龙胆草有效成分与表型性状的相关性分析
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摘要
龙胆草(Gentiana scabra Bunge.)品种选育通常根据其生长发育状况并以高产作为目标实施群体选育,而品质育种没有可见的参考依据。能否通过表型性状确定品质优劣从而进行单株选育或特征类型群体选育是品质育种希望解决的问题。对采自2个地区100余份龙胆草进行表型性状分类并分级赋值,分别测定了样本中龙胆苦苷、獐牙菜苷、獐牙菜苦苷、熊果酸、齐墩果酸5种有效成分的含量,并分析了表型性状与有效成分之间的相关性。初步研究表明,龙胆草茎色、叶色等性状与龙胆苦苷等有效成分含量之间存在一定的相关性,可为以后的龙胆草株选或特征类型筛选提供参考。
With high yield as the goal,breeding of Gentiana scabra Bunge. is usually based on its growth and development status by group selection,but there is no reference for quality breeding.Whether individual plant breeding or characteristic type group breeding can be determined through phenotypic traits is a problem we want to solve with quality breeding. More than 100 plants collected from two areas were classified and grading assignment was conducted according to their phenotypic traits,after that,content of 5 effective constituents such as gentiopicroside,swertiamain,sweroside,oleanolic acid,ursolic acid was determined and correlation between phenotypic traits and effective constituents was analyzed. The preliminary studies show that there was some correlation between the main traits such as leaf color and the effective constituents such as gentiopicroside. And it can be used as valuable references for individual plant selection or characteristic type group selection of G.scabra Bunge.
With high yield as the goal,breeding of Gentiana scabra Bunge. is usually based on its growth and development status by group selection,but there is no reference for quality breeding.Whether individual plant breeding or characteristic type group breeding can be determined through phenotypic traits is a problem we want to solve with quality breeding. More than 100 plants collected from two areas were classified and grading assignment was conducted according to their phenotypic traits,after that,content of 5 effective constituents such as gentiopicroside,swertiamain,sweroside,oleanolic acid,ursolic acid was determined and correlation between phenotypic traits and effective constituents was analyzed. The preliminary studies show that there was some correlation between the main traits such as leaf color and the effective constituents such as gentiopicroside. And it can be used as valuable references for individual plant selection or characteristic type group selection of G.scabra Bunge.
引文
[1]李艳秋,赵德化,潘伯荣,等.龙胆苦甙抗鼠肝损伤的作用[J].第四军医大学学报,2001,22(18):1645-1649.
[2]佟丽,陈育尧,刘欢欢,等.龙胆粉针剂对实验性肝损伤的作用[J].第一军医大学学报,2001,21(12):906-907.
[3]陈雷,王海波,孙晓丽,等.龙胆苦苷镇痛抗炎药理作用研究[J].天然产物研究与开发,2008,20(5):903-906.
[4]胡若琪,饶娴宜.獐牙菜甙对D-氨基半乳糖性肝损伤大鼠脾脏体积的影响[J].云南中医学院学报,1989,12(2):8-9.
[5]Jaishree V,Badamis S.Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against D-galactosmine induced acute liver damage in rats[J].Journal Ethnopharmacology,2010,130(1):103-106.
[6]刘佩.解痉止痛新药——獐牙菜苦甙通过鉴定[J].药学通报,1984,19(7):63.
[7]Hitesh V,Mandapati R,Vasudevan S,et al.Swertiamarin:A lead from Enicostemma littorale Blume.for anti-hyperlipidaemic effect[J].European Journal of Pharmacology,2009,617:108-112.
[8]Li J,Guo W J,Yang Q Y.Effects of ursolic acid and oleanolic acid on human colon carcinoma cell line HCT15[J].World Journal Gastroenterol,2002,8(3):493-495.
[9]蔺美玲,陈红梅,张小郁,等.齐墩果酸抑制脂肪饮食肥胖大鼠体重的实验研究[J].成都医学院学报,2010,5(1):16-20.
[10]段宇,王冬梅,潘英妮,等.GAP基地龙胆中多糖和龙胆苦苷积累动态与土壤因子的关系[J].沈阳药科大学学报,2014,31(12):998-1001.
[11]宋九华,孟杰,曾宇,等.粗茎秦艽根茎品质与栽培土壤化学因子的相关性分析[J].植物资源与环境学报,2014,23(4):75-82.
[12]沈涛,杨美权,赵振玲,等.滇龙胆中萜类物质积累的动态变化[J].植物学报,2011,46(6):652-657.
[13]杨雁,邵爱娟,金航,等.云贵高原滇龙胆不同居群形态特征变异研究[J].中草药,2012,43(8):1604-1610.
[14]杨维泽,金航,杨美权,等.云南滇龙胆居群表型多样性及其与环境关系研究[J].西北植物学报,2011,31(7):1326-1334.
[15]周文平,赵洪峰,王亚飞,等.秦艽种质资源主要生物学特性与龙胆苦苷含量的相关分析[J].中药材,2015,38(5):933-936.
[2]佟丽,陈育尧,刘欢欢,等.龙胆粉针剂对实验性肝损伤的作用[J].第一军医大学学报,2001,21(12):906-907.
[3]陈雷,王海波,孙晓丽,等.龙胆苦苷镇痛抗炎药理作用研究[J].天然产物研究与开发,2008,20(5):903-906.
[4]胡若琪,饶娴宜.獐牙菜甙对D-氨基半乳糖性肝损伤大鼠脾脏体积的影响[J].云南中医学院学报,1989,12(2):8-9.
[5]Jaishree V,Badamis S.Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against D-galactosmine induced acute liver damage in rats[J].Journal Ethnopharmacology,2010,130(1):103-106.
[6]刘佩.解痉止痛新药——獐牙菜苦甙通过鉴定[J].药学通报,1984,19(7):63.
[7]Hitesh V,Mandapati R,Vasudevan S,et al.Swertiamarin:A lead from Enicostemma littorale Blume.for anti-hyperlipidaemic effect[J].European Journal of Pharmacology,2009,617:108-112.
[8]Li J,Guo W J,Yang Q Y.Effects of ursolic acid and oleanolic acid on human colon carcinoma cell line HCT15[J].World Journal Gastroenterol,2002,8(3):493-495.
[9]蔺美玲,陈红梅,张小郁,等.齐墩果酸抑制脂肪饮食肥胖大鼠体重的实验研究[J].成都医学院学报,2010,5(1):16-20.
[10]段宇,王冬梅,潘英妮,等.GAP基地龙胆中多糖和龙胆苦苷积累动态与土壤因子的关系[J].沈阳药科大学学报,2014,31(12):998-1001.
[11]宋九华,孟杰,曾宇,等.粗茎秦艽根茎品质与栽培土壤化学因子的相关性分析[J].植物资源与环境学报,2014,23(4):75-82.
[12]沈涛,杨美权,赵振玲,等.滇龙胆中萜类物质积累的动态变化[J].植物学报,2011,46(6):652-657.
[13]杨雁,邵爱娟,金航,等.云贵高原滇龙胆不同居群形态特征变异研究[J].中草药,2012,43(8):1604-1610.
[14]杨维泽,金航,杨美权,等.云南滇龙胆居群表型多样性及其与环境关系研究[J].西北植物学报,2011,31(7):1326-1334.
[15]周文平,赵洪峰,王亚飞,等.秦艽种质资源主要生物学特性与龙胆苦苷含量的相关分析[J].中药材,2015,38(5):933-936.