HPLC同时测定蒙药阿那日-4中3种成分的含量
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摘要
目的建立HPLC同时测定蒙药阿那日-4中鞣花酸、桂皮醛和胡椒碱3种活性成分含量的方法。方法采用Agilent ZORBAX Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸水溶液为流动相,梯度洗脱,流速1.0 ml/min,柱温30℃,检测波长271 nm。结果供试品溶液在24 h内稳定,鞣花酸、桂皮醛、胡椒碱质量浓度分别在0.050～0.500,0.030～0.300,0.030～0.300 mg/ml范围内与峰面积呈良好的线性关系,相关系数分别为0.9994,0.9995,0.9997;平均加样回收率(n=6)分别为101.68%,100.94%,98.55%,RSD分别为1.806%,1.415%,1.790%。结论该方法简便准确,专属性强,重复性好,可用于蒙药阿那日-4的质量控制方法。
Objective To establish an HPLC method for the simultaneous determination of ellagic acid,cinnamaldehyde and piperine in Anari-4. Methods The chromatographic separation was carried out on an Agilent ZORBAX Eclipse XDB-C18 column(250 mm×4.6 mm, 5 μm) with the mobile phase consisting of methanol-0.1 %(v/v) phosphoric acid solution in a gradient mode at the flow rate of 1.0 ml/min. The detection wavelength was set at 271 nm and the column temperature was 30 ℃. Results The sample solution was stable within 24 h, the method showed good linear relationship within the range of 0.050-0.500 mg/ml for ellagic acid(r=0.9994), 0.030-0.300 mg/ml for cinnamaldehyde(r=0.9995) and 0.030-0.300 mg/ml for piperine(r=0.9997); the average recoveries were 101.68 %,100.94 % and 98.55 %, respectively; and the RSDs were 1.806 %, 1.415 % and 1.790 %, respectively. Conclusion The method is handy and accurate, with strong speci?city, and could be used as the quality control of mongolian medicine Anari-4.
Objective To establish an HPLC method for the simultaneous determination of ellagic acid,cinnamaldehyde and piperine in Anari-4. Methods The chromatographic separation was carried out on an Agilent ZORBAX Eclipse XDB-C18 column(250 mm×4.6 mm, 5 μm) with the mobile phase consisting of methanol-0.1 %(v/v) phosphoric acid solution in a gradient mode at the flow rate of 1.0 ml/min. The detection wavelength was set at 271 nm and the column temperature was 30 ℃. Results The sample solution was stable within 24 h, the method showed good linear relationship within the range of 0.050-0.500 mg/ml for ellagic acid(r=0.9994), 0.030-0.300 mg/ml for cinnamaldehyde(r=0.9995) and 0.030-0.300 mg/ml for piperine(r=0.9997); the average recoveries were 101.68 %,100.94 % and 98.55 %, respectively; and the RSDs were 1.806 %, 1.415 % and 1.790 %, respectively. Conclusion The method is handy and accurate, with strong speci?city, and could be used as the quality control of mongolian medicine Anari-4.
引文
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[12]玉华.HPLC法测定蒙药阿那日十四味散中胡椒碱的含量[J].中国民族医药杂志,2013,19(4):55-56.
[13]Merran H,Muzammil A,Sauel.W C,et al.Pomegranate(Punica granatum)purified polyphenol extract inhibits influence virus and has a synergistic effect with oseltamivir[J].Phytomedicine,2009,16(12):1127-1126.
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[15]Morimoto S,Nonaka G,Nishioka I.Tannins and related compounds.XXXV.Proanthocyanidins with a doubly linked unit from the rootbark of Cinnamomum sieboldii Meisner[J].Chem Pharm Bull,1985,33(10):4338-4345.
[16]Killday K B,Davey M H,Glinski J A,et al.Bioactive A-type proanthocyanidins from Cinnamomum cassia[J].Nat Prod,2011,74(9):1833-18413.
[17]张海涛,吕颖.高效液相色谱法测定蒙药制剂敖勒盖-13中胡椒碱的含量[J].中国民族医药杂志,2017,23(5):42-44.
[18]卢婷,高鸿亮,席利力,等.胡椒碱对胆汁反流性胃炎大鼠胃粘膜的作用研究[J].中药药理与临床,2014,(05):26-29.
[2]王欢,王秀兰,吴龙堂.蒙药阿那日-4散研究进展[J].中国中医药信息杂志,2009,16(S1):86-87.
[3]王欢,王秀兰吴龙堂.蒙成药阿那日-4对小鼠乙醇及应激反应引起胃溃疡的影响[J].中国现代应用药学,2011,28(03):201-204.
[4]王欢,佟山.蒙成药阿那日-4对大鼠幽门结扎性胃溃疡的影响[J].中国民族医药杂志,2011,17(1):40-42.
[5]温永顺.浅析蒙药阿那日-4配伍特点[J].世界最新医学信息文摘,2017,(9):189-191.
[6]杨宏昕,白音夫,党小菊.阿那日五味散对实验动物胃肠功能影响的研究[J].内蒙古医学杂志,2011,33(3):210-212.
[7]李梅,布日额.蒙医经典方“阿那日-8”的整理研究[J].北方药学,2010,7(2):36-40.
[8]齐双山,萨茹拉,席月亮.阿那日-14对萎缩性胃炎伴异型增生的逆转治疗研究[J].中国民族医药杂志,2006,12(1):5.
[9]张明玉,哈斯巴根.慢性萎缩性胃炎应用蒙药治疗的疗效观察[J].临床医药文献电子杂志,2007,4(10):1935-1937.
[10]巴根那,吴香杰,包桂花,等.HPLC法测定蒙药阿那日五味散中胡椒碱的含量[J].辽宁中医杂志,2008,35(1):111-112.
[11]布日额,李梅.蒙药“阿那日-8”中金属元素含量测定[J].中成药,2010,32(11):2025-2026.
[12]玉华.HPLC法测定蒙药阿那日十四味散中胡椒碱的含量[J].中国民族医药杂志,2013,19(4):55-56.
[13]Merran H,Muzammil A,Sauel.W C,et al.Pomegranate(Punica granatum)purified polyphenol extract inhibits influence virus and has a synergistic effect with oseltamivir[J].Phytomedicine,2009,16(12):1127-1126.
[14]Cam M,Hisil Y.Pressurised water extraction of polyphenols from pomegranate peels[J].Food Chem,2010,123(3):878-885.
[15]Morimoto S,Nonaka G,Nishioka I.Tannins and related compounds.XXXV.Proanthocyanidins with a doubly linked unit from the rootbark of Cinnamomum sieboldii Meisner[J].Chem Pharm Bull,1985,33(10):4338-4345.
[16]Killday K B,Davey M H,Glinski J A,et al.Bioactive A-type proanthocyanidins from Cinnamomum cassia[J].Nat Prod,2011,74(9):1833-18413.
[17]张海涛,吕颖.高效液相色谱法测定蒙药制剂敖勒盖-13中胡椒碱的含量[J].中国民族医药杂志,2017,23(5):42-44.
[18]卢婷,高鸿亮,席利力,等.胡椒碱对胆汁反流性胃炎大鼠胃粘膜的作用研究[J].中药药理与临床,2014,(05):26-29.