LncRNA H19、miR-29b、miR-124在子宫内膜癌组织中的表达及临床意义
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摘要
目的探讨子宫内膜癌组织中长链非编码RNA H19(LncRNA H19)、微小RNA-29b(miR-29b)、微小RNA-124(miR-124)表达水平,以及其与子宫内膜癌临床特征及预后的关系。方法选取2012年9月至2015年9月湖北省荆州市第三人民医院收治的90例行子宫切除术的子宫内膜癌患者为受试对象,术中留取子宫内膜癌组织和相应癌旁组织,另选取同期因子宫肌瘤切除子宫的85例正常子宫内膜组织(经病理检查证实)。采用实时荧光定量聚合酶链反应(qRT-PCR)检测组织中LncRNA H19、miR-29b、miR-124表达情况,分析它们的表达水平与子宫内膜癌患者临床特征的关系,并观察3年总生存率(OS)。结果子宫内膜癌组织LncRNA H19相对表达量显著高于癌旁组织和正常子宫内膜组织(P<0.05),miR-29b、miR-124相对表达量明显低于癌旁组织和正常子宫内膜组织(P<0.05)。LncRNA H19、miR-29b、miR-124表达水平与子宫内膜癌患者国际妇产科联合会(FIGO)分期、病理分级、子宫肌层浸润深度及盆腔淋巴结转移有关(P<0.05)。子宫内膜癌患者LncRNA H19表达水平与miR-29b、miR-124均呈负相关(P<0.05)。LncRNA H19低表达患者3年OS显著高于高表达患者(P<0.05),miR-29b、miR-124高表达患者3年OS明显高于低表达患者(P<0.05)。FIGO分期、子宫肌层浸润深度,以及LncRNA H19、miR-29b、miR-124表达水平是影响子宫内膜癌患者预后的独立危险因素。结论子宫内膜癌组织中LncRNA H19高表达,miR-29b、miR-124低表达。
Objective To investigate the expressions of long non-coding RNA H19(LncRNA H19),micro RNA 29b(miR-29b)and micro RNA 124(miR-124)in endometrial carcinoma tissues and their relationship with clinical features and prognosis of endometrial cancer.Methods Ninety patients with endometrial cancer treated by hysterectomy in the Jingzhou Municipal Third People′s Hospital from September 2012 to September 2015 were selected as the subjects.The endometrial cancer tissues and corresponding paracancerous tissues were preserved during operation,other contemporaneous 85 cases of normal endometrium tissue due to hysteromyoma resection(confirmed by pathological examination)were selected.The qRT-PCR method was adopted to detect the expressions of LncRNA H19,miR-29b and miR-124 in tissues,their expressions in different tissues and their relationships with the clinical characteristics in the patients with endometrial cancer were analyzed,moreover the 3-year overall survival rate(OS)was observed.Results The relative expression level of LncRNA H19 in endometrial carcinoma tissues was significantly higher than that in paracancerous tissues and normal endometrial tissues(P<0.05),the relative expression levels of miR-29 band miR-124 were significantly lower than those in paracancerous tissues and normal endometrium tissues(P<0.05).The expression levels of LncRNA H19,miR-29 band miR-124 were related with FIGO stage,pathological grade,depth of myometrial invasion and pelvic lymph node metastasis(P<0.05).The expression level of LncRNA H19 was negatively correlated with miR-29b and miR-124 expressions in the patients with endometrial carcinoma(P<0.05).The 3-year OS in the patients with low expression of LncRNA H19 was significantly higher than that in the patients with high expression(P<0.05),the 3-year OS in the patients with high expressions of miR-29b and miR-124 was significantly higher than those in the patients with low expressions(P<0.05).FIGO staging,myometrial invasion depth,LncRNA H19,miR-29b and miR-124 expressions were independent risk factors affecting the prognosis in the patients with endometrial cancer.Conclusion LncRNA H19 in endometrial carcinoma tissue is highly expressed,while miR-29b and miR-124 are lowly expressed.
Objective To investigate the expressions of long non-coding RNA H19(LncRNA H19),micro RNA 29b(miR-29b)and micro RNA 124(miR-124)in endometrial carcinoma tissues and their relationship with clinical features and prognosis of endometrial cancer.Methods Ninety patients with endometrial cancer treated by hysterectomy in the Jingzhou Municipal Third People′s Hospital from September 2012 to September 2015 were selected as the subjects.The endometrial cancer tissues and corresponding paracancerous tissues were preserved during operation,other contemporaneous 85 cases of normal endometrium tissue due to hysteromyoma resection(confirmed by pathological examination)were selected.The qRT-PCR method was adopted to detect the expressions of LncRNA H19,miR-29b and miR-124 in tissues,their expressions in different tissues and their relationships with the clinical characteristics in the patients with endometrial cancer were analyzed,moreover the 3-year overall survival rate(OS)was observed.Results The relative expression level of LncRNA H19 in endometrial carcinoma tissues was significantly higher than that in paracancerous tissues and normal endometrial tissues(P<0.05),the relative expression levels of miR-29 band miR-124 were significantly lower than those in paracancerous tissues and normal endometrium tissues(P<0.05).The expression levels of LncRNA H19,miR-29 band miR-124 were related with FIGO stage,pathological grade,depth of myometrial invasion and pelvic lymph node metastasis(P<0.05).The expression level of LncRNA H19 was negatively correlated with miR-29b and miR-124 expressions in the patients with endometrial carcinoma(P<0.05).The 3-year OS in the patients with low expression of LncRNA H19 was significantly higher than that in the patients with high expression(P<0.05),the 3-year OS in the patients with high expressions of miR-29b and miR-124 was significantly higher than those in the patients with low expressions(P<0.05).FIGO staging,myometrial invasion depth,LncRNA H19,miR-29b and miR-124 expressions were independent risk factors affecting the prognosis in the patients with endometrial cancer.Conclusion LncRNA H19 in endometrial carcinoma tissue is highly expressed,while miR-29b and miR-124 are lowly expressed.
引文
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[12]吴晓燕,王芳,孙倩,等.宫颈癌组织中miR-124、miR-124-5p表达变化及意义[J].山东医药,2018,58(19):48-50.
[13]吕细林,黄刚,肖曼,等.过表达长链非编码RNAH19促进miR-124-3p表达抑制肝癌细胞增殖[J].第三军医大学学报,2016,38(6):554-559.
[2]SUBRAMANIAN M,RAO S R,THACKER P,et al.MiR-29bdownregulates canonical wnt signaling by suppressing coactivators ofβ-catenin in human colorectal cancer cells[J].J Cell Biochem,2015,115(11):1974-1984.
[3]陈鸣,刘建华.磁共振成像与术中病灶探查诊断子宫内膜癌肌层浸润及盆腹腔淋巴结转移临床价值研究[J].中国实用妇科与产科杂志,2016,32(2):159-162.
[4]HUA Q,LV X,GU X,et al.Genetic variants in lncRNAH19are associated with the risk of bladder cancer in a Chinese population[J].Mutagenesis,2016,31(5):531-538.
[5]YUAN L,ZHOU C,LU Y,et al.IFN-γ-mediated IRF1/miR-29bfeedback loop suppresses colorectal cancer cell growth and metastasis by repressing IGF1[J].Cancer Lett,2015,359(1):136-147.
[6]ZHANG T H,LIANG L Z,LIU X L,et al.Long non-coding RNA MALAT1interacts with miR-124and modulates tongue cancer growth by targeting JAG1[J].Oncol Rep,2017,37(4):2087-2094.
[7]SU Z,ZHI X,ZHANG Q,et al.LncRNA H19functions as a competing endogenous RNA to regulate AQP3expression by sponging miR-874in the intestinal barrier[J].Febs Lett,2016,590(9):1354-1364.
[8]孙士成,赵虎,刘锐,等.长链非编码RNA H19在前列腺癌中的表达及对前列腺癌细胞糖代谢与生长的影响[J].中华男科学杂志,2017,23(2):120-124.
[9]DI J,DU J,ZHANG X,et al.miR-124promotes the neuronal differentiation of mouse inner ear neural stem cells[J].Int J Mol Med,2016,38(5):1367-1376.
[10]马肖,袁伟,唐万燕,等.miR-29b在结直肠癌组织中的表达及其临床意义[J].中华肿瘤杂志,2016,38(6):430-433.
[11]刘锋,谢黎明,张志伟,等.miR-124在胃癌中的表达及临床意义[J].中国癌症杂志,2016,26(3):215-220.
[12]吴晓燕,王芳,孙倩,等.宫颈癌组织中miR-124、miR-124-5p表达变化及意义[J].山东医药,2018,58(19):48-50.
[13]吕细林,黄刚,肖曼,等.过表达长链非编码RNAH19促进miR-124-3p表达抑制肝癌细胞增殖[J].第三军医大学学报,2016,38(6):554-559.