低温驯化对南方小花蝽冷藏的影响
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摘要
为探讨低温驯化对南方小花蝽雌成虫冷藏的影响,在室内15℃下饲养7d作为低温驯化处理,研究了驯化后与未驯化的南方小花蝽雌成虫分别在4、6和10℃下冷藏其寿命、存活率和致死中时间(LT50)的异同。结果表明,无论是否进行低温驯化,南方小花蝽雌成虫的寿命都显著大于对照(25℃下饲养)。在10℃冷藏条件下,是否经过低温驯化对南方小花蝽雌成虫寿命没有明显影响,但在4℃和6℃条件下,低温驯化能明显延长雌成虫的寿命,分别延长了80.7%和83.7%。南方小花蝽雌成虫存活率均随冷藏时间的延长而下降,在同一低温冷藏温度下经低温驯化处理的南方小花蝽存活率比未驯化的高。经低温驯化的南方小花蝽雌成虫在4、6和10℃冷藏时,其LT50分别比未经驯化的延长了30.0%、57.8%和40.4%。以上结果表明南方小花蝽的寿命具有可塑性,低温驯化有利于南方小花蝽的冷藏,6℃可作为南方小花蝽长期储藏的温度。
To evaluate the effect of cold acclimation on cold storage of Orius similis, the longevity, survival rate and lethal time(Lt50) of female adults of O. similis were measured for the insects treated at 4 ℃, 6 ℃ and 10 ℃, with or without previous cold acclimation for 7 days at 15 ℃. The cold-stored O. similis female adults lived significantly longer than the control adults reared at 25 ℃, irrespective of cold acclimation. Cold acclimation had no effect on the longevity of the female adults stored at 10 ℃, but extended the longevity of the adults stored at 4 ℃ and 6 ℃ by 80.7% and 83.7%, respectively. The survival rate of the female adults decreased with extension of cold storage time, but the acclimated O. similis had relatively high survival rate compared to those without acclimation. In cold acclimated female adults stored at 4 ℃, 6 ℃ and 10 ℃, the LT50 increased by 30.0%, 57.8% and 40.4%, respectively, in comparison with those without acclimation. The result indicates that the longevity of O. similis female adults is responsive to cold acclimation. Cold acclimation is benefical to the cold storage of O. similis, especially at 6 ℃.
To evaluate the effect of cold acclimation on cold storage of Orius similis, the longevity, survival rate and lethal time(Lt50) of female adults of O. similis were measured for the insects treated at 4 ℃, 6 ℃ and 10 ℃, with or without previous cold acclimation for 7 days at 15 ℃. The cold-stored O. similis female adults lived significantly longer than the control adults reared at 25 ℃, irrespective of cold acclimation. Cold acclimation had no effect on the longevity of the female adults stored at 10 ℃, but extended the longevity of the adults stored at 4 ℃ and 6 ℃ by 80.7% and 83.7%, respectively. The survival rate of the female adults decreased with extension of cold storage time, but the acclimated O. similis had relatively high survival rate compared to those without acclimation. In cold acclimated female adults stored at 4 ℃, 6 ℃ and 10 ℃, the LT50 increased by 30.0%, 57.8% and 40.4%, respectively, in comparison with those without acclimation. The result indicates that the longevity of O. similis female adults is responsive to cold acclimation. Cold acclimation is benefical to the cold storage of O. similis, especially at 6 ℃.
引文
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[22]赵静,陈珍珍,曲建军,等.异色瓢虫成虫冷驯化反应及体内几种酶活力的相关变化[J].昆虫学报,2010,53(2):147-153.
[2]王方海,周伟儒,王韧.东亚小花蝽的生物学及其人工繁殖[J].昆虫天敌,1998,20(1):43-45.
[3]张士昶,周兴苗,潘悦,等.南方小花蝽液体人工饲料的饲养效果评价[J].昆虫学报,2008,51(9):997-1001.
[4]魏潮生,彭中健,杨广球,等.南方小花蝽的初步研究[J].昆虫天敌,1984,6(1):32-40.
[5]孙晓会,徐学农,王恩东.东亚小花蝽对西方花蓟马和二斑叶螨的捕食选择性[J].生态学报,2009,29(11):6285-6291.
[6]吕兵,孙猛,翟一凡,等.短期食物驯化对麦蛾卵饲养的东亚小花蝽的捕食功能反应影响[J].环境昆虫学报,2018,40(1):64-69.
[7]曾广,郅军锐,张昌容,等.南方小花蝽对烟蚜的捕食作用[J].西南师范大学学报(自然科学版),2018,43(1):82-88.
[8]Nagayi K.Rearing method of native enemies in Japan:rearing technique of Orius sauteri[J].Japan Plant Protection,1997,51:519-522.
[9]杨丽文,王甦,张志勇,等.米蛾卵饲养东亚小花蝽的关键点研究[J].环境昆虫学报,2014,36(6):971-977.
[10]谭晓玲,王甦,李修炼,等.东亚小花蝽人工饲料微胶囊剂型的研制及饲养效果评价[J].昆虫学报,2010,53(8):891-900.
[11]张瑜红.南方小花蝽人工扩繁饲养的研究[D].武汉:华中农业大学,2015.
[12]张士昶.南方小花蝽人工规模化饲养技术的研究[D].武汉:华中农业大学,2009.
[13]张烨,连梅力,李唐,等.不同低温驯化条件对玉米螟赤眼蜂低温贮藏的影响[J].中国生物防治学报,2016,32(2):277-281.
[14]Lessard E,Boivin G.Effect of low temperature on emergence,fecundity,longevity and host-feeding by Trichogramma brassicae[J].BioControl,2013,58(3):319-329.
[15]赵静.异色瓢虫低温胁迫耐受性及其越冬策略的研究[D].泰安:山东农业大学,2011.
[16]郭建英,吴岷,万方浩.东亚小花蝽成虫和卵的耐冷藏性[J].中国生物防治学报,2002,18(1):10-12.
[17]曾广,郅军锐,张昌容.南方小花蝽控制害虫的研究现状[J].山地农业生物学报,2017,36(1):71-75.
[18]周兴苗.南方小花蝽大量繁殖关键技术及其田间释放生态学基础研究[D].武汉:华中农业大学,2008.
[19]岳雷,郭建英,周忠实,等.冷驯化对昆虫耐寒性及其适合度的影响[J].中国生物防治学报,2013,29(2):286-293.
[20]Hoffmann A A,S?rensen J G,Loeschcke V.Adaptation of Drosophila to temperature extremes:bringing together quantitative and molecular approaches[J].Journal of Thermal Biology,2003,28(3):175-216.
[21]曹凯丽,王芳艳,胡敏,等.驯化对始红蝽(Pyrrhocoris apterus)耐寒能力的影响及越冬适应策略[J].生态学报,2018,38(5):1826-1831.
[22]赵静,陈珍珍,曲建军,等.异色瓢虫成虫冷驯化反应及体内几种酶活力的相关变化[J].昆虫学报,2010,53(2):147-153.