HPLC波长切换法同时测定苍柏祛痛胶囊中的6种成分
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摘要
目的采用HPLC波长切换法同时测定苍柏祛痛胶囊中盐酸黄柏碱、龙胆苦苷、芍药苷、粉防己碱、盐酸小檗碱、丹皮酚等6种成分的含量。方法色谱柱为Waters Xselect CSH-C_(18)柱(150 mm×4. 6 mm,5μm),流动相为乙腈-0. 1%磷酸溶液,梯度洗脱(0～15 min、10%→18%A,15～30 min、18%→50%A,30～35 min、50%→10%A),流速为1. 0 m L·min~(-1),波长为284 nm(0～7 min,盐酸黄柏碱)、274 nm(7～10 min,龙胆苦苷)、230 nm(10～14 min,芍药苷)、274 nm(14～35 min,粉防己碱、盐酸小檗碱、丹皮酚),进样量10μL。结果盐酸黄柏碱、龙胆苦苷、芍药苷、粉防己碱、盐酸小檗碱、丹皮酚进样量分别在0. 150～1. 504、0. 768～7. 680、1. 096～10. 960、0. 220～2. 200、0. 296～2. 956、0. 0345～0. 345μg时与峰面积呈良好的线性关系;平均回收率分别为98. 3%、99. 2%、98. 8%、98. 8%、99. 1%、98. 2%,RSD分别为1. 32%、1. 46%、1. 08%、1. 31%、1. 26%、1. 21%(n=6)。结论所用方法可同时测定制剂中的多种成分,操作简便、准确,重复性好,可用于复方苍柏祛痛胶囊的质量控制。
OBJECTIVE To develop an HPLC wavelength switching method for the simultaneous determination content of 6 components in Cangbai qutong capsules. METHODS The quantitative analysis was carried out on a column of Waters Xselect CSH-C_(18)( 150 mm × 4. 6 mm,5 μm),using a mobile phase of acetonitrile( A)-0. 1% phosphoric acid( B) by a gradient eluted program( 0-15 min,10% →18% A; 15-30 min,18 % →50% A; 30-35 min,50 % →10% A) with a flow rate of 1 m L·min~(-1) at 30 ℃. The detection wavelength was set at 284 nm( 0-7 min,phellodendrine chloride),274 nm( 7-10 min,gentiopicroside),230 nm( 10-14 min,paeoniflorin),and 274 nm( 14-35 min,tetrandrine,berberine hydrochloride,paeonol).RESULTS The linear ranges of phellodendrine chloride,gentiopicroside,paeoniflorin,tetrandrine,berberine hydrochloride and paeonol fell within the ranges of 0. 150-1. 504 μg,0. 768-7. 680 μg,1. 096-10. 960 μg,0. 220-2. 200 μg,0. 296-2. 956μg and 0. 0345-0. 345 μg,respectively. The recoveries of phellodendrine chloride,gentiopicroside,paeoniflorin,tetrandrine,berberine hydrochloride and paeonol were 98. 3%,99. 2%,98. 8%,98. 8%,99. 1% and 98. 2%,respectively,and the relative standard deviations were 1. 32%,1. 46%,1. 08%,1. 31%,1. 26% and 1. 21%( n = 6),respectively. CONCLUSION This method is simple,accurate,reproducible and convenient for the quality control of Cangbai qutong capsules.
OBJECTIVE To develop an HPLC wavelength switching method for the simultaneous determination content of 6 components in Cangbai qutong capsules. METHODS The quantitative analysis was carried out on a column of Waters Xselect CSH-C_(18)( 150 mm × 4. 6 mm,5 μm),using a mobile phase of acetonitrile( A)-0. 1% phosphoric acid( B) by a gradient eluted program( 0-15 min,10% →18% A; 15-30 min,18 % →50% A; 30-35 min,50 % →10% A) with a flow rate of 1 m L·min~(-1) at 30 ℃. The detection wavelength was set at 284 nm( 0-7 min,phellodendrine chloride),274 nm( 7-10 min,gentiopicroside),230 nm( 10-14 min,paeoniflorin),and 274 nm( 14-35 min,tetrandrine,berberine hydrochloride,paeonol).RESULTS The linear ranges of phellodendrine chloride,gentiopicroside,paeoniflorin,tetrandrine,berberine hydrochloride and paeonol fell within the ranges of 0. 150-1. 504 μg,0. 768-7. 680 μg,1. 096-10. 960 μg,0. 220-2. 200 μg,0. 296-2. 956μg and 0. 0345-0. 345 μg,respectively. The recoveries of phellodendrine chloride,gentiopicroside,paeoniflorin,tetrandrine,berberine hydrochloride and paeonol were 98. 3%,99. 2%,98. 8%,98. 8%,99. 1% and 98. 2%,respectively,and the relative standard deviations were 1. 32%,1. 46%,1. 08%,1. 31%,1. 26% and 1. 21%( n = 6),respectively. CONCLUSION This method is simple,accurate,reproducible and convenient for the quality control of Cangbai qutong capsules.
引文
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[11]胡云飞,徐国兵.牡丹皮及其主要成分丹皮酚的药理作用研究进展[J].安徽医药,2014,18(4):589-592.
[2]肖礼娥,余佳文,原欢欢,等. HPLC法同时测定通天口服液中的3种有效成分[J].华西药学杂志,2016,31(5):524-526.
[3]林林,林永强,汪冰,等. HPLC波长切换技术同时测定复方黄连素片中6个成分的含量[J].药物分析杂志,2014,34(1):96-99.
[4]孙冰婷,居文政,戴国梁,等. HPLC同时测定白香丹胶囊中的3种成分[J].华西药学杂志,2016,31(3):295-297.
[5]尤春雪,张振秋,李峰,等. HPLC波长切换技术对葛根中8种成分的测定及指纹图谱研究[J].中草药,2013,44(5):616-621.
[6]彭爱华,杨宏,杨林,等. RP-HPLC测定不同采收期川黄柏中小檗碱、黄柏碱的含量[J].华西药学杂志,2006,21(4):377-379.
[7]廉莲,贾天柱.黄柏及其炮制品的抗痛风作用研究[J].安徽农业科学,2011,39(15):8911-8912,8932.
[8]年蔚.麻花秦艽醇提物对大鼠急性胃黏膜损伤的保护作用研究[D].西宁:青海大学硕士学位论文,2011.
[9]陆小华,马骁,王建,等.赤芍的化学成分和药理作用研究进展[J].中草药,2015,46(4):595-602.
[10]刑志博,王凤梅,王翠平,等.粉防己有效成分的药理活性研究进展[J].中国实验方剂学杂志,2014,20(9):241-246.
[11]胡云飞,徐国兵.牡丹皮及其主要成分丹皮酚的药理作用研究进展[J].安徽医药,2014,18(4):589-592.