微波萃取技术提取谷精草中总黄酮的工艺优化
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摘要
采用微波萃取技术对谷精草中总黄酮类化合物进行提取,并利用紫外分光光度法测定总黄酮的含量,将测得的总黄酮含量作为比较分析的标准。本实验分析了时间、料液比、温度、乙醇浓度对总黄酮提取率的影响,根据单因素实验的分析结果,以L9(34)进行正交实验,以期得到一个最佳的微波萃取工艺。结果表明,各因素作用程度为:料液比>乙醇浓度>温度>时间,其中,料液比对实验结果的影响最为突出,乙醇浓度和温度的影响略轻,时间的影响最小;正交实验结果表明提取时间70min,温度70℃,乙醇浓度40%,料液比1:15为最佳提取工艺。
The total flavonoids from Eriocaulon buergerianum was extracted by microwave extraction technique,the content of total flavone was determined by UV spectrophotometry,and the content of total flavonoids was measured as a comparative analysis of standard. The experiment analyzed the time,solid-liquid ratio,temperature,ethanol concentration. According to the single factor experiment results,adopted L9( 34) orthogonal test experiment to optimize the extraction process of total flavonoids. The results showed that the effect degree of each factor is material to liquid > ethanol concentration > temperature > time,feed the effect of solid-liquid ratio on the experimental results of the most prominent effects of ethanol concentration and temperature slightly,time has little effect. The results of orthogonal experiment analysis shows that the extraction time 70 min,temperature 70℃,40%ethanol concentration ratio of material to liquid 1: 15 as the best extraction process.
The total flavonoids from Eriocaulon buergerianum was extracted by microwave extraction technique,the content of total flavone was determined by UV spectrophotometry,and the content of total flavonoids was measured as a comparative analysis of standard. The experiment analyzed the time,solid-liquid ratio,temperature,ethanol concentration. According to the single factor experiment results,adopted L9( 34) orthogonal test experiment to optimize the extraction process of total flavonoids. The results showed that the effect degree of each factor is material to liquid > ethanol concentration > temperature > time,feed the effect of solid-liquid ratio on the experimental results of the most prominent effects of ethanol concentration and temperature slightly,time has little effect. The results of orthogonal experiment analysis shows that the extraction time 70 min,temperature 70℃,40%ethanol concentration ratio of material to liquid 1: 15 as the best extraction process.
引文
[1]缪珠雷,田春雨,程磊,等.《本草纲目》谷精草的原植物考证[J].上海中医药杂志,2004,38(5):60.
[2]杨文晨,刘惠,倪士峰,等.谷精草科药学研究概况[J].中医药学报,2009,37(04):92-93.
[3]黄和,黄涛阳,陈龙浩,等.谷精草科研究综述[J].中国医药学,2014,9(05):676-678.
[4]杨卫平主编.临床常用中药手册[M].贵州科技出版社,2001:104.
[5]袁建梅,尚学芳,汪应灵,等.谷精草总黄酮提取及对羟自由基清除作用研究[J].时珍国医国药.2010,21(04).894-895.
[6]王艳芳,王新华,朱宇桐.槲皮素药理研究进展[J].天然产物研究与开发,2003.15(2):171.
[7]龚金炎,张英,吴晓琴.黄酮类化合物抗病毒活性的研究进展[J].中草药,2008,39(4):623-627.
[8]张军,高丽萍,魏虎来.洋芹素,槲皮素和万寿菊素衍生物生物体外抗氧化作用研究[J].中药药理与临床,2013,11(4):189-190.
[9]严敏,唐筱露.黄酮类化合物抗病毒作用研究概况[J].亚太传统医药,2009,5(09):149-150.
[10]王慧.黄酮类化合物生物活性的研究进展[J].食品与药品.2010(09):347-350.
[11]贺敏,李雅萍,黄仁彬.抗糖尿病黄酮类活性成分的研究进展[J].中国医院药学杂志,2009(17):1481-1483.
[12]冯丽娟,陈芳,孙智勇,等.超声波法提取谷精草中总黄酮的条件优化[J].江西农业学报,2014,26(06):84-86.
[13]袁建梅,尚学芳,耿明江,等.谷精草黄酮类化合物的提取工艺研究[J].食品工业科技,2010,31(6):239-241.
[14]陈亚妮,张军民.微波萃取技术研究进展[J].应用化工,2010,39(2):270-272.
[2]杨文晨,刘惠,倪士峰,等.谷精草科药学研究概况[J].中医药学报,2009,37(04):92-93.
[3]黄和,黄涛阳,陈龙浩,等.谷精草科研究综述[J].中国医药学,2014,9(05):676-678.
[4]杨卫平主编.临床常用中药手册[M].贵州科技出版社,2001:104.
[5]袁建梅,尚学芳,汪应灵,等.谷精草总黄酮提取及对羟自由基清除作用研究[J].时珍国医国药.2010,21(04).894-895.
[6]王艳芳,王新华,朱宇桐.槲皮素药理研究进展[J].天然产物研究与开发,2003.15(2):171.
[7]龚金炎,张英,吴晓琴.黄酮类化合物抗病毒活性的研究进展[J].中草药,2008,39(4):623-627.
[8]张军,高丽萍,魏虎来.洋芹素,槲皮素和万寿菊素衍生物生物体外抗氧化作用研究[J].中药药理与临床,2013,11(4):189-190.
[9]严敏,唐筱露.黄酮类化合物抗病毒作用研究概况[J].亚太传统医药,2009,5(09):149-150.
[10]王慧.黄酮类化合物生物活性的研究进展[J].食品与药品.2010(09):347-350.
[11]贺敏,李雅萍,黄仁彬.抗糖尿病黄酮类活性成分的研究进展[J].中国医院药学杂志,2009(17):1481-1483.
[12]冯丽娟,陈芳,孙智勇,等.超声波法提取谷精草中总黄酮的条件优化[J].江西农业学报,2014,26(06):84-86.
[13]袁建梅,尚学芳,耿明江,等.谷精草黄酮类化合物的提取工艺研究[J].食品工业科技,2010,31(6):239-241.
[14]陈亚妮,张军民.微波萃取技术研究进展[J].应用化工,2010,39(2):270-272.