摘要
拮抗真菌是乳酸菌的重要生物学特性之一。本研究采用双层平板法对12株植物乳杆菌抗真菌特性进行了初步评价,并对其无菌上清液在不同温度和蛋白酶处理条件下的抑菌效果进行了分析。结果表明,IMAU80095对不同指示真菌均有较好的抑菌效果;经过胰蛋白酶处理的无菌上清液对黑曲霉无抑制作用,对其他5株真菌有轻微抑制作用;热处理和pH的变化对无菌上清液的抑菌活性有一定影响。这些研究结果,可为抗真菌活性乳酸菌发酵剂的研究开发提供理论依据。
Antifungal property is one of the important biological characteristics of Lactic acid bacteria(LAB). In this study, the antifungal properties of 12 Lactobacillus plantarum strains were evaluated using the double-layer plate method, and the antifungal effects of their aseptic supernatants under different temperatures and protease treatment conditions were analyzed. The results showed that IMAU80095 had good antifungal effect on different indicator fungi; the aseptic supernatant treated with trypsin had no inhibitory effect on Aspergillus niger and slight inhibition on other 5 strains of fungi; Heat treatment and pH changes have a certain influence on the antifungal activity of the sterile supernatant.These research results can provide a theoretical basis for the research and development of LAB starter with antifungal activity.
引文
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