人参皂苷抑菌活性研究进展
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摘要
随着抗生素的广泛使用,临床耐药菌的数量和种类在不断增多,耐药机制也呈现多样性的趋势,其中由于细菌生物被膜而产生的细菌耐药成为当前研究的重点和难点。中药抑菌作用因其高效性和低毒副作用等特点已成为目前抑菌研究的热点。人参皂苷是人参的主要有效物质,具有多种药理作用。本文结合近年对人参皂苷抑菌、抑制细菌生物膜及相应机制、以及与其他药物协同增效抑菌的研究进行总结,综述了目前人参皂苷抑菌活性的研究进展。
The quantity and species of drug-resistant bacteria have increased with the widespread use of antibiotics,and the mechanisms of their resistance have tended to vary.Drug resistance caused by bacterial biofilm has become the focus and a sticking point of current research.At present,almost all strains have developed antibiotic resistance.Solutions to the problem of bacterial resistance need to be promptly found.Traditional Chinese medicine has become a focus of bacteriostatic research due to its higher efficiency and less toxic adverse effects.GinsengPanaxis the most famous traditional Chinese medicine.Ginsenoside is one of the main active substances in ginseng,and it has various pharmacological effects.This paper describes the antibacterial advantages of this traditional Chinese medicine and its mechanism of action.This paper also summarizes studies on the antibacterial activity of ginsenoside,its inhibition of bacterial biofilm and the mechanism of that inhibition,and the synergistic effect of ginsenoside on bacteriostatic activity.In addition,this paper examines the limitations of and prospects for use of ginsenoside in bacteriostatic research.
The quantity and species of drug-resistant bacteria have increased with the widespread use of antibiotics,and the mechanisms of their resistance have tended to vary.Drug resistance caused by bacterial biofilm has become the focus and a sticking point of current research.At present,almost all strains have developed antibiotic resistance.Solutions to the problem of bacterial resistance need to be promptly found.Traditional Chinese medicine has become a focus of bacteriostatic research due to its higher efficiency and less toxic adverse effects.GinsengPanaxis the most famous traditional Chinese medicine.Ginsenoside is one of the main active substances in ginseng,and it has various pharmacological effects.This paper describes the antibacterial advantages of this traditional Chinese medicine and its mechanism of action.This paper also summarizes studies on the antibacterial activity of ginsenoside,its inhibition of bacterial biofilm and the mechanism of that inhibition,and the synergistic effect of ginsenoside on bacteriostatic activity.In addition,this paper examines the limitations of and prospects for use of ginsenoside in bacteriostatic research.
引文
[1]黄玉兰.抗生素药物不良反应与合理应用的临床措施探讨[J].北方药学,2017,14(1):145-6.
[2]李睿明,雷朝霞.细菌耐药性对抗策略--中药延缓、逆转细菌耐药性,治疗耐药细菌感染的研究[J].医学与哲学(临床决策论坛版),2006,27(8):45-7.
[3]胡晋婷,马萍,陈瑶,等.中药及活性成分对细菌生物膜防治研究近况[J].亚太传统医药,2016,12(24):59-61.
[4]李亚娜,陶庆春.中药抑菌的研究现状及思考[J].国际检验医学杂志,2014,35(2):198-200.
[5]戚健君,王文林,李树德.人参皂干Rg1对NOX3致斯氏狸殖吸虫肝纤维化的影响[J].中国病原生物学杂志,2017,12(5):385-8,393.
[6]张应烙,尹彩萍.15种中药提取物对几种植物病原菌抑菌活性的初步研究[J].西北农林科技大学学报(自然科学版),2005,33(S1):175-7.
[7]武雪芬,白雁.金银花叶药用成分的提取及抑菌试验研究[J].中成药,2001,23(6):448-9.
[8]钱丽红,陶妍,谢晶.茶多酚对金黄色葡萄球菌和铜绿假单胞菌的抑菌机理[J].微生物学通报,2010,37(11):1628-33.
[9]樊佳,陶榆玮,韩煦等.绿茶抑菌作用及其应用实验探索[J].实验技术与管理,2018,35(6):190-2.
[10]张帅,张舜文,吴芳,等.泛素样蛋白蛋白酶体系统对单耐异烟肼结核分枝杆菌耐药性机制研究[J].中国病原生物学杂志,2017,12(6):489-94.
[11]师伟.中药有效成分抑菌机制研究[J].科技信息,2011(5):37,49.
[12]席清平,唐荣银,王秦芳,等.五倍子在人工口腔中抗菌斑生物膜作用的研究[J].牙体牙髓牙周病学杂志,2004,14(3):148-50.
[13]赵雨,杨菲,张鑫,等.人参生长季根中几种氧化还原酶的活力变化研究[J].辽宁中医杂志,2012,39(8):1586-8.
[14]刘迪.人参皂苷协同抑制HEK-293细胞氧化应激损伤及机制研究[D].吉林大学,2016.
[15]王海南.人参皂苷药理研究进展[J].中国临床药理学与治疗学,2006,11(11):1201-6.
[16]黎阳,张铁军,刘素香,等.人参化学成分和药理研究进展[J].中草药,2009,40(1):164-6.
[17]陈群,刘家昌.人参多糖、黄芪多糖、枸杞多糖的研究进展[J].淮南师范学院学报,2001,3(2):39-41.
[18]李志萍,于秀芳,杭瑚,等.人参对金黄色葡萄球菌的代谢过程促进作用的研究[J].物理化学学报,1995,12(5):468-71.
[19]曹俊岭.人参皂苷Rg1、Rb1、Rd对大肠埃希菌生物热活性的影响[A].中国药学会、河北省人民政府.2008年中国药学会学术年会暨第八届中国药师周论文集[C].中国药学会、河北省人民政府,2008.2771-4.
[20]林锋强.人参皂甙对奶牛金黄色葡萄球菌乳房炎菌苗的免疫佐剂作用[D].浙江大学,2002.
[21]于国东,彭志允.白虎加人参汤治疗下呼吸道多重耐药菌感染临床观察[J].新中医,2012,44(5):31-2.
[22]刘芳芳,张爱华,雷峰杰,等.人参茎叶总皂苷对腐皮镰刀菌的抑制作用及抑菌机理[J].吉林农业大学学报,2018,40(1):85-91.
[23]董春雷.人参皂苷单体及其与其他中药单体联合应用对细菌生物膜抑制作用的研究[D].长春中医药大学,2011.
[24]尹良军,周振旗,绍元,等.盐酸小檗碱、人参皂苷Rb1、黄芩苷及绿原酸对大肠埃希菌和金黄色葡萄球菌的抑菌作用研究[J].医学综述,2016,22(24):4969-72.
[25]刘珏玲,杨伟峰,王毅.细菌生物膜与表面活性剂抗菌的研究进展[J].中国病原生物学杂志,2016,11(9):858-60,附页3.
[26]Lister JL,Horswill AR.Staphylococcus aureus biofilms:recent developments in biofilm dispersal[J].Front Cell Infect Microbial,2014(4):178.
[27]McCartby H,Rudkin JK,Black NS,et al.Methicillin resistance and the biofilm phenotype in Staphylococcus aureus[J].Front Cell Infect Microbial,2015(5):1.
[28]Aka ST,Haji SH.Sub-MIC of antibiotic induced biofilm formation of Pseudomonas aeruginosa in the presence of chlorhexidine[J].Braz J Microbiol,2015,46(1):149-54.
[29]安继红,吕维玲,张永州,等.人参皂苷对金黄色葡萄球菌生物被膜形成的影响[J].山东大学学报(医学版),2018,56(7):14.
[30]Zhang J,Sun Y,Wang Y,et al.Non-antibiotic agent ginsenoside 20(S)-Rh2enhanced the antibacterial effects of ciprofloxacin in vitro and in vivo as a poterntial NorA inhibitor[J].Eur J Pharmacol,2014(740):277-84.收稿日期修回日期
[2]李睿明,雷朝霞.细菌耐药性对抗策略--中药延缓、逆转细菌耐药性,治疗耐药细菌感染的研究[J].医学与哲学(临床决策论坛版),2006,27(8):45-7.
[3]胡晋婷,马萍,陈瑶,等.中药及活性成分对细菌生物膜防治研究近况[J].亚太传统医药,2016,12(24):59-61.
[4]李亚娜,陶庆春.中药抑菌的研究现状及思考[J].国际检验医学杂志,2014,35(2):198-200.
[5]戚健君,王文林,李树德.人参皂干Rg1对NOX3致斯氏狸殖吸虫肝纤维化的影响[J].中国病原生物学杂志,2017,12(5):385-8,393.
[6]张应烙,尹彩萍.15种中药提取物对几种植物病原菌抑菌活性的初步研究[J].西北农林科技大学学报(自然科学版),2005,33(S1):175-7.
[7]武雪芬,白雁.金银花叶药用成分的提取及抑菌试验研究[J].中成药,2001,23(6):448-9.
[8]钱丽红,陶妍,谢晶.茶多酚对金黄色葡萄球菌和铜绿假单胞菌的抑菌机理[J].微生物学通报,2010,37(11):1628-33.
[9]樊佳,陶榆玮,韩煦等.绿茶抑菌作用及其应用实验探索[J].实验技术与管理,2018,35(6):190-2.
[10]张帅,张舜文,吴芳,等.泛素样蛋白蛋白酶体系统对单耐异烟肼结核分枝杆菌耐药性机制研究[J].中国病原生物学杂志,2017,12(6):489-94.
[11]师伟.中药有效成分抑菌机制研究[J].科技信息,2011(5):37,49.
[12]席清平,唐荣银,王秦芳,等.五倍子在人工口腔中抗菌斑生物膜作用的研究[J].牙体牙髓牙周病学杂志,2004,14(3):148-50.
[13]赵雨,杨菲,张鑫,等.人参生长季根中几种氧化还原酶的活力变化研究[J].辽宁中医杂志,2012,39(8):1586-8.
[14]刘迪.人参皂苷协同抑制HEK-293细胞氧化应激损伤及机制研究[D].吉林大学,2016.
[15]王海南.人参皂苷药理研究进展[J].中国临床药理学与治疗学,2006,11(11):1201-6.
[16]黎阳,张铁军,刘素香,等.人参化学成分和药理研究进展[J].中草药,2009,40(1):164-6.
[17]陈群,刘家昌.人参多糖、黄芪多糖、枸杞多糖的研究进展[J].淮南师范学院学报,2001,3(2):39-41.
[18]李志萍,于秀芳,杭瑚,等.人参对金黄色葡萄球菌的代谢过程促进作用的研究[J].物理化学学报,1995,12(5):468-71.
[19]曹俊岭.人参皂苷Rg1、Rb1、Rd对大肠埃希菌生物热活性的影响[A].中国药学会、河北省人民政府.2008年中国药学会学术年会暨第八届中国药师周论文集[C].中国药学会、河北省人民政府,2008.2771-4.
[20]林锋强.人参皂甙对奶牛金黄色葡萄球菌乳房炎菌苗的免疫佐剂作用[D].浙江大学,2002.
[21]于国东,彭志允.白虎加人参汤治疗下呼吸道多重耐药菌感染临床观察[J].新中医,2012,44(5):31-2.
[22]刘芳芳,张爱华,雷峰杰,等.人参茎叶总皂苷对腐皮镰刀菌的抑制作用及抑菌机理[J].吉林农业大学学报,2018,40(1):85-91.
[23]董春雷.人参皂苷单体及其与其他中药单体联合应用对细菌生物膜抑制作用的研究[D].长春中医药大学,2011.
[24]尹良军,周振旗,绍元,等.盐酸小檗碱、人参皂苷Rb1、黄芩苷及绿原酸对大肠埃希菌和金黄色葡萄球菌的抑菌作用研究[J].医学综述,2016,22(24):4969-72.
[25]刘珏玲,杨伟峰,王毅.细菌生物膜与表面活性剂抗菌的研究进展[J].中国病原生物学杂志,2016,11(9):858-60,附页3.
[26]Lister JL,Horswill AR.Staphylococcus aureus biofilms:recent developments in biofilm dispersal[J].Front Cell Infect Microbial,2014(4):178.
[27]McCartby H,Rudkin JK,Black NS,et al.Methicillin resistance and the biofilm phenotype in Staphylococcus aureus[J].Front Cell Infect Microbial,2015(5):1.
[28]Aka ST,Haji SH.Sub-MIC of antibiotic induced biofilm formation of Pseudomonas aeruginosa in the presence of chlorhexidine[J].Braz J Microbiol,2015,46(1):149-54.
[29]安继红,吕维玲,张永州,等.人参皂苷对金黄色葡萄球菌生物被膜形成的影响[J].山东大学学报(医学版),2018,56(7):14.
[30]Zhang J,Sun Y,Wang Y,et al.Non-antibiotic agent ginsenoside 20(S)-Rh2enhanced the antibacterial effects of ciprofloxacin in vitro and in vivo as a poterntial NorA inhibitor[J].Eur J Pharmacol,2014(740):277-84.收稿日期修回日期