脐疗对寒凝血瘀型痛经大鼠神阙穴区Toll样受体2表达的影响
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摘要
目的观察脐疗对寒凝血瘀型痛经大鼠神阙穴区Toll样受体2(Toll-like Receptors,TLR2)表达的影响,初步探索脐疗效应与神阙穴区的关系,阐释部分启动机制。方法将50只雌性大鼠随机分为正常对照组、模型组、药物贴脐组、隔药灸脐组、艾条灸脐组,每组10只。除正常对照组外,其余各组均构建寒凝血瘀痛经模型,给予相应的处理及治疗。通过免疫荧光法、蛋白质免疫印迹法、实时荧光定量PCR技术检测各组大鼠神阙穴区TLR2的表达。结果药物贴脐组、隔药灸脐组及艾条灸脐组TLR2蛋白表达量较模型组均升高,艾条灸脐组TLR2蛋白表达量高于隔药灸脐组、药物贴脐组(P<0.01);药物贴脐组、隔药灸脐组及艾条灸脐组TLR2mRNA表达较模型组均明显升高(P<0.01),艾条灸脐组高于隔药灸脐组和药物贴脐组(P<0.01)。结论脐疗通过上调寒凝血瘀型痛经大鼠神阙穴区TLR2蛋白及mRNA表达以激活腧穴功能,是诱导神阙信息启动的部分机制。
Objective To observe the effect of navel therapy on the expression of(Toll-like Receptors 2)TLR2 in Shenque of coagulated cold and blood stasis type dysmenorrhea model rats, to initially explore the relationship between the umbilical effect and Shenque, and explain part of the initiation mechanism. Methods Fifty female rats were randomly divided into five groups: normal control group, model group, drug sticking navel group,Herb-partitioned moxibustion on navel group, moxa moxibustion navel group, 10 in each group. In addition to normal control group, coagulated Cold and Blood Stasis dysmenorrhea model was constructed. We gave esch group the corresponding treatment. The expression of TLR2 in Shenque was detected by immunofluorescence, Western blotting and Quantitative Real-time PCR. Results The expression of TLR2 in the navel treatment groups were higher than that in model group. The expression of TLR2 in moxa moxibustion navel group was higher than that in drug sticking navel group and Herb-partitioned moxibustion on navel group(P<0.01);the expression of TLR2 mRNA in the navel treatment groups were higher than that in model group(P<0.01).The expression of TLR2 mRNA in moxa moxibustion navel group was higher than that in drug sticking navel group and Herb-partitioned moxibustion on navel group(P<0.01). Conclusion Navel therapy activates the acupoint function by up-regulating the expression of TLR2 and TLR2 mRNA in the Shenque of coagulated Cold and Blood Stasis type dysmenorrhoea rat, is part of the mechanism for inducing the initiation of Shenque information.
Objective To observe the effect of navel therapy on the expression of(Toll-like Receptors 2)TLR2 in Shenque of coagulated cold and blood stasis type dysmenorrhea model rats, to initially explore the relationship between the umbilical effect and Shenque, and explain part of the initiation mechanism. Methods Fifty female rats were randomly divided into five groups: normal control group, model group, drug sticking navel group,Herb-partitioned moxibustion on navel group, moxa moxibustion navel group, 10 in each group. In addition to normal control group, coagulated Cold and Blood Stasis dysmenorrhea model was constructed. We gave esch group the corresponding treatment. The expression of TLR2 in Shenque was detected by immunofluorescence, Western blotting and Quantitative Real-time PCR. Results The expression of TLR2 in the navel treatment groups were higher than that in model group. The expression of TLR2 in moxa moxibustion navel group was higher than that in drug sticking navel group and Herb-partitioned moxibustion on navel group(P<0.01);the expression of TLR2 mRNA in the navel treatment groups were higher than that in model group(P<0.01).The expression of TLR2 mRNA in moxa moxibustion navel group was higher than that in drug sticking navel group and Herb-partitioned moxibustion on navel group(P<0.01). Conclusion Navel therapy activates the acupoint function by up-regulating the expression of TLR2 and TLR2 mRNA in the Shenque of coagulated Cold and Blood Stasis type dysmenorrhoea rat, is part of the mechanism for inducing the initiation of Shenque information.
引文
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[14] 盖晓丽.隔药灸脐与艾条灸脐对原发性痛经患者症状及子宫动脉血流动力学的影响[D].山东中医药大学硕士学位论文,2013:8.
[15] 薛哲,刘存志,高树中,等.隔药灸治疗原发性痛经及对患者生殖内分泌的影响[J].中国针灸,2014,34(3):209.
[16] 许咏思,稽波,胡琅琳,等.寒凝血瘀证痛经大鼠模型制备和评价方法述评[J].中医药学报,2013,41(4):25.
[17] Stokes AJ,Shimoda LM,Koblan-Huberson M,et al.A TRPV2-PKA signaling module for transduction of physical stimuli in mast cells[J].J Exp Med ,2004,19,200(2):137.
[18] 秦稳,周国华.Toll样受体与肠道相关疾病的研究进展[J].医学综述,2010,16(19):2921.
[19] 金伯泉.固有免疫中模式识别受体及其信号转导—当代免疫学中最伟大的发现之一[J].细胞与分子免疫学杂志,2006,22(1):1.
[20] 杨海伟,魏继福,何韶衡.肥大细胞Toll样受体的研究进展[J].细胞与分子免疫学杂志,2008,24(9):933.
[21] 卢圣锋,尹海燕,唐勇,等.瞬时感受器电位及其与艾灸温热效应机制研究结合的思考[J].针刺研究,2012,37(2):151.
[2] 孙玉阳,纪宏宇,陈博,等.原发性痛经的发病机制及中医药治疗的研究进展[J].中国药师,2017,20(1):144.
[3] 郭瑞玲.当归四逆汤加减联合TDP治疗原发性痛经寒湿凝滞证临床观察[J].新中医,2018,50(3):131.
[4] Proctor M,Farquhar C.Diagnosis and management of dysmenorrhoea[J].Brit Med J,2006,332(7550):1134.
[5] Hong J,Mark Jones,Gita Mishra.The prevalence and risk factors of dysmenorrhea[J].Epidemiol Rev,2014,36:104.
[6] 王红琴.160名卫校女生痛经状况及影响因素分析[J].中国校医,2007,21(4):429.
[7] 孙艳明,王玲,李戈.1800名女大学生痛经影响因素调查分析[J].天津中医药,2009,26(5):367.
[8] 李芳,汪坤秀,陈光英.粤西高校女大学生原发性痛经相关因素的调查分析[J].临床护理杂志,2009,8(1):12.
[9] 陈文,俞海虹,刘世红,等.国内针灸治疗原发性痛经的系统评价[J].中华中医药学刊,2013,31(2):321.
[10] 杨兵文.脐部外敷配合针灸治疗寒凝血瘀型原发性痛经临床观察[J].新中医,2016,48(8):164.
[11] 韩双伊,王翠霞.针灸治疗痛经的临床研究进展[J].实用中医内科杂志,2012,26(18):91.
[12] 李姝婧,张晓宁,马凤君,等.脐疗对寒凝血瘀型痛经大鼠神阙穴区肥大细胞的影响[J].时珍国医国药,2017,28(9):2288.
[13] 杜冬青.隔药灸脐法治疗原发性痛经的经穴效应特异性研究[D].山东中医药大学博士学位论文,2013:93.
[14] 盖晓丽.隔药灸脐与艾条灸脐对原发性痛经患者症状及子宫动脉血流动力学的影响[D].山东中医药大学硕士学位论文,2013:8.
[15] 薛哲,刘存志,高树中,等.隔药灸治疗原发性痛经及对患者生殖内分泌的影响[J].中国针灸,2014,34(3):209.
[16] 许咏思,稽波,胡琅琳,等.寒凝血瘀证痛经大鼠模型制备和评价方法述评[J].中医药学报,2013,41(4):25.
[17] Stokes AJ,Shimoda LM,Koblan-Huberson M,et al.A TRPV2-PKA signaling module for transduction of physical stimuli in mast cells[J].J Exp Med ,2004,19,200(2):137.
[18] 秦稳,周国华.Toll样受体与肠道相关疾病的研究进展[J].医学综述,2010,16(19):2921.
[19] 金伯泉.固有免疫中模式识别受体及其信号转导—当代免疫学中最伟大的发现之一[J].细胞与分子免疫学杂志,2006,22(1):1.
[20] 杨海伟,魏继福,何韶衡.肥大细胞Toll样受体的研究进展[J].细胞与分子免疫学杂志,2008,24(9):933.
[21] 卢圣锋,尹海燕,唐勇,等.瞬时感受器电位及其与艾灸温热效应机制研究结合的思考[J].针刺研究,2012,37(2):151.