豌豆肽对环磷酰胺致免疫抑制小鼠免疫功能的影响
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摘要
研究豌豆肽对免疫抑制小鼠的免疫调节作用。腹腔注射环磷酰胺(cyclophosphamide,CTX)建立小鼠免疫抑制模型,并设置空白对照组,观察不同剂量豌豆肽[低剂量组0.4 g/(kg·bw)、中剂量组0.8 g/(kg·bw)、高剂量组1.6 g/(kg·bw)]干预15 d后对小鼠体重、免疫脏器重量及形态、白细胞计数、脾脏T淋巴细胞亚群、免疫球蛋白含量、细胞因子水平、骨髓有核细胞计数和DNA含量的影响。模型组小鼠脾脏和胸腺镜下形态学结构紊乱,外周血白细胞计数、CD3~+、CD4~+、CD8~+T淋巴细胞百分比、免疫球蛋白Ig G和Ig M含量、骨髓有核细胞浓度和DNA含量均显著低于对照组(p<0.01),细胞因子IFN-γ浓度明显低于对照组(p<0.05)。与模型组相比,豌豆肽组小鼠在以上指标有不同程度的改善。一定剂量的豌豆肽对CTX致免疫抑制小鼠的免疫功能具有一定的改善作用。
The aim of this study was to investigate the immuno-regulatory effects of pea peptides on immunosuppressive mice. Mice injected with cyclophosphamide( CTX) was the model group and normal saline was the control group. Different dosages of pea peptide(0. 4 g/( kg·bw),0. 8 g/( kg·bw),1. 6 g/( kg·bw) were given to low,middle and high groups for 15 days. The body weight,spleen,and thymus weight of mice,morphologic variations of immune organs,white blood cell count,T lymphocyte subsets of spleen,immunoglobulin content,cytokine levels,bone marrow nucleated cell count and DNA content were tested. The morphological changes of spleen and thymus were observed under microscope in the model group. The number of white blood cell,proportion of CD~(3 +),CD~(4 +),CD~(8 +)T lymphocyte,concentration of immunoglobulin Ig G and Ig M,and nucleated cell counts and DNA density in bone marrow were significantly lower than the control group( p < 0. 01). The concentration of IFN-γ was significantly lower than that of the control group( p < 0. 05). Compared with the model group,pea peptide groups showed different degrees of improvement in the above indicators. Pea peptide can improve the immune function of immunosuppressive mice induced by CTX.
The aim of this study was to investigate the immuno-regulatory effects of pea peptides on immunosuppressive mice. Mice injected with cyclophosphamide( CTX) was the model group and normal saline was the control group. Different dosages of pea peptide(0. 4 g/( kg·bw),0. 8 g/( kg·bw),1. 6 g/( kg·bw) were given to low,middle and high groups for 15 days. The body weight,spleen,and thymus weight of mice,morphologic variations of immune organs,white blood cell count,T lymphocyte subsets of spleen,immunoglobulin content,cytokine levels,bone marrow nucleated cell count and DNA content were tested. The morphological changes of spleen and thymus were observed under microscope in the model group. The number of white blood cell,proportion of CD~(3 +),CD~(4 +),CD~(8 +)T lymphocyte,concentration of immunoglobulin Ig G and Ig M,and nucleated cell counts and DNA density in bone marrow were significantly lower than the control group( p < 0. 01). The concentration of IFN-γ was significantly lower than that of the control group( p < 0. 05). Compared with the model group,pea peptide groups showed different degrees of improvement in the above indicators. Pea peptide can improve the immune function of immunosuppressive mice induced by CTX.
引文
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[18]彭小彬,邱小惠,余传林,等.茯苓多糖对环磷酰胺所致免疫功能低下小鼠体液免疫功能的影响[J].中药药理与临床,2013,29(5):69-72.
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[20]吴婷,陈广洁.miRNA调控Th细胞分化的研究进展[J].现代免疫学,2015,35(4):334-337.
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[22]淮文英,杨福权,唐玉琴,等.表观遗传学调控Th细胞分化发育的研究进展[J].生物学杂志,2016,33(4):70-72.
[2]WALTHER B,SIEBER R.Bioactive proteins and peptides in foods[J].International Journal for Vitamin and Nutrition Research,2011,81(2/3):181-192.
[3]刘铭,刘玉环,王允圃,等.制备、纯化和鉴定生物活性肽的研究进展及应用[J].食品与发酵工业,2016,42(4):244-251.
[4]樊金娟,张幼竹.植物源低聚肽的研究进展[J].食品科技,2009,34(7):175-178.
[5]郭兴凤,莫重文.豌豆蛋白粉的制取研究[J].郑州粮食学院学报,1995(3):70-73.
[6]刁静静,于伟,张丽萍.豌豆蛋白水解物的分离及其抗氧化活性的研究[J].包装与食品机械,2013,31(3):25-29.
[7]许丹,林峰,朱小语,等.牡蛎肽对免疫抑制小鼠免疫功能的影响[J].北京大学学报(医学版),2016,48(3):392-397.
[8]季屹红,倪美鑫,蔡晶,等.补康灵对辐射损伤小鼠造血功能的影响[J].中国实验方剂学杂志,2012,18(7):184-186.
[9]赵岩,张楚辞,郑国良,等.添加丙氨酰谷氨酰胺的胃肠外营养对存在营养风险的胃癌根治术患者免疫功能、营养状态及术后恢复的临床意义[J].现代生物医学进展,2012,12(19):3 693-3 697.
[10]杨屹立,郭天康,詹渭鹏,等.谷氨酰胺强化肠内营养对胃癌术后免疫功能影响的Meta分析[J].现代肿瘤医学,2015,23(5):658-662.
[11]刘婷婷,张帅,邓斐月,等.谷氨酰胺与丁酸梭菌对断奶仔猪生长性能、免疫功能、小肠形态和肠道菌群的影响[J].动物营养学报,2011,23(6):998-1 005.
[12]刘巧婷,何若钢,刘谨,等.谷氨酰胺对保育猪生长性能、免疫器官及其小肠形态学发育程度影响的研究[J].饲料工业,2014,35(15):35-40.
[13]钟金凤,方热军.环磷酰胺免疫抑制机制及在动物模型上的应用[J].中国免疫学杂志,2016,32(10):1 541-1 546.
[14]HALEY P,PERRY R,ENNULAT D,et al.STP position paper:best practice guideline for the routine pathology e-valuation of the immune system[J].Toxicologic Pathology,2005,33(3):404-407.
[15]朱小语,许丹,陈晓文,等.乌鸡肽对环磷酰胺致免疫低下小鼠的免疫调节作用[J].食品与发酵工业,2016,42(4):44-49.
[16]张俊,黄睿,刘勇,等.云芝糖肽对环磷酰胺所致免疫抑制小鼠免疫保护作用[J].辽宁中医药大学学报,2013,15(7):47-50.
[17]WANG M Z,DING L Y,GAO J,et al.Effects of dietary n-6/n-3 polyunsaturated fatty acid ratios on the mass,and histological and ultrastructures of liver,spleen and thymus of 70-day-old Yangzhou goslings[J].Journal of Animal Physiology and Animal Nutrition,2016,100(2):391-400.
[18]彭小彬,邱小惠,余传林,等.茯苓多糖对环磷酰胺所致免疫功能低下小鼠体液免疫功能的影响[J].中药药理与临床,2013,29(5):69-72.
[19]FUENTES-ARDERIU X,MESTRE M.Description of flow cytometry examinations related to human cell differentiation molecules in clinical immunology[J].Cytometry Part B Clinical Cytometry,2010,76B(4):291-293.
[20]吴婷,陈广洁.miRNA调控Th细胞分化的研究进展[J].现代免疫学,2015,35(4):334-337.
[21]路敏敏,金世禄.Th9/IL-9与炎性疾病研究进展[J].中华临床医师杂志(电子版),2015,9(2):300-303.
[22]淮文英,杨福权,唐玉琴,等.表观遗传学调控Th细胞分化发育的研究进展[J].生物学杂志,2016,33(4):70-72.