利用一株凝结芽孢杆菌发酵酸解玉米秸秆生产乳酸
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摘要
该研究拟考察凝结芽孢杆菌(Bacillus coagulans)利用玉米秸秆生物炼制乳酸的效果。以凝结芽孢杆菌CGMCC No.7635为菌种,利用2%硫酸预处理后的玉米秸秆为碳源、20 g/L酵母粉为氮源,添加20 FPU/g纤维素酶后开展糖化发酵生产乳酸实验。结果表明,发酵65 h后可获得乳酸含量为(38.38±1.03)g/L,其中L-乳酸光学纯度为(99.23±0.22)%。进一步使用补料发酵工艺,添加经预处理的玉米秸秆,可最终获得乳酸含量为(82.56±1.28)g/L。建立的玉米秸秆生物炼制乳酸工艺操作简单、产物浓度高,具有工业应用潜力。
To study the effect of lactic acid biorefinery from corn stalk by Bacillus coagulans, using B. coagulans CGMCC No.7635 as strain, corn stalk pretreated by sulfuric acid 2% as carbon source, and yeast extract 20 g/L as nitrogen source, the lactic acid was produced by simultaneous saccharification and fermentation tests after adding 20 FPU/g cellulose. The results showed that the lactic acid production was(38.38±1.03) g/L after fermentation for 65 h. Among them, the optical purity of L-lactic acid was(99.23±0.22)%. By fed-batch fermentation process with adding pretreated corn stalk, the lactic acid production was(82.56±1.28) g/L. The established biorefinery lactic acid process from corn stalk had simple operation, high product concentration and potential for industrial application.
To study the effect of lactic acid biorefinery from corn stalk by Bacillus coagulans, using B. coagulans CGMCC No.7635 as strain, corn stalk pretreated by sulfuric acid 2% as carbon source, and yeast extract 20 g/L as nitrogen source, the lactic acid was produced by simultaneous saccharification and fermentation tests after adding 20 FPU/g cellulose. The results showed that the lactic acid production was(38.38±1.03) g/L after fermentation for 65 h. Among them, the optical purity of L-lactic acid was(99.23±0.22)%. By fed-batch fermentation process with adding pretreated corn stalk, the lactic acid production was(82.56±1.28) g/L. The established biorefinery lactic acid process from corn stalk had simple operation, high product concentration and potential for industrial application.
引文
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[21]QIU Z Y,GAO Q Q,BAO J.Constructing xylose-assimilating pathways in Pediococcus acidilactici for high titer D-lactic acid fermentation from corn stover feedstock[J].Bioresource Technol,2017,245:1369-1376.
[2]薛海燕,李均敏.玉米秸秆同步糖化发酵生产乳酸的研究[J].中国酿造,2008,27(18):37-40.
[3]迟燕平.共固定化体系协同转化玉米秸秆生产乳酸研究[J].食品工业,2014,35(9):1-4.
[4]刘占英,马慧芳,段申,等.紫外诱变提高一株粪肠球菌产L-乳酸的能力[J].内蒙古农业大学学报,2013,34(6):5-8.
[5]李鑫,马瑞,赵晗璐,等.米根霉利用纯糖和不同预处理玉米秸秆酶解糖生产L-乳酸[J].生物加工过程,2010,8(6):1-5.
[6]王刚,武丽达,张明磊,等.玉米秸秆同步糖化生物炼制D-乳酸的工艺研究[J].吉林农业大学学报,2016,38(5):562-565.
[7]陈晓佩,张丽,顾夕梅,等.米根霉无载体固定化发酵产L-乳酸[J].林产化学与工业,2014,34(5):127-132.
[8]张玉明.凝结芽孢杆菌在乳酸生物炼制中的应用[J].中国酿造,2017,36(2):10-14.
[9]ZHANG Y M,CHEN X R,QI B K,et al.Improving lactic acid productivity from wheat straw hydrolysates by membrane integrated repeated batch fermentation under non-sterilized conditions[J].Bioresource Technol,2014,163:160-166.
[10]COTNANA F,CAVALAGLIO G,PISELLOO A L,et al.Sustainable ethanol production from common reed(Phragmites australis)through simultaneous saccharification and fermentation[J].Sustainability,2015,7:12149-12163.
[11]GORKE B,STULKE J.Catabolite repression in bacteria:many ways to make the most out of nutrients[J].Nat Rev Microbiol,2008,6(8):613-624.
[12]XIAO H,LI ZL,JIANG Y,et al.Metabolic engineering of D-xylose pathway in Clostridium beijerinckii to optimize solvent production from xylose mother liquid[J].Metab Eng,2012,14(5):569-578.
[13]ABDELRHMAN M,XIAO Y,TASHIRO Y.Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression[J].J Biosci Bioeng,2015,119(2):153-158.
[14]张勇,郭栋豪,王克勤,等.γ射线辐照预处理玉米秸秆发酵产乙醇的研究[J].中国酿造,2018,37(9):58-61.
[15]LI X,XIAO Y,FENG Y,et al.The spatial proximity effect of beta-glucosidase and cellulosomes on cellulose degradation[J].Enzyme Microb Tech,2018,115:52-61.
[16]UNREAN P.Optimized feeding schemes of simultaneous saccharification and fermentation process for high lactic acid titer from sugarcane bagasse[J].Ind Crop Prod,2018,111:660-666.
[17]ENRIQUE C C,CRISTINA G F,MERCEDES B,et al.Biotechnological advances in lactic acid production by lactic acid bacteria:Lignocellulose as novel substrate[J].Biofuels Bioprod Biorefining,2018,12(2):290-303.
[18]LAU M W,DALE B E.Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST)[J].P Natl Acad Sci USA,2009,106(5):1368-1373.
[19]OU M S,AWASTHI D,NIEVES I,et al.Sweet sorghum juice and bagasse as feedstocks for the production of optically pure lactic acid by native and engineered Bacillus coagulans strains[J].Bioenerg Res,2016,9(1):123-131.
[20]HU J L,LIN Y X,ZHANG Z T,et al.High-titer lactic acid production by Lactobacillus pentosus FL0421 from corn stover using fed-batch simultaneous saccharification and fermentation[J].Bioresource Technol,2016,214:74-80.
[21]QIU Z Y,GAO Q Q,BAO J.Constructing xylose-assimilating pathways in Pediococcus acidilactici for high titer D-lactic acid fermentation from corn stover feedstock[J].Bioresource Technol,2017,245:1369-1376.