移栽基质对白芨组培苗生长的影响
|
摘要
为了筛选适宜白芨组培苗生长的栽培基质,选用泥炭土、玉米芯、珍珠岩和蛭石为原料,混合配制成4种不同的栽培基质,探讨栽培基质对白芨组培苗成活率、植株生长和根系生长的影响.结果表明,玉米芯可以用作白芨组培苗移栽基质.在蛭石、堆肥土与玉米芯体积比为1∶2∶1的基质中白芨组培苗的成活率最高,6个月后高达62.5%,其生长情况也最好,株高达12.03 cm,根长为4.97 cm,根数为5.69条.
In order to screen suitable substrate for plantlets of Bletilla striata,four raw materials were mixed by peat soil,corncob,perlite and vermiculite,to discuss the effect of four mixed substrates on transplanting survival rate and growth of Bletilla striata in vitro.The results showed that corncob could be used as cultural substrate for Bletilla striata. The transplanting survival rate of Bletilla striata was highest in the mixed substrate with a volume ratio of vermiculite,compost soil and corn cob slag(1∶2∶1),which was 62.5% after six months;its growth condition was best,and its height reached 12.03 cm,root length was 4.97 cm and root number was 5.69.
In order to screen suitable substrate for plantlets of Bletilla striata,four raw materials were mixed by peat soil,corncob,perlite and vermiculite,to discuss the effect of four mixed substrates on transplanting survival rate and growth of Bletilla striata in vitro.The results showed that corncob could be used as cultural substrate for Bletilla striata. The transplanting survival rate of Bletilla striata was highest in the mixed substrate with a volume ratio of vermiculite,compost soil and corn cob slag(1∶2∶1),which was 62.5% after six months;its growth condition was best,and its height reached 12.03 cm,root length was 4.97 cm and root number was 5.69.
引文
[1] 中国科学院中国植物志编辑委员会.中国植物志(第18卷)[M].北京:科学出版社,1999.China Flora Editorial Board of Chinese Academy of Sciences.China flora(volumes 18)[M].Bejing:Science Press,1999.
[2] 倪子轶,刘群录.白芨的栽培及景观应用探讨[J].现代农业科技,2018(18):136-137.Ni Z Y,Liu Q L.Planting and discussion on landscape application of Bletilla striata[J].Modern Agricultural Science and Technology,2018(18):136-137.
[3] 中国药典委员会.中华人民共和国药典(第一部)[M].北京:人民卫生出版社,1995.Chinese Pharmacopoeia Commission.Chinese pharmacopoeia(part one)[M].Bejing:People's Medical Publishing House,1995.
[4] 罗磊,杨帆,王瑶,等.白芨多糖的实验及应用研究[J].湖北中医杂志,2016,38(11):69-72.Luo L,Yang F,Wang Y,et al.The applied research and experiments of Bletilla polysaccharide[J].Hubei Journal of Traditional Chinese Medicine,2016,38(11):69-72.
[5] 陈灿,陈海霞.白芨繁殖研究进展[J].湖南农业科学,2015(5):135-137,141.Chen C,Chen H X.Research progress in tissue culture of Bletilla striata[J].Hunan Agricultural Sciences ,2015(5):135-137,141.
[6] 姜丽琼,李文俊,肖前刚,等.白芨炼苗与林下种植实用技术[J].林业科技通讯,2017(6):62-64.Jiang L Q,Li W J,Xiao Q G,et al.The practical techniques of transplanting and underwood planting of Bletilla striata[J].Forest Science and Technology,2017(6):62-64.
[7] 王君厚.近50年来我国沙化土地动态变化分析[J].林业资源管理,2008(2):23-27.Wang J H.Brief analysis on dynamic changes of sandified land areas in China since 1960s[J].Forest Resources Management,2008(2):23-27.
[8] 张燕,黎斌,李思锋.不同培养基上白芨的种子萌发与幼苗形态发生[J].西北植物学报,2009,29(8):1 584-1 589.Zhang Y,Li B,Li S F.Seed germination and seeding morphogenesis of Bletilla striata under the different culture medium[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(8):1 584-1 589.
[9] 邹娜,李意,连芳青.优良观赏药用地被植物-白芨组织培养及快速繁殖研究[J].江西农业大学学报,2013,35(5):950-955.Zou N,Li Y,Lian F Q.Tissue culture and rapid propagation of excellent ornamental and medicinal ground cover plants-Bletilla striata[J].Acta Agriculturae Universitatis Jiangxiensis,2013,35(5):950-955.
[10] 马丹.白芨快速繁殖体系的建立[D].南昌:南昌大学,2016.Ma D.The establishment of Bletilla striata rapid propagation system[D].Nanchang:Nanchang Universiy,2016.
[11] 余朝秀,李枝林,王玉英.野生白芨组培快繁技术研究[J].西南农业大学学报(自然科学版),2005,27(5):601-604.Yu Z X,Li Z L,Wang Y Y.Tissue culture and rapid propagation of wild Bletilla[J].Journal of Southwest Agricultural University(Natural Science),2005,27(5):601-604.
[12] 曹云娥,张学忠,杨世宏,等.不同农业有机废料发酵基质栽培番茄试验[J].北方园艺,2012(10):24-28.Cao Y E,Zhang X Z,Yang S H,et al.Study on the cultivation of tomatoes with different organic wasted as eco-organic soilless cultivation substrate[J].Northern Horticulture,2012(10):24-28.
[13] 宋志刚,余宏军,蒋卫杰,等.稻草复合基质对番茄育苗效果的影响[J].中国蔬菜,2013(14):72-77.Song Z G,Yu H J,Jiang W J,et al.Effect of straw compound substrate on tomato seedling culture[J].China Vegetables,2013(14):72-77.
[14] 徐文俊,程智慧,孟焕文,等.农业废弃有机物基质配方对番茄生长及产量的影响[J].西北农林科技大学学报(自然科学版),2012,40(4):127-133.Xu W J,Cheng Z H,Meng H W,et al.Influence of the formula of organic waste substrate from crop production on growth and yield of tomato[J].Journal of Northwest A&F University((Natural Science Edition),2012,40(4):127-133.
[15] Baran A,Cayci G,Kütük C,et al.Composted grape marcas growing medium for hypostases (Hypostases phyllostagya)[J].Bioresource Technology,2001,78(1):103-106.
[16] 吴尚军,郭永杰,魏金康.杏鲍菇栽培基质配方筛选试验[J].北京农业,2009(27):43-45.Wu S J,Guo Y J,Wei J K.Selection of culture medium ratio for pleurotus eryngii planting [J].Beijing Agriculture,2009(27):43-45.
[17] 张硕,余宏军,蒋卫杰.发酵玉米芯或甘蔗渣基质的黄瓜育苗效果[J].农业工程学报,2015,31(11):236-242.Zhang S,Yu H J,Jiang W J.Seedling effects of corncob and bagasse composting substrates in cucumber[J].Transactions of the Chinese Society of Agricultural Engineering,2015,31(11):236-242.
[18] 张云,刘慧英,张倩云,等.玉米芯发酵基质在加工番茄育苗上的应用[J].黑龙江农业科学,2014(7):80-83.Zhang Y,Liu H Y,Zhang Q Y,et al.Application of corncob fermentation substrates in the processing of tomato seedling[J].Heilongjiang Agricultural Sciences,2014(7):80-83.
[19] 高雷,赵卫国,莫东发.不同栽培基质对红掌组培出瓶苗生长的影响[J].北方园艺,2008(7):164-166.Gao L,Zhao W G,Mo D F.The effects of different nutrition substitude on the growth of the tissue culture Anthurium adndraenum seedling[J].Northern Horticulture,2008(7):164-166.
[20] 陈海霞,赵红英,张虓峰.基质种类对八仙花组培苗瓶外生根的影响[J].湖南生态科学学报,2017,4(4):9-13.Chen H X,Zhao H Y,Zhang X F.Effect of matrix type on plantlet rooting out of test-tube of Hydrangea macrophylla[J].Journal of Hunan Ecological Science,2017,4(4):9-13.
[21] 曹婧,李婷,包彩云,等.白芨组培苗移栽驯化技术研究[J].内江师范学院学报,2015,30(4):39-41.Cao J,Li T,Bao C Y,et al.On transplanting technology of tissue culture seedlings of Bletilla striata[J].Journal of Neijiang Normal University,2015,30(4):39-41.
[22] 袁宁,何俊蓉,何锐,等.白芨组培快繁育苗技术研究[J].西南农业学报,2009,22(3):781-785.Yuan N,He J R,He R,et al.Tissue culture and rapid propagation of Bletilla striata[J].Southwest China Journal of Agricultural Sciences,2009,22(3):781-785.
[2] 倪子轶,刘群录.白芨的栽培及景观应用探讨[J].现代农业科技,2018(18):136-137.Ni Z Y,Liu Q L.Planting and discussion on landscape application of Bletilla striata[J].Modern Agricultural Science and Technology,2018(18):136-137.
[3] 中国药典委员会.中华人民共和国药典(第一部)[M].北京:人民卫生出版社,1995.Chinese Pharmacopoeia Commission.Chinese pharmacopoeia(part one)[M].Bejing:People's Medical Publishing House,1995.
[4] 罗磊,杨帆,王瑶,等.白芨多糖的实验及应用研究[J].湖北中医杂志,2016,38(11):69-72.Luo L,Yang F,Wang Y,et al.The applied research and experiments of Bletilla polysaccharide[J].Hubei Journal of Traditional Chinese Medicine,2016,38(11):69-72.
[5] 陈灿,陈海霞.白芨繁殖研究进展[J].湖南农业科学,2015(5):135-137,141.Chen C,Chen H X.Research progress in tissue culture of Bletilla striata[J].Hunan Agricultural Sciences ,2015(5):135-137,141.
[6] 姜丽琼,李文俊,肖前刚,等.白芨炼苗与林下种植实用技术[J].林业科技通讯,2017(6):62-64.Jiang L Q,Li W J,Xiao Q G,et al.The practical techniques of transplanting and underwood planting of Bletilla striata[J].Forest Science and Technology,2017(6):62-64.
[7] 王君厚.近50年来我国沙化土地动态变化分析[J].林业资源管理,2008(2):23-27.Wang J H.Brief analysis on dynamic changes of sandified land areas in China since 1960s[J].Forest Resources Management,2008(2):23-27.
[8] 张燕,黎斌,李思锋.不同培养基上白芨的种子萌发与幼苗形态发生[J].西北植物学报,2009,29(8):1 584-1 589.Zhang Y,Li B,Li S F.Seed germination and seeding morphogenesis of Bletilla striata under the different culture medium[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(8):1 584-1 589.
[9] 邹娜,李意,连芳青.优良观赏药用地被植物-白芨组织培养及快速繁殖研究[J].江西农业大学学报,2013,35(5):950-955.Zou N,Li Y,Lian F Q.Tissue culture and rapid propagation of excellent ornamental and medicinal ground cover plants-Bletilla striata[J].Acta Agriculturae Universitatis Jiangxiensis,2013,35(5):950-955.
[10] 马丹.白芨快速繁殖体系的建立[D].南昌:南昌大学,2016.Ma D.The establishment of Bletilla striata rapid propagation system[D].Nanchang:Nanchang Universiy,2016.
[11] 余朝秀,李枝林,王玉英.野生白芨组培快繁技术研究[J].西南农业大学学报(自然科学版),2005,27(5):601-604.Yu Z X,Li Z L,Wang Y Y.Tissue culture and rapid propagation of wild Bletilla[J].Journal of Southwest Agricultural University(Natural Science),2005,27(5):601-604.
[12] 曹云娥,张学忠,杨世宏,等.不同农业有机废料发酵基质栽培番茄试验[J].北方园艺,2012(10):24-28.Cao Y E,Zhang X Z,Yang S H,et al.Study on the cultivation of tomatoes with different organic wasted as eco-organic soilless cultivation substrate[J].Northern Horticulture,2012(10):24-28.
[13] 宋志刚,余宏军,蒋卫杰,等.稻草复合基质对番茄育苗效果的影响[J].中国蔬菜,2013(14):72-77.Song Z G,Yu H J,Jiang W J,et al.Effect of straw compound substrate on tomato seedling culture[J].China Vegetables,2013(14):72-77.
[14] 徐文俊,程智慧,孟焕文,等.农业废弃有机物基质配方对番茄生长及产量的影响[J].西北农林科技大学学报(自然科学版),2012,40(4):127-133.Xu W J,Cheng Z H,Meng H W,et al.Influence of the formula of organic waste substrate from crop production on growth and yield of tomato[J].Journal of Northwest A&F University((Natural Science Edition),2012,40(4):127-133.
[15] Baran A,Cayci G,Kütük C,et al.Composted grape marcas growing medium for hypostases (Hypostases phyllostagya)[J].Bioresource Technology,2001,78(1):103-106.
[16] 吴尚军,郭永杰,魏金康.杏鲍菇栽培基质配方筛选试验[J].北京农业,2009(27):43-45.Wu S J,Guo Y J,Wei J K.Selection of culture medium ratio for pleurotus eryngii planting [J].Beijing Agriculture,2009(27):43-45.
[17] 张硕,余宏军,蒋卫杰.发酵玉米芯或甘蔗渣基质的黄瓜育苗效果[J].农业工程学报,2015,31(11):236-242.Zhang S,Yu H J,Jiang W J.Seedling effects of corncob and bagasse composting substrates in cucumber[J].Transactions of the Chinese Society of Agricultural Engineering,2015,31(11):236-242.
[18] 张云,刘慧英,张倩云,等.玉米芯发酵基质在加工番茄育苗上的应用[J].黑龙江农业科学,2014(7):80-83.Zhang Y,Liu H Y,Zhang Q Y,et al.Application of corncob fermentation substrates in the processing of tomato seedling[J].Heilongjiang Agricultural Sciences,2014(7):80-83.
[19] 高雷,赵卫国,莫东发.不同栽培基质对红掌组培出瓶苗生长的影响[J].北方园艺,2008(7):164-166.Gao L,Zhao W G,Mo D F.The effects of different nutrition substitude on the growth of the tissue culture Anthurium adndraenum seedling[J].Northern Horticulture,2008(7):164-166.
[20] 陈海霞,赵红英,张虓峰.基质种类对八仙花组培苗瓶外生根的影响[J].湖南生态科学学报,2017,4(4):9-13.Chen H X,Zhao H Y,Zhang X F.Effect of matrix type on plantlet rooting out of test-tube of Hydrangea macrophylla[J].Journal of Hunan Ecological Science,2017,4(4):9-13.
[21] 曹婧,李婷,包彩云,等.白芨组培苗移栽驯化技术研究[J].内江师范学院学报,2015,30(4):39-41.Cao J,Li T,Bao C Y,et al.On transplanting technology of tissue culture seedlings of Bletilla striata[J].Journal of Neijiang Normal University,2015,30(4):39-41.
[22] 袁宁,何俊蓉,何锐,等.白芨组培快繁育苗技术研究[J].西南农业学报,2009,22(3):781-785.Yuan N,He J R,He R,et al.Tissue culture and rapid propagation of Bletilla striata[J].Southwest China Journal of Agricultural Sciences,2009,22(3):781-785.