VEGF基因沉默脂肪间充质干细胞对人MKN45胃癌细胞生长的影响
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摘要
目的研究VEGF基因沉默ADSCs对人MKN45胃癌细胞生长的影响。方法选取2017年10月～2018年10月在本动物实验中心展开随机对照动物实验,分为空白对照组、阴性对照组及VEGF siRNA组,siRNA转染后使用RT-PCR方法检测细胞VEFG表达情况,利用CD34免疫组织化学法染色检测胃癌组织血管生成状况。结果在VEGF表达方面,VEGF siRNA组VEGF表达明显下降,差异有统计学意义(P<0.05);在基因转染后胃癌细胞生长情况方面,VEGF siRNA组肿瘤重量明显更低,差异有统计学意义(P<0.05);在MVD情况方面,VEGF siRNA组MVD视野明显更低,差异有统计学意义(P<0.05)。结论VEGF基因沉默ADSCs后VEGF明显降低,混合MKN45胃癌细胞后接种未影响胃癌生长和血管形成。
Objective To study the effect of ADSCs silenced by vascular endothelial growth factor(VEGF gene on the growth of human MKN45 gastric cancer cells. Methods Randomized controlled animal experiments were conducted in our animal experimental center from October 2017 to October 2018.The animals were divided into blank control group, negative control group and VEGF siRNA group. After siRNA transfection,VEFG expression was detected by RT-PCR,and angiogenesis was detected by CD34 immunohistochemical staining.Results The expression of vascular endothelial growth factor was significantly decreased in the group of vascular endothelial growth factor siRNA(P<0.05);the growth of gastric cancer cells after gene transfection was significantly lower in the group of vascular endothelial growth factor siRNA(P<0.05);and in the case of MVD, the visual field of MVD in the group of vascular endothelial growth factor siRNA was significantly lower(P<0.05). Conclusion After the silencing of ADSCs, the expression of vascular endothelial growth factor was significantly decreased. Mixed MKN45 gastric cancer cells did not affect the growth and angiogenesis of gastric cancer.
Objective To study the effect of ADSCs silenced by vascular endothelial growth factor(VEGF gene on the growth of human MKN45 gastric cancer cells. Methods Randomized controlled animal experiments were conducted in our animal experimental center from October 2017 to October 2018.The animals were divided into blank control group, negative control group and VEGF siRNA group. After siRNA transfection,VEFG expression was detected by RT-PCR,and angiogenesis was detected by CD34 immunohistochemical staining.Results The expression of vascular endothelial growth factor was significantly decreased in the group of vascular endothelial growth factor siRNA(P<0.05);the growth of gastric cancer cells after gene transfection was significantly lower in the group of vascular endothelial growth factor siRNA(P<0.05);and in the case of MVD, the visual field of MVD in the group of vascular endothelial growth factor siRNA was significantly lower(P<0.05). Conclusion After the silencing of ADSCs, the expression of vascular endothelial growth factor was significantly decreased. Mixed MKN45 gastric cancer cells did not affect the growth and angiogenesis of gastric cancer.
引文
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[5]郭伟洪,陈昭宇,陈豪,等.丝胶蛋白通过自噬通路调控人胃癌MKN45细胞的增殖[J].南方医科大学学报,2018,38(2):148-154.