国药理学通报,1997,13(3):235-238.
[24]郭竹英,饶曼人.高血压左室肥厚大鼠血管内皮的去留对NE和Ach反应的研究[J].中国药理学通报,1996,12(2):178-181.
[25]朱莉.血管平滑肌细胞的钙通道[J].国外医学:心血管疾病分册,1995,22(6):323-326.
[26]吕宝璋,卢运,安明榜.受体学[M].合肥:安徽科学技术出版社,2000.