2016年河南省分离自患者粪便的志贺菌分型及耐药分析
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摘要
目的分析2016年河南省腹泻患者志贺菌感染的血清型分布及其耐药性。方法对2016年从河南省15家哨点医院中符合病例定义的2 896名患者的粪便中分离的50株志贺菌进行血清学分型,并采用肉汤稀释法进行药敏试验。结果 50株志贺菌以福氏志贺菌和宋内志贺菌2种血清型为主,其中福氏志贺菌2a型为引起食源性腹泻的主要病原菌菌型,占比52.0%(26/50),其次是宋内志贺菌II相,占比26.0%(13/50);耐药率最高的抗生素是萘啶酸(100.0%,50/50)和四环素(100.0%,50/50),其他依次为氨苄西林(98.0%,49/50)和甲氧苄氨嘧啶/磺胺甲噁唑(90.0%,45/50)。结论志贺菌多重耐药现象严重。
Objective Analyze the serotype distribution and drug resistance of Shigella from patients in Henan Province in 2016. Methods Fifty Shigella strains which were isolated from 2 896 diarrhea patients by 15 sentinel hospitals in Henan Province were tested for serotype and drug susceptibility with broth dilution method, and etiological food information were collected and analyzed statistically. Results Shigella flexneri and Shigella soonei were dominant serotype, among which Shigella flexneri 2 a subtype was the No.1 pathogens, accounted for 52.0%(26/50), followed by Shigella soonei II phase accounted for 26.0%(13/50). For drug susceptibility test for 10 kinds of antibiotics, the antibiotics with high resistance for Shigella included nalidixic acid(100.0%,50/50), tetracycline(100.0%,50/50), ampicillin(98.0%,49/50) and sulfonamide(90.0%,45/50). Conclusion The multidrug resistance was serious.
Objective Analyze the serotype distribution and drug resistance of Shigella from patients in Henan Province in 2016. Methods Fifty Shigella strains which were isolated from 2 896 diarrhea patients by 15 sentinel hospitals in Henan Province were tested for serotype and drug susceptibility with broth dilution method, and etiological food information were collected and analyzed statistically. Results Shigella flexneri and Shigella soonei were dominant serotype, among which Shigella flexneri 2 a subtype was the No.1 pathogens, accounted for 52.0%(26/50), followed by Shigella soonei II phase accounted for 26.0%(13/50). For drug susceptibility test for 10 kinds of antibiotics, the antibiotics with high resistance for Shigella included nalidixic acid(100.0%,50/50), tetracycline(100.0%,50/50), ampicillin(98.0%,49/50) and sulfonamide(90.0%,45/50). Conclusion The multidrug resistance was serious.
引文
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[14] BALLEST-DELPIERRE C, SOL M, DOMNECH O,et al. Molecular study of quinolone resistance mechanisms and clonal relationship of Salmonella enterica clinical isolates[J]. Int J Antimicrob Agents, 2014,43(2):121-125.
[2] 贾肇一,马洪生,苏明,等.一起宋内志贺菌暴发的病原学鉴定及其药敏和毒力基因检测结果分析[J].中国卫生检验杂志,2013,23(15):3062-3064.
[3] 国家食品安全风险评估中心.2015年食源性疾病监测工作手册[Z].2015:6.
[4] 中华人民共和国卫生部. 食品安全国家标准 食品微生物学检验 志贺氏菌检验:GB 4789.5—2012[S].北京:中国标准出版社,2012.
[5] Clinical and Laboratory Standards Institute.M100-S25 performance standards for antimicrobial susceptibility testing: twenty-fifth informational supplement[S]. Wayne, PA: CLSI, 2015.
[6] 宋信兵.2009—2010年睢县致泻性病原菌监测结果分析[J].河南预防医学杂志,2011,22(5):370-371.
[7] CHOMPOOK P, TODD J, WHEELER J G, et al. Risk factors for shigellosis in Thailand[J]. Int J Infect Dis,2006,10(6):425-433.
[8] 沈丽珍,徐斐,余玲玲,等.浙江省温州地区志贺菌流行菌株的血清分型及耐药性分析[J].疾病监测,2009,24(5):334-336.
[9] 张广业,于德山,汪鹏,等.甘肃省2005—2011年志贺菌菌型变迁及耐药性监测分析[J].中国卫生检验杂志,2012,22(9):2093-2095.
[10] 李春,孙永,张钧,等.临床分离34株志贺氏菌菌群分布及耐药性分析[J].安徽预防医学杂志,2014,20(1):1-3.
[11] 徐彩萍,张颖华,陈秀华,等.129株志贺菌血清分型及耐药性分析[J].职业与健康,2013,29(6):702-704.
[12] 夏依旦·吾甫尔,木塔力甫·托呼提,张健,等.新疆痢疾监测地区志贺氏菌菌型分布及耐药性分析[J].疾病预防控制通报,2017,32(1):5-9.
[13] 毕宇涵,李鑫,董锐,等.46株福氏志贺氏菌耐药及PFGE分子分型分析[J]. 中国公共卫生管理,2016,32(1):85-87.
[14] BALLEST-DELPIERRE C, SOL M, DOMNECH O,et al. Molecular study of quinolone resistance mechanisms and clonal relationship of Salmonella enterica clinical isolates[J]. Int J Antimicrob Agents, 2014,43(2):121-125.