半乳糖凝集素-1、p16蛋白表达与宫颈癌患者临床特征的关系
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摘要
目的探讨宫颈癌组织中半乳糖凝集素-1(Gal-1)、p16蛋白的表达情况及与宫颈癌患者临床特征的关系。方法选取100例宫颈癌患者的宫颈癌组织标本和100例宫颈上皮瘤变(CIN)患者的CIN组织标本。采用免疫组织化学染色法检测宫颈癌组织、CIN组织中Gal-1、p16蛋白的表达情况,并分析Gal-1、p16蛋白表达与宫颈癌患者临床特征的关系。结果宫颈癌组织中Gal-1、p16蛋白的阳性表达率均明显高于CIN组织(P﹤0.01);国际妇产科联盟(FIGO)分期为Ⅲ～Ⅳ期、有淋巴结转移、肿瘤分化程度为低分化的宫颈癌患者宫颈癌组织中Gal-1蛋白阳性表达率高于FIGO分期为Ⅰ～Ⅱ期、无淋巴结转移、肿瘤分化程度为中高分化的患者(P﹤0.05);肿瘤分化程度为低分化的宫颈癌患者宫颈癌组织中p16蛋白的阳性表达率明显高于肿瘤分化程度为中高分化的患者(P﹤0.01)。结论宫颈癌组织中Gal-1、p16蛋白的表达上调,并且与宫颈癌的恶性程度有一定的关系。
Objective To investigate the associations between the expression of galectin-1(Gal-1) and p16 protein and clinical characteristics of patients with cervical cancer. Method Cervical cancer tissue samples from 100 patients with cervical cancer and cervical intraepithelial neoplasia(CIN) tissue samples from 100 patients with CIN were collected. Immunohistochemical staining was employed to detect the expression of Gal-1 and p16 in tissues from both cervical cancer and CIN patients, and the relationship between Gal-1 and p16 expression and clinical features of cervical cancer patients was analyzed. Result The positive rate of Gal-1 and p16 in cervical cancer tissues were significantly higher than those in CIN tissues(P<0.01). The positive rate of Gal-1 in cervical cancer tissues with International Federation of Gynecology and Obstetrics(FIGO) stage III-IV stage, lymph node metastasis and poorly differentiated is significantly higher than that from tissues with FIGO stage I-II stage, no lymph node metastasis, and well differentiated(P<0.05); The positive rate of p16 in cervical cancer tissues from patients with poorly differentiated cervical cancer was significantly higher than that in patients with well differentiated tissues(P<0.01). Conclusion Gal-1 and p16 expression is up-regulated in cervical cancer tissues and relate to the malignancy of cervical cancer to some extent.
Objective To investigate the associations between the expression of galectin-1(Gal-1) and p16 protein and clinical characteristics of patients with cervical cancer. Method Cervical cancer tissue samples from 100 patients with cervical cancer and cervical intraepithelial neoplasia(CIN) tissue samples from 100 patients with CIN were collected. Immunohistochemical staining was employed to detect the expression of Gal-1 and p16 in tissues from both cervical cancer and CIN patients, and the relationship between Gal-1 and p16 expression and clinical features of cervical cancer patients was analyzed. Result The positive rate of Gal-1 and p16 in cervical cancer tissues were significantly higher than those in CIN tissues(P<0.01). The positive rate of Gal-1 in cervical cancer tissues with International Federation of Gynecology and Obstetrics(FIGO) stage III-IV stage, lymph node metastasis and poorly differentiated is significantly higher than that from tissues with FIGO stage I-II stage, no lymph node metastasis, and well differentiated(P<0.05); The positive rate of p16 in cervical cancer tissues from patients with poorly differentiated cervical cancer was significantly higher than that in patients with well differentiated tissues(P<0.01). Conclusion Gal-1 and p16 expression is up-regulated in cervical cancer tissues and relate to the malignancy of cervical cancer to some extent.
引文
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[15]张卉,杨德生,马许辉,等.半乳糖凝集素-1在结肠腺癌组织的表达及临床意义[J].中华实验外科杂志,2016,33(6):1649-1652.
[16]胡伟伟,李伟,袁方,等.半乳糖凝集素-1和α心肌1在脑胶质瘤血清中水平及其临床意义[J].实用癌症杂志,2017,32(5):716-718.
[2]张昕蕾,张军,蔡有芹,等.腹腔镜手术治疗108例宫颈癌的临床分析[J].中国微创外科杂志,2016,16(11):980-982.
[3]姬媛媛,王志东,陈海燕,等.IGF-Ⅱ对人肝癌细胞HepG2中抑癌基因p16和p53表达的影响[J].现代肿瘤医学,2017,25(12):1853-1858.
[4]朱玉洁,仲琳,杨军.半乳糖凝集素-3、白介素-6、单核细胞趋化蛋白-1与动脉粥样硬化斑块稳定性的相关性研究[J].解放军医药杂志,2017,29(12):39-41.
[5]罗乐,李秋江,龙兴霞,等.半乳糖凝集素3在膀胱癌组织中表达和临床意义及其对T24细胞恶性生物学行为的影响[J].中国肿瘤生物治疗杂志,2018,25(5):503-508.
[6]吴银霞,周广臣,牛荃,等.半乳糖凝集素-1在膀胱癌中的表达及临床意义[J].现代泌尿外科杂志,2018,23(4):259-262.
[7]谢幸,苟文丽.妇产科学[M].8版.北京:人民卫生出版社,2014.
[8]黄桢,王艳林,黄利鸣.长链非编码RNA与宫颈癌关系的研究进展[J].生命的化学,2018,38(2):185-190.
[9]高莹莹.p16/Ki-67双染检测助力宫颈癌前病变诊断[J].临床与实验病理学杂志,2016,32(9):966.
[10]Yildirim M,Müller von der Grün J,Winkelmann R,et al.Combined p16 and p53 expression in cervical cancer of unknown primary and other prognostic parameters:a single-center analysis[J].Strahlenther Onkol,2017,193(4):305-314.
[11]万玲玲,梁芸,贺宇彤,等.血浆p16基因甲基化联合p53抗体在非小细胞肺癌诊断中的价值[J].肿瘤学杂志,2017,23(5):372-376.
[12]Tong R,Yang Q,Wang C,et al.OVCA1 expression and its correlation with the expression levels of cyclin D1 and p16 in cervical cancer and intraepithelial neoplasia[J].Oncol Lett,2017,13(5):2929-2936.
[13]Yildirim M,Müller von der Grün J,Winkelmann R,et al.Erratum to:combined p16 and p53 expression in cervical cancer of unknown primary and other prognostic parameters[J].Strahlenther Onkol,2017,193(8):682.
[14]张自辉,叶红.半乳糖凝集素-1与恶性肿瘤的关系研究进展[J].海南医学,2014,25(21):3195-3197.
[15]张卉,杨德生,马许辉,等.半乳糖凝集素-1在结肠腺癌组织的表达及临床意义[J].中华实验外科杂志,2016,33(6):1649-1652.
[16]胡伟伟,李伟,袁方,等.半乳糖凝集素-1和α心肌1在脑胶质瘤血清中水平及其临床意义[J].实用癌症杂志,2017,32(5):716-718.