滴灌施用噻虫胺防治柑橘木虱研究
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摘要
【目的】明确滴灌施药防治柑橘木虱Diaphorina citri Kuwayama的可行性,为田间控制柑橘黄龙病传播提供新的手段。【方法】通过室内模拟滴灌施用噻虫胺,测定了噻虫胺在土壤中的分布以及柑橘叶片中含量,研究了滴灌施药对柑橘木虱防治效果。通过田间试验,检测分析了柑橘叶片中噻虫胺含量。【结果】在室内条件下,一次滴灌施药对柑橘木虱的防治效果可长达4个月;噻虫胺在土壤中以滴头为起始点在土壤中呈半圆形迁移分布,当柑橘叶片中噻虫胺质量分数大于0.64 mg·kg–1时,对柑橘木虱的防治效果均高于80%。田间试验表明,在药后7～63 d柑橘上部叶片中噻虫胺的质量分数大于0.64 mg·kg–1。【结论】滴灌施用噻虫胺能有效防治柑橘木虱,是田间控制柑橘黄龙病传播的有效手段。
【Objective】 To study the control effect of clothianidin on citrus psyllid(Diaphorina citri) under drip irrigation, and provide a new way for controlling Huanglongbing(HLB) spread. 【Method】 An indoor simulation drip irrigation experiment was conducted for clothianidin. The distribution of clothianidin in soil and the content in citrus leaves were tested, and the clothianidin efficacy was studied under laboratory condition.The content of clothianidin in citrus leaves was detected and analyzed in field experiment. 【Result】The control effect of single drip irrigation could be as long as four months in greenhouse. The migration distribution of clothianidin was a semicircle with a drop head as a starting point in soil, and the control effects of D. citri were more than 80% when clothianidin contents of citrus leaves were greater than 0.64 mg·kg–1. Under field condition, the contents of clothianidin in upper leaves were also greater than 0.64 mg·kg–1 from 7 to 63 days after applying clothianidin. 【Conclusion】 Clothianidin application through a drip irrigation system exhibits excellent efficacy against citrus psyllid, which provides an effective measure for prevention and control of HLB.
【Objective】 To study the control effect of clothianidin on citrus psyllid(Diaphorina citri) under drip irrigation, and provide a new way for controlling Huanglongbing(HLB) spread. 【Method】 An indoor simulation drip irrigation experiment was conducted for clothianidin. The distribution of clothianidin in soil and the content in citrus leaves were tested, and the clothianidin efficacy was studied under laboratory condition.The content of clothianidin in citrus leaves was detected and analyzed in field experiment. 【Result】The control effect of single drip irrigation could be as long as four months in greenhouse. The migration distribution of clothianidin was a semicircle with a drop head as a starting point in soil, and the control effects of D. citri were more than 80% when clothianidin contents of citrus leaves were greater than 0.64 mg·kg–1. Under field condition, the contents of clothianidin in upper leaves were also greater than 0.64 mg·kg–1 from 7 to 63 days after applying clothianidin. 【Conclusion】 Clothianidin application through a drip irrigation system exhibits excellent efficacy against citrus psyllid, which provides an effective measure for prevention and control of HLB.
引文
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[2]张培,关磊,刘蕤,等.砂糖橘红鼻子果与韧皮部杆菌侵染相关性研究[J].热带作物学报,2011,32(4):738-742.
[3]BOINA D R,BLOOMQUIST J R.Chemical control of the Asian citrus psyllid and of Huanglongbing disease in citrus[J].Pest Manag Sci,2015,71(6):808-823.
[4]XU M R,LIANG M D,CHEN J C,et al.Preliminary research on soil conditioner mediated citrus Huanglongbing mitigation in the field in Guangdong,China[J].Eur JPlant Pathol,2013,137(2):283-293.
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[8]余继华,黄振东,张敏荣,等.亚洲柑橘木虱带菌率的周年变化动态[J].浙江大学学报(农业与生命科学版),2017,43(1):89-94.
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[11]彭良志,淳长品,江才伦,等.滴灌施肥对‘特罗维它’甜橙生长结果的影响[J].园艺学报,2011,38(1):1-6.
[12]AMMAR E,WALTER A J,HALL D G.New excisedleaf assay method to test inoculativity of Asian citrus psyllid(Hemiptera:Psyllidae)with CandidatusLiberibacter asiaticus associated with citrus Huanglongbing disease[J].J Econ Entomol,2013,106(1):25-35.
[13]孙桂春.试论柑桔木虱的分布消长及综合防治技术[J].中国南方果树,1999,28(6):17-18.
[14]HALL D G,RICHARDSON M L,AMMAR E,et al.Asian citrus psyllid,Diaphorina citri,vector of citrus Huanglongbing disease[J].Entomol Exp Appl,2013,146(2):207-223.
[15]许长藩,夏雨华,柯冲.柑桔木虱生物学特性及防治研究[J].植物保护学报,1994,21(1):53-56.
[16]程保平,赵弘巍,彭埃天,等.柑橘黄龙病的传播介体:柑橘木虱在广东果园的发生调查[J].植物保护,2016,42(1):189-192.
[17]吴春笃,王光亮,沈林生.久效磷在悬铃木植株体内动态分布的研究[J].农业工程学报,1994,10(4):96-101.
[18]田鹏鹏.树干注射吡虫啉在树体内的吸收传导分布研究[D].杨凌:西北农林科技大学,2008.
[19]DELéTAGE-GRANDON C,CHOLLET J F,FAUCH-ER M,et al.Carrier-mediated uptake and phloem systemy of a 350-dalton chlorinated xenobiotic with an alpha-amino acid function[J].Plant Physiology,2001,125(4):1620-1632.
[20]BROMILOW R H,CHAMBERLAIN K.The herbicide glyphosate and related molecules:Physicochemical and structural factors determining their mobility in phloem[J].Pest Manag Sci,2000,56(4):368-373.
[21]GHIDIU G,KUHAR T,PALUMBO J,et al.Drip chemigation of insecticides as a pest management tool in vegetable production[J].J Integr Pest Manag,2012,3(3):1-5.
[22]GHIDIU G M.Integrated pest management and pest control:Current and future tactics[M].InTech Open Access Publisher,2012:173-190.
[23]WANG X,ZHU H,REDING M E,et al.Delivery of chemical and microbial pesticides through drip irrigation systems[J].Appl Eng Agric,2009,25(6):883-893.
[24]张明媚,孙克,吴鸿飞,等.噻虫胺的合成[J].农药,2010,49(2):94-96.
[25]LANGDON K W,ROGERS M E.Neonicotinoid-induced mortality of Diaphorina citri(Hemiptera:Liviidae)is affected by route of exposure[J].J Econ Entomol,,2017,110(5):2229-2234.
[26]陈红,魏盛禄,陈伟,等.30%噻虫胺除虫脲悬浮剂防治柑橘木虱的田间药效试验[J].农业与技术,2017,37(10):48.
[27]ICHINOSE K,BANG D V,TUAN D H,et al.Effective use of neonicotinoids for protection of citrus seedlings from invasion by Diaphorina citri(Hemiptera:Psyllidae)[J].J Econ Entomol,2010,103:127-135.
[28]MOFFAT C,BUCKLAND S T,SAMSON A J,et al.Neonicotinoids target distinct nicotinic acetylcholine receptors and neurons,leading to differential risks to bumblebees[J].Sci Rep,2016.doi:10.1038/srep24764(2016).
[2]张培,关磊,刘蕤,等.砂糖橘红鼻子果与韧皮部杆菌侵染相关性研究[J].热带作物学报,2011,32(4):738-742.
[3]BOINA D R,BLOOMQUIST J R.Chemical control of the Asian citrus psyllid and of Huanglongbing disease in citrus[J].Pest Manag Sci,2015,71(6):808-823.
[4]XU M R,LIANG M D,CHEN J C,et al.Preliminary research on soil conditioner mediated citrus Huanglongbing mitigation in the field in Guangdong,China[J].Eur JPlant Pathol,2013,137(2):283-293.
[5]BOVE J M.Huanglongbing:A destructive,newly-emerging,century-old disease of citrus[J].J Plant Pathol,2006,88:7-37.
[6]CEN Y J,YANG C L,HOLFORD P,et al.Feeding behaviour of the Asiatic citrus psyllid,Diaphorina citri,on healthy and Huanglongbing-infected citrus[J].Entomol Exp Appl,2012,143(1):13-22.
[7]WU F N,HUANG J Q,XU M R,et al.Host and environmental factors influencing“Candidatus Liberibacter asiaticus”acquisition in Diaphorina citri[J/OL].Pest Manag Sci,2018.(2018-05-03).https://doi.org/10.1002/ps.5060.doi:10.1002/ps.5060.
[8]余继华,黄振东,张敏荣,等.亚洲柑橘木虱带菌率的周年变化动态[J].浙江大学学报(农业与生命科学版),2017,43(1):89-94.
[9]AJWA H A,TROUT T,MUELLER J,et al.Application of alternative fumigants through drip irrigation systems[J].Phytopathology,2002,92(12):1349-1355.
[10]KUHAR T P,WALGENBACH J F,DOUGHTY H B.Control of Helicoverpa zea in tomatoes with chlorantraniliprole applied through drip chemigation[J/OL].Plant Health Progress,2010.(2010-04-07).http://172.16.1.72/sub/php/research/2010/tomato/tomato.asp.doi:10.1094/PHP-2009-0407-01-RS.
[11]彭良志,淳长品,江才伦,等.滴灌施肥对‘特罗维它’甜橙生长结果的影响[J].园艺学报,2011,38(1):1-6.
[12]AMMAR E,WALTER A J,HALL D G.New excisedleaf assay method to test inoculativity of Asian citrus psyllid(Hemiptera:Psyllidae)with CandidatusLiberibacter asiaticus associated with citrus Huanglongbing disease[J].J Econ Entomol,2013,106(1):25-35.
[13]孙桂春.试论柑桔木虱的分布消长及综合防治技术[J].中国南方果树,1999,28(6):17-18.
[14]HALL D G,RICHARDSON M L,AMMAR E,et al.Asian citrus psyllid,Diaphorina citri,vector of citrus Huanglongbing disease[J].Entomol Exp Appl,2013,146(2):207-223.
[15]许长藩,夏雨华,柯冲.柑桔木虱生物学特性及防治研究[J].植物保护学报,1994,21(1):53-56.
[16]程保平,赵弘巍,彭埃天,等.柑橘黄龙病的传播介体:柑橘木虱在广东果园的发生调查[J].植物保护,2016,42(1):189-192.
[17]吴春笃,王光亮,沈林生.久效磷在悬铃木植株体内动态分布的研究[J].农业工程学报,1994,10(4):96-101.
[18]田鹏鹏.树干注射吡虫啉在树体内的吸收传导分布研究[D].杨凌:西北农林科技大学,2008.
[19]DELéTAGE-GRANDON C,CHOLLET J F,FAUCH-ER M,et al.Carrier-mediated uptake and phloem systemy of a 350-dalton chlorinated xenobiotic with an alpha-amino acid function[J].Plant Physiology,2001,125(4):1620-1632.
[20]BROMILOW R H,CHAMBERLAIN K.The herbicide glyphosate and related molecules:Physicochemical and structural factors determining their mobility in phloem[J].Pest Manag Sci,2000,56(4):368-373.
[21]GHIDIU G,KUHAR T,PALUMBO J,et al.Drip chemigation of insecticides as a pest management tool in vegetable production[J].J Integr Pest Manag,2012,3(3):1-5.
[22]GHIDIU G M.Integrated pest management and pest control:Current and future tactics[M].InTech Open Access Publisher,2012:173-190.
[23]WANG X,ZHU H,REDING M E,et al.Delivery of chemical and microbial pesticides through drip irrigation systems[J].Appl Eng Agric,2009,25(6):883-893.
[24]张明媚,孙克,吴鸿飞,等.噻虫胺的合成[J].农药,2010,49(2):94-96.
[25]LANGDON K W,ROGERS M E.Neonicotinoid-induced mortality of Diaphorina citri(Hemiptera:Liviidae)is affected by route of exposure[J].J Econ Entomol,,2017,110(5):2229-2234.
[26]陈红,魏盛禄,陈伟,等.30%噻虫胺除虫脲悬浮剂防治柑橘木虱的田间药效试验[J].农业与技术,2017,37(10):48.
[27]ICHINOSE K,BANG D V,TUAN D H,et al.Effective use of neonicotinoids for protection of citrus seedlings from invasion by Diaphorina citri(Hemiptera:Psyllidae)[J].J Econ Entomol,2010,103:127-135.
[28]MOFFAT C,BUCKLAND S T,SAMSON A J,et al.Neonicotinoids target distinct nicotinic acetylcholine receptors and neurons,leading to differential risks to bumblebees[J].Sci Rep,2016.doi:10.1038/srep24764(2016).