酸酯碱成分结合指纹图谱优选盐黄柏炮制工艺
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摘要
通过测定三大类成分含量,结合盐黄柏指纹图谱优选盐黄柏炮制工艺。采用HLPC-DAD法测定盐黄柏中6种生物碱以及2种酸类、1种内酯,共9种,3大类成分含量。以AHP法确定各指标成分权重,综合评分结合指纹图谱,通过单因素及正交设计实验,定性定量的分析炮制温度、炮制时间、炒药量对盐黄柏炮制的影响,优选盐黄柏炮制工艺。优选炒制工艺为:180℃,20 min,300 g药材(占容器体积1/4)。三大类成分结合指纹图谱优选的盐黄柏炮制工艺稳定、可行;此外,该方法可用于类似工艺的筛选。
To research the HPLC fingerprint and three major chemical components changes of Cortex Phellodendri,HPLC.was ued to determine three kinds compositions in Phellodendron chinense Schneid bark,including six alkaloids,two acids and an ester.AHP was adopted to determine the weight of each index.Comprehensive score combined with HLPC Fingerprint used to optimize the Processing of Stir-bake with Salt-water for Phellodendron chinense Schneid by the design of single factor and orthogonal test.Qualitative and quantitative analysis of the effects of processing temperature,processing time and the amount of fried.The Comprehensive Study of HLPC Fingerprint and Three Major Chemical Components to Optimize the Processing of Stir-bake with Salt-water for Phellodendron chinense Schneid bark was as following: 180℃,20 min,300 g(volume 1/4 of container).The processing technology of Phellodendron chinense bark was stable and feasible optimized by three kinds of ingredients combined with HLPC fingerprints,can be used for mass production.In addition,this method can be used for screening similar processes.
To research the HPLC fingerprint and three major chemical components changes of Cortex Phellodendri,HPLC.was ued to determine three kinds compositions in Phellodendron chinense Schneid bark,including six alkaloids,two acids and an ester.AHP was adopted to determine the weight of each index.Comprehensive score combined with HLPC Fingerprint used to optimize the Processing of Stir-bake with Salt-water for Phellodendron chinense Schneid by the design of single factor and orthogonal test.Qualitative and quantitative analysis of the effects of processing temperature,processing time and the amount of fried.The Comprehensive Study of HLPC Fingerprint and Three Major Chemical Components to Optimize the Processing of Stir-bake with Salt-water for Phellodendron chinense Schneid bark was as following: 180℃,20 min,300 g(volume 1/4 of container).The processing technology of Phellodendron chinense bark was stable and feasible optimized by three kinds of ingredients combined with HLPC fingerprints,can be used for mass production.In addition,this method can be used for screening similar processes.
引文
1 Chinese Pharmacopoeia Commission.Pharmacopoeia of the Peoples Republic of China VolⅠ(中华人民共和国药典:第一部)[M].Beijing:China Medical Science Press,2015:305.
2 Wang M,Ji TF,Yang JB,et al.Studies on the Chemical Constituents of Phellodendron chinense[J].Chin Med Mat(中药材),2009,2:208-210.
3 Yu YJ,Li JS,Chen ZP,et al.The comparative study of HPLCfingerprint between Cortex Phellodendri and Cortex Phellodendri processed products[J].Guid J Tradit Chin Med Pharm(中医药导报),2011,17(9):76-78.
4 Lin F,Wang YH,Wang L,et al.Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2012,13:78-82.
5 Cheng ZQ,Shi CJ,Liu XM,et al.Application of Golden section method with Dynamic Optimization in Extraction Process Optimization of Salt Cortex Phellodendri[J].Nat Prod Res Dev(天然产物研究与开发),2018,30:1030-1036.
6 Wu SS,Hu CJ,Lyu FF,et al.Determination of five alkaloids from Phellodendri cortex by HPLCt[J].Chin Tradit Pat Mde(中成药),2010,32:443-447.
7 Qi DL,Jia TZ,Lian L.Change of constituents in cortex Phellodendri after processingt[J].Chin Tradit Pat Mde(中成药),2010,32:443-447.
8 Yang AX,Yi K,Yi YD,et al.Study on Water Extraction Process of Traditional Chinese Medicine Qubai Granule by Orthogonal Test[J].China Pharm(中国药师),2016,19:1849-1852.
2 Wang M,Ji TF,Yang JB,et al.Studies on the Chemical Constituents of Phellodendron chinense[J].Chin Med Mat(中药材),2009,2:208-210.
3 Yu YJ,Li JS,Chen ZP,et al.The comparative study of HPLCfingerprint between Cortex Phellodendri and Cortex Phellodendri processed products[J].Guid J Tradit Chin Med Pharm(中医药导报),2011,17(9):76-78.
4 Lin F,Wang YH,Wang L,et al.Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2012,13:78-82.
5 Cheng ZQ,Shi CJ,Liu XM,et al.Application of Golden section method with Dynamic Optimization in Extraction Process Optimization of Salt Cortex Phellodendri[J].Nat Prod Res Dev(天然产物研究与开发),2018,30:1030-1036.
6 Wu SS,Hu CJ,Lyu FF,et al.Determination of five alkaloids from Phellodendri cortex by HPLCt[J].Chin Tradit Pat Mde(中成药),2010,32:443-447.
7 Qi DL,Jia TZ,Lian L.Change of constituents in cortex Phellodendri after processingt[J].Chin Tradit Pat Mde(中成药),2010,32:443-447.
8 Yang AX,Yi K,Yi YD,et al.Study on Water Extraction Process of Traditional Chinese Medicine Qubai Granule by Orthogonal Test[J].China Pharm(中国药师),2016,19:1849-1852.