摘要
[目的]从分子水平分析昭通牛的遗传多样性、群体遗传结构及父系起源。[方法]利用PCR产物直接测序和荧光微卫星分型方法对24头昭通牛2个Y-SNPs标记(UTY-19和ZFY-10)和2个Y-STRs标记(INRA189和BM861)进行遗传多态性检测。[结果]发现昭通牛有Y2-90-158和Y3-88-156两种单倍型(Y-SNPs-INRA189-BM861),频率分别为29.17%和70.83%,表明昭通牛有普通牛和瘤牛两种父系起源。Y染色体单倍型多样度为0.4312±0.0812,说明昭通牛的遗传多样性较高。[结论]昭通牛以瘤牛Y3单倍型组为主,具有南方黄牛特征,与其地理分布和气候及形态特征相一致。
[Objective] To explore the paternal genetic diversity, population genetic structure and origin of Zhaotong cattle at the molecular level. [Method] Direct sequencing and fluorescence microsatellite typing were used. [Result] The genetic diversity of 24 Zhaotong cattle samples were analyzed using two Y-SNPs markers(UTY-19 and ZFY-10) and two Y-STRs markers(IRNA189 and BM861). The results showed that there were two Y-chromosome haplotypes(Y2-90-158 and Y3-88-156) in Zhaotong cattle, their frequencies were 29.17% and 70.83%, respectively, indicating that Zhaotong cattle breed belongs to two paternal origins of Bos taurus and Bos indicus. The haplotype diversity of Y chromosome was 0.4312±0.0812, indicating high genetic diversity of Zhaotong cattle. [Conclusion] Zhaotong cattle breed is mainly dominated by zebu Y3 haplogroup and has the characteristics of Chinese southern cattle group, which is consistent with its geographical distribution, climatic and morphological characteristics.
引文
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