不同添加剂对蛹虫草子实体生长情况及虫草素的影响
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摘要
为了提高人工栽培过程中蛹虫草的子实体产量以及主要活性成分虫草素的含量,本实验通过分别添加不同剂量的2,4-D、亚硒酸钠、柠檬酸三铵、以及不同的维生素(VB1、VB3、VB6和叶酸),分析其对蛹虫草生长分化及虫草素合成的影响.结果表明:能增加蛹虫草子实体发育和产量的添加剂质量体积比为:2,4-D 0.1 g/L、亚硒酸钠25 mg/L、柠檬酸三铵1.6 g/L,而维生素的影响不大;亚硒酸钠、柠檬酸三铵对虫草素含量影响不明显,但2,4-D 0.4 g/L和VB3 1.0 g/L最能促进虫草素的形成.
In order to improve the quality of Cordyceps militaris fruiting bodies and the main active ingredients cordycepin in the artificial cultivation process,we carried out the experiments by adding different doses of 2,4-D,sodium selenite,triammonium citrate,and different vitamins(VB1,VB3,VB6 and folic acid) to analyze its effects on the growth and differentiation of C.militaris and cordycepin synthesis.The results showed that 2,4-D 0.1 g/L,sodium selenite 25 mg/L and triammonium citrate 1.6 g/L could promote the developmemt and production of fruit body,while Vitamins had little effect.The effects on cordycepin content adding sodium selenite and triammonium citrate were not obvious.But 2,4-D 0.4 g/L and VB3 1.0 g/L were the most suitable to promote formation of cordycepin.
In order to improve the quality of Cordyceps militaris fruiting bodies and the main active ingredients cordycepin in the artificial cultivation process,we carried out the experiments by adding different doses of 2,4-D,sodium selenite,triammonium citrate,and different vitamins(VB1,VB3,VB6 and folic acid) to analyze its effects on the growth and differentiation of C.militaris and cordycepin synthesis.The results showed that 2,4-D 0.1 g/L,sodium selenite 25 mg/L and triammonium citrate 1.6 g/L could promote the developmemt and production of fruit body,while Vitamins had little effect.The effects on cordycepin content adding sodium selenite and triammonium citrate were not obvious.But 2,4-D 0.4 g/L and VB3 1.0 g/L were the most suitable to promote formation of cordycepin.
引文
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[11] 袁泰斗,陈月仍,吴坤林.影响蛹虫草子实体产量及质量的因素研究[J].广东农业科学,2006(10):22-23.
[12] 李琴,张弛.不同原料来源的虫草素的提取研究概况[J].湖北民族学院学报(自然科学版),2013,31(2):149-152.
[13] 孙润生,孙冬梅,张杰,等.蛹虫草产虫草素培养基优化[J].黑龙江八一农垦大学学报,2018,30(5):92-97.
[2] 李祝,刘爱英,梁宗琦.虫草菌素的生物活性及检测方法[J].食用菌学报,2002,9(1):57-62.
[3] 彭国平,李红阳,袁永泰.冬虫夏草与人工蛹虫草的成分比较[J].南京中医药大学学报,1996,12(5):26-27.
[4] 肖正华,李再新,李建章,等.添加剂对蛹虫草子实体生长分化影响的研究[J].食品与发酵科技,2010,(3):60-64.
[5] 袁泰斗,陈月仍,吴坤林.影响蛹虫草子实体产量及质量的因素研究[J].广东农业科学,2006(10):22-23.
[6] 孟小丽.蛹虫草复壮的人工栽培和退化的ISSR初步分析[D].昆明:西南林业大学,2017.
[7] 阮元,马进川,薛元,等.维生素B1、B6和生长激素2,4-D对蛹虫草液体发酵虫草素产量的影响[J].菌物学报,2014,33(2):477-482.
[8] 郭明敏.蛹虫草子实体虫草素、腺苷含量的影响因素研究[D].沈阳:沈阳师范大学,2016.
[9] 肖正华,李再新,李建章,等.添加剂对蛹虫草子实体生长分化影响的研究[J].食品与发酵科技,2010(3):60-64.
[10] 方华舟,肖习明.不同光照强度对蛹虫草主要活性成分的影响规律[J].北方园艺,2017(24):170-177.
[11] 袁泰斗,陈月仍,吴坤林.影响蛹虫草子实体产量及质量的因素研究[J].广东农业科学,2006(10):22-23.
[12] 李琴,张弛.不同原料来源的虫草素的提取研究概况[J].湖北民族学院学报(自然科学版),2013,31(2):149-152.
[13] 孙润生,孙冬梅,张杰,等.蛹虫草产虫草素培养基优化[J].黑龙江八一农垦大学学报,2018,30(5):92-97.