杨梅叶原飞燕草素对胰脂肪酶和磷脂酶A1的影响
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摘要
比较了奥利司他(Orlistat)以及不同加工程度的杨梅叶原飞燕草素提取物(杨梅叶粉末、杨梅叶含糖提取物和杨梅叶去糖提取物)对胰脂肪酶和磷脂酶A1在体外以及SD大鼠小肠内的活性抑制能力。结果表明:Orlistat对体外和大鼠小肠内的胰脂肪酶的活性抑制率均为最高,分别为99.0%和96.5%,排在第二的去糖提取物分别为90.9%和91.4%;Orlistat对体外和大鼠小肠内的磷脂酶A1的活性抑制率也均为最高,分别为97.1%和97.2%,排在第二的去糖提取物分别为96.7%和87.7%。检测了去糖提取物的半抑制浓度,对于胰脂肪酶和磷脂酶A1分别为0.01 mg/m L和0.06 mg/m L,分析其对胰脂肪酶的抑制类别为竞争性抑制+反竞争性抑制,对磷脂酶A1的抑制类别为竞争性抑制,杨梅叶原飞草素是一种潜在的高效、可控的新型减肥产品。
The effect of prodelphinidin extracts from Chinese bayberry leaves at different processing stages(leaf powder, non-desugarized prodelphinidin extract, and desugarized prodelphinidin extract) on pancreatic lipase and phospholipase A1 was evaluated in vitro and in the small intestine of SD rats in comparison with Orlistat. The results indicated that Orlistat had the highest inhibition percentage against pancreatic lipase in vitro and in the small intestine of SD rats, which were 99.0%and 96.5%, respectively, followed by the purified prodelphinidins(90.9% and 91.4%, respectively). Also, Orlistat had the highest inhibition percentage against phospholipase A1 in vitro and in the small intestine of SD rats, which were 97.1%and 97.2%, respectively, followed by the purified prodelphinidins(96.7% and 87.7%, respectively). The half maximal inhibitory concentration of the purified prodelphinidins against pancreatic lipase and phospholipase A1 were 0.01 and 0.06 mg/m L, respectively. The type of inhibition was competitive + uncompetitive for pancreatic lipase and was competitive for phospholipase A1. The purified prodelphinidins from Chinese bayberry leaves are potentially an efficient and controllable anti-obesity agent.
The effect of prodelphinidin extracts from Chinese bayberry leaves at different processing stages(leaf powder, non-desugarized prodelphinidin extract, and desugarized prodelphinidin extract) on pancreatic lipase and phospholipase A1 was evaluated in vitro and in the small intestine of SD rats in comparison with Orlistat. The results indicated that Orlistat had the highest inhibition percentage against pancreatic lipase in vitro and in the small intestine of SD rats, which were 99.0%and 96.5%, respectively, followed by the purified prodelphinidins(90.9% and 91.4%, respectively). Also, Orlistat had the highest inhibition percentage against phospholipase A1 in vitro and in the small intestine of SD rats, which were 97.1%and 97.2%, respectively, followed by the purified prodelphinidins(96.7% and 87.7%, respectively). The half maximal inhibitory concentration of the purified prodelphinidins against pancreatic lipase and phospholipase A1 were 0.01 and 0.06 mg/m L, respectively. The type of inhibition was competitive + uncompetitive for pancreatic lipase and was competitive for phospholipase A1. The purified prodelphinidins from Chinese bayberry leaves are potentially an efficient and controllable anti-obesity agent.
引文
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[22]黄巧珍,赵东海,裘观荣,等.杨梅叶总黄酮类化合物抗抑郁活性研究[J].时珍国医国药,2013,24(1):49-50.DOI:10.3969/j.issn.1008-0805.2013.01.022.
[23]UCHIDA S,HIRAI K,HATANAKA J,et al.Antinociceptive effects of St.John’s wort,Harpagophytum procumbens extract and grape seed proanthocyanidins extract in mice[J].Biological&Pharmaceutical Bulletin,2008,31(2):240-245.DOI:10.1248/bpb.31.240.
[24]YANG Haihua,YE Xingqian,LIU Donghong,et al.Characterization of unusual proanthocyanidins in leaves of bayberry(Myrica rubra Sieb.et Zucc.)[J].Journal of Agricultural and Food Chemistry,2011,59(5):1622-1629.DOI:10.1021/jf103918v.
[25]LAI Y F,LAWRENCE J P.Prodelphinidin polymers:definition of structural units[J].Journal of the Chemical Society,Perkin Transactions 1,1978(10):1186-1190.DOI:10.1039/P19780001186.
[26]周晓舟,曹玉敏,孙玉敬,等.大孔树脂对杨梅叶原花色素的纯化去糖研究[J].离子交换与吸附,2016,32(2):154-163.DOI:10.16026/j.cnki.iea.2016020154.
[27]KOGA K,HISAMURA M,KANETAKA T,et al.Proanthocyanidin oligomers isolated from Salacia reticulata leaves potently inhibit pancreatic lipase activity[J].Journal of Food Science,2013,78(1):105-111.DOI:10.1111/1750-3841.12001.
[28]郭诤,张根旺.脂肪酶的结构特征和化学修饰[J].中国油脂,2003,28(7):5-7.DOI:10.3321/j.issn:1003-7969.2003.07.001.
[29]李脉,杨继国,杨博.磷脂酶A1酶活测定方法的研究[J].现代食品科技,2007,23(8):80-82.DOI:10.3969/j.issn.1673-9078.2007.08.025.
[30]PARK S H,HUH T L,KIM S Y,et al.Antiobesity effect of Gynostemma pentaphyllum extract(actiponin):a randomized,doubleblind,placebo-controlled trial[J].Obesity,2014,22(1):63-71.DOI:10.1002/oby.20539.
[2]石碧,杜晓.植物原花色素研究利用进展与发展趋势[J].四川大学学报(工程科学版),2006,38(5):16-24.DOI:10.3969/j.issn.1009-3087.2006.05.003.
[3]陆茵,孙志广,赵万洲,等.原花青素抗促癌物诱发H2O2释放及脂质过氧化[J].中国药理学通报,2001,17(5):562-565.DOI:10.3321/j.issn:1001-1978.2001.05.024.
[4]屈景年,曾文良,陈辉,等.葡萄籽原花色素的提取、改性及抗氧化性研究[J].衡阳师范学院学报,2010,31(3):48-51.DOI:10.3969/j.issn.1673-0313.2010.03.013.
[5]钟瑞敏,张振明,王羽梅,等.杨梅树叶、皮、根部精油成分及其抗氧化活性物质[J].林产化学与工业,2006,26(1):1-5.DOI:10.3321/j.issn:0253-2417.2006.01.001.
[6]ADEDAPO A A,JIMOH F O,KODURU S,et al.Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea[J].BMC Complementary and Alternative Medicine,2008,8:53.DOI:10.1186/1472-6882-8-53.
[7]GUNARATNE A,WU K,LI D,et al.Antioxidant activity and nutritional quality of traditional red-grained rice varieties containing proanthocyanidins[J].Food Chemistry,2013,138(2/3):1153-1161.DOI:10.1016/j.foodchem.2012.11.129.
[8]LEE Y A,KIM Y J,CHO E J,et al.Ameliorative effects of proanthocyanidin on oxidative stress and inflammation in streptozotocin-induced diabetic rats[J].Journal of Agricultural and Food Chemistry,2007,55(23):9395-9400.DOI:10.1021/jf071523u.
[9]张冰若,劳业兴,苏薇薇.原花青素的研究现状及开发前景[J].中药材,2003,26(12):905-908.DOI:10.3321/j.issn:1001-4454.2003.12.026.
[10]杨玉红,张慧霞.葡萄原花青素的生物学活性的简介[J].生物学通报,2008,43(9):16-18.DOI:10.3969/j.issn.0006-3193.2008.09.007.
[11]周素娟.葡萄籽提取物原花青素的研究概况及其在我国保健食品中的应用[J].中国食品卫生杂志,2007,19(3):284-286.DOI:10.3969/j.issn.1004-8456.2007.03.029.
[12]张华,曾桥.原花青素功能及应用进展[J].安徽农业科学,2011,39(9):5349-5350.DOI:10.3969/j.issn.0517-6611.2011.09.115.
[13]崔介君,孙培龙,马新.原花青素的研究进展[J].食品科技,2003(2):92-95.DOI:10.3969/j.issn.1005-9989.2003.02.034.
[14]孙传范.原花青素的研究进展[J].食品与机械,2010,26(4):146-148.DOI:10.3969/j.issn.1003-5788.2010.04.042.
[15]凌智群,谢笔钧,江涛,等.莲房原花青素对大鼠实验性心肌缺血的保护作用[J].中国药理学通报,2001,17(6):687-690.DOI:10.3321/j.issn:1001-1978.2001.06.024.
[16]张小郁,李文广,高明堂,等.葡萄籽中原花青素对心肌细胞的保护作用[J].中药药理与临床,2001,17(6):14-16.DOI:10.3969/j.issn.1001-859X.2001.06.008.
[17]TOMARU M,TAKANO H,OSAKABE N,et al.Dietary supplementation with cacao liquor proanthocyanidins prevents elevation of blood glucose levels in diabetic obese mice[J].Nutrition,2007,23(4):351-355.DOI:10.1016/j.nut.2007.01.007.
[18]赵万洲,陆茵,闫新琦,等.葡萄籽原花青素抗促癌作用的实验研究[J].中草药,2000,31(12):917-920.DOI:10.3321/j.issn:0253-2670.2000.12.023.
[19]DAYCEM K,RABBIAA M S,JALLOUL B,et al.Composition and anti-oxidant,anti-cancer and anti-inflammatory activities of Artemisia herba-alba,Ruta chalpensis L.and Peganum harmala L.[J].Food and Chemical Toxicology,2013,55:202-208.DOI:10.1016/j.fct.2013.01.004.
[20]WU C H,YANG M Y,CHAN K C,et al.Improvement in high-fat diet-induced obesity and body fat accumulation by a nelumbo nucifera leaf flavonoid-rich extract in mice[J].Journal of Agricultural and Food Chemistry,2010,58(11):7075-7081.DOI:10.1021/jf101415v.
[21]KANAMOTO Y,YAMASHITA Y,NANBA F,et al.A black soybean seed coat extract prevents obesity and glucose intolerance by upregulating uncoupling proteins and down-regulating inflammatory cytokines in high-fat diet-fed mice[J].Journal of Agricultural and Food Chemistry,2011,59(16):8985-8993.DOI:10.1021/jf201471p.
[22]黄巧珍,赵东海,裘观荣,等.杨梅叶总黄酮类化合物抗抑郁活性研究[J].时珍国医国药,2013,24(1):49-50.DOI:10.3969/j.issn.1008-0805.2013.01.022.
[23]UCHIDA S,HIRAI K,HATANAKA J,et al.Antinociceptive effects of St.John’s wort,Harpagophytum procumbens extract and grape seed proanthocyanidins extract in mice[J].Biological&Pharmaceutical Bulletin,2008,31(2):240-245.DOI:10.1248/bpb.31.240.
[24]YANG Haihua,YE Xingqian,LIU Donghong,et al.Characterization of unusual proanthocyanidins in leaves of bayberry(Myrica rubra Sieb.et Zucc.)[J].Journal of Agricultural and Food Chemistry,2011,59(5):1622-1629.DOI:10.1021/jf103918v.
[25]LAI Y F,LAWRENCE J P.Prodelphinidin polymers:definition of structural units[J].Journal of the Chemical Society,Perkin Transactions 1,1978(10):1186-1190.DOI:10.1039/P19780001186.
[26]周晓舟,曹玉敏,孙玉敬,等.大孔树脂对杨梅叶原花色素的纯化去糖研究[J].离子交换与吸附,2016,32(2):154-163.DOI:10.16026/j.cnki.iea.2016020154.
[27]KOGA K,HISAMURA M,KANETAKA T,et al.Proanthocyanidin oligomers isolated from Salacia reticulata leaves potently inhibit pancreatic lipase activity[J].Journal of Food Science,2013,78(1):105-111.DOI:10.1111/1750-3841.12001.
[28]郭诤,张根旺.脂肪酶的结构特征和化学修饰[J].中国油脂,2003,28(7):5-7.DOI:10.3321/j.issn:1003-7969.2003.07.001.
[29]李脉,杨继国,杨博.磷脂酶A1酶活测定方法的研究[J].现代食品科技,2007,23(8):80-82.DOI:10.3969/j.issn.1673-9078.2007.08.025.
[30]PARK S H,HUH T L,KIM S Y,et al.Antiobesity effect of Gynostemma pentaphyllum extract(actiponin):a randomized,doubleblind,placebo-controlled trial[J].Obesity,2014,22(1):63-71.DOI:10.1002/oby.20539.