电针对去卵巢大鼠骨密度及生物力学的影响
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摘要
目的研究电针对去卵巢骨质疏松模型大鼠骨密度(BMD)、生物力学的影响。方法选取60只雌性SD大鼠,随机分为空白组、模型组、假手术组、药物组、电针组,每组12只。空白组不做任何处理;假手术组只暴露双侧卵巢但不切除;其余3组行双侧切卵巢法建立骨质疏松模型大鼠。造模13周后开始治疗,电针组取"肾俞"、"脾俞"、"三阴交"、"足三里",每次电针刺激15 min(断续波,2 Hz,1.0m A)并灌服与药物组相同体积的蒸馏水,每日1次,连续治疗5 d,间隔2 d,20 d为1个疗程,共3个疗程;药物组大鼠以戊酸雌二醇水溶液(0.02 mg/mL,1 mL/100 g体质量)灌胃,疗程同电针组;空白组不予处理,常规饲养;模型组、假手术组大鼠灌服与药物组相同体积的蒸馏水,疗程同电针组。治疗结束后麻醉处死大鼠,分离股骨和第4腰椎,双能X射线骨密度检测仪检测股骨和腰椎BMD;三点弯曲法和压缩法检测股骨和腰椎生物力学。结果与假手术组相比,模型组大鼠股骨和第四腰椎离体BMD、股骨和第四腰椎的最大载荷及弹性模量均显著性降低(P<0.01);与模型组相比,电针组、药物组大鼠股骨和第四腰椎离体BMD、股骨和第四腰椎的最大载荷及弹性模量均显著性升高(P<0.01);电针组、药物组大鼠股骨和第四腰椎离体BMD、股骨和第四腰椎的最大载荷及弹性模量均显著性低于假手术组(P<0.01)。结论针刺能有效增加大鼠骨密度,阻止骨量降低,减缓骨力学的退变,增强骨强度,对绝经后骨质疏松症具有一定的治疗作用。
Objective To observe the effect of electro-acupuncture on bone mineral density( BMD) and biomechanics in ovariectomized rats. Methods Sixty female SD rats were randomly divided into control group,model group,sham operation group,medicine group,and electro-acupuncture group,with 12 rats in each group. Rats in control group received no treatment. The ovary of rats in sham operation group were only exposed but non-resected. The bilateral ovariectomy was performed to build postmenopausal osteoporosis model in rats of the rest groups. Thirteen weeks later,rats in electro-acupuncture group received electro-acupuncture at Shenshu( BL23),Pishu( BL20),Sanyinjiao( SP6),and Zusanli( ST36) points for 15 minutes( discontinuous wave,2 Hz,1. 0 mA),and fed with distilled water equally once a day for 5 days. One course lasted for 20 days with a 2-day interval,and the treatment lasted for 3 courses. Rats in medicine group received estradiol valerate aqueous solution( 0. 02 mg/mL,1 mL/100 g weight) by gavage. Rats in control group and the rest groups were fed with an equal volume of distilled water. After the treatment,Rats were sacrificed under anesthesia. The femurs and the fourth lumbar vertebrae were isolated for BMD examination and biomechanical test with femur air bending and lumbar compression. Results Compared with sham operation group,BMD,the maximum load,and elastic modulus of the femurs and the fourth lumbar vertebrae in model groups decreased significantly( P < 0. 01). Compared with the model groups,BMD,the maximum load,and elastic modulus of the femurs and the fourth lumbar vertebrae in medicine group and electro-acupuncture group increased significantly( P < 0. 01). BMD,the maximum load,and elastic modulus of the femurs and the fourth lumbar vertebrae in electro-acupuncture group and medicine group were significantly lower than those in sham operation group( P < 0. 01). Conclusion Electro-acupuncture effectively increases BMD,prevents bone loss and mechanics degeneration,and benefits the treatment of postmenopausal osteoporosis.
Objective To observe the effect of electro-acupuncture on bone mineral density( BMD) and biomechanics in ovariectomized rats. Methods Sixty female SD rats were randomly divided into control group,model group,sham operation group,medicine group,and electro-acupuncture group,with 12 rats in each group. Rats in control group received no treatment. The ovary of rats in sham operation group were only exposed but non-resected. The bilateral ovariectomy was performed to build postmenopausal osteoporosis model in rats of the rest groups. Thirteen weeks later,rats in electro-acupuncture group received electro-acupuncture at Shenshu( BL23),Pishu( BL20),Sanyinjiao( SP6),and Zusanli( ST36) points for 15 minutes( discontinuous wave,2 Hz,1. 0 mA),and fed with distilled water equally once a day for 5 days. One course lasted for 20 days with a 2-day interval,and the treatment lasted for 3 courses. Rats in medicine group received estradiol valerate aqueous solution( 0. 02 mg/mL,1 mL/100 g weight) by gavage. Rats in control group and the rest groups were fed with an equal volume of distilled water. After the treatment,Rats were sacrificed under anesthesia. The femurs and the fourth lumbar vertebrae were isolated for BMD examination and biomechanical test with femur air bending and lumbar compression. Results Compared with sham operation group,BMD,the maximum load,and elastic modulus of the femurs and the fourth lumbar vertebrae in model groups decreased significantly( P < 0. 01). Compared with the model groups,BMD,the maximum load,and elastic modulus of the femurs and the fourth lumbar vertebrae in medicine group and electro-acupuncture group increased significantly( P < 0. 01). BMD,the maximum load,and elastic modulus of the femurs and the fourth lumbar vertebrae in electro-acupuncture group and medicine group were significantly lower than those in sham operation group( P < 0. 01). Conclusion Electro-acupuncture effectively increases BMD,prevents bone loss and mechanics degeneration,and benefits the treatment of postmenopausal osteoporosis.
引文
[1]李金艳,陈颖,唐艳丽,等.针灸治疗骨质疏松症效应机制研究进展.中国骨质疏松杂志,2014,20(3):315-321.Li JY,Chen Y,Tang YL,et al.Mechanism of the effect of acupuncture treatment of osteoporosis.Chinese Journal of Osteoporosis,2014,20(3):315-321.
[2]康轶鑫,王道全,刘忠厚.针灸治疗骨质疏松症的实验研究进展.中国骨质疏松杂志,2011,17(1):73-79.Kang YX,Wang DQ,Liu ZH.The experimental research progress of acupuncture therapy for the treatment of osteoporosis.Chinese Journal of Osteoporosis,2011,17(1):73-79.
[3]中国老年学学会骨质疏松委员会.中国人群骨质疏松症防治手册2010版.中国骨质疏松杂志,2010,16(增刊-1):153.Osteoporosis Committee of the Gerontological Society of China.Handbook of prevention and treatment of osteoporosis in Chinese population,2010 Edition.Chinese Journal of Osteoporosis,2010,16(Supplement-1):153.
[4]钟旋,赵利华,范郁山.针灸防治绝经后骨质疏松症的研究近况.亚太传统医药,2008,4(4):63-65.Zhong X,Zhao LH,Fan YS.Recent improvement in the Study of Acupuncture for prevention and treatment of postmenopausal osteoporosis.Asia-Pacific Traditional Medicine,2008,4(4):63-65.
[5]全国卫生专业技术资格考试专家委员会.康复医学与治疗技术.北京:人民卫生出版社,2008:282-286.Expert Committee of nationwide Health professional and technical qualification examination.Rehabilitation medicine and treatment technology.Beijing:People's Medical Publishing House.2008:282-286.
[6]王亚军,鞠大宏.针灸治疗妇女绝经后骨质疏松的实验研究进展述评.按摩与康复医学,2012,3(3):57-59.Wang YJ,Ju DH.Review of the experimental research progress of Acupunture therapy in postmenopausal osteoporosis.Chinese Manipulation&Rehabilitation Medicine,2012,3(3):57-59.
[7]徐亚莉,金建军,徐登玉.针灸治疗骨质疏松症研究进展.甘肃中医学院学报,2006,23(2):53-56.Xu YL,Jin JJ,Xu DY.The experimental research progress of acupuncture therapy for the treatment of osteoporosis.Journal of Gansu College of Traditional Chinese Medicine,2006,23(2):53-56.
[8]鞠大宏,于福禄,张丽坤,等.滋阴补肾法对卵巢切除所致骨质疏松大鼠成骨细胞COX-2蛋白和mRNA表达的影响.中国中医基础医学杂志,2006,12(12):918-920.Ju DH,Yu FL,Zhang LK,et al.The effect of the method of nourishing the kidney-yin on the expression of the osteoblast COX-2 and mRNA in the ovariectomy-induced osteoporosis rats.Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2006,12(12):918-920.
[9]郭义.实验针灸学.北京:中国中医药出版社,2008:1.Guo Y.Experimental acupuncture.Beijing:China Press of Traditional Chinese Medicine,2008:1.
[10]欧阳刚,唐曦,莫非,等.针刺干预对去卵巢大鼠血清胃饥饿素和骨密度的影响.针刺研究,2013,38(4):277-280.Ou YG,Tang X,Mo F,et al.Influence of electroacupuncture intervention on serum ghrelin content and bone mineral density in ovariectomized rats.Acupuncture Research,2013,38(4):277-280.
[11]鲍圣涌,张少君,林婉娟,等.电针对去卵巢骨质疏松大鼠骨代谢生化指标及肿瘤坏死因子-α和骨胶原代谢生化指标的影响.中国针灸,2012,32(12):1108-1112.Bao SY,Zhang SJ,Lin WJ,et al.Effect of electroacupuncture on the biochemical indices of bone and bone collagen metabolism and TNF-αin osteoporosis model rats without ovaries.Chinese Acupuncture&Moxibustion,2012,32(12):1108-1112.
[12]张瑶,朱旭,刘菊,等.二仙汤对去卵巢骨质疏松模型大鼠血清I型胶原氨基端延长肽影响的实验研究.广西医学,2014,36(11):1593-1595.Zhang Y,Zhu X,Liu J,et al.Experimental study the effect of erxian decoction on serum amino terminal extension peptide of type I collagen in osteoporosis model rats without ovaries.Guang Xi Medical Journa,2014,36(11):1593-1595.
[13]杨路昕,蔡辉.原发性骨质疏松症的中医病因病机研究进展.河北中医,2009,31(3):467-469.Yang LX,Cai H.The research progress of TCM etiology and pathogenesis on primary osteoporosis.Hebei Journal of Traditional Chinese Medicine,2009,31(3):467-469.
[14]罗成斌,蒋春彦,朱珠,等.针刺对实验性骨质疏松大鼠股骨骨折的预防作用.中国骨质疏松杂志,2017,23(1):102-106.Luo CB,Jiang CY,Zhu Z,et al.The effective of acupuncture on the prevention of osteoporotic femoral fraction in experimental osteoporosis rats.Chinese Journal of Osteoporosis,2017,23(1):102-106.
[15]毕成林,韩万伟,唐翔,等.骨质疏松引发老年患者发生骨折的影响因素分析.中国矫形外科杂志,2015,23(4):368-370.Bi CL,Han WW,Tang X,et al.Analysis the influence factors of fracture in elderly patients with osteoporosis,The Orthopedic Journal of China,2015,23(4):368-370.
[16]张伟.绝经后股骨颈骨折股骨头骨铁及二价金属元素含量与骨密度相关性研究.苏州大学,2014.Zhang W.The association of bone iron and other divalent metal elements contents with bone mineral density in postmenopausal women with femoral neck fragility fracture.Suzhou University,2014.
[17]耿力,姚珍薇,骆建云,等.腰椎和股骨近端骨密度测定在原发性骨质疏松症诊断中的相关性分析.重庆医科大学学报,2007,32(6):592-596,605.Geng L,Yao ZW,Luo JY,et al.Correlation analysis of measuring bone mineral density of lumbar and proximal femur in the diagnosis of primary osteoporosis.Journal of Chongqing Medical University,2007,32(6):592-596,605.
[18]朱慧锋,程金海,王珠美,等.去卵巢大鼠干预后骨生物力学研究.中医正骨,2009,21(8):5-7.Zhu HF,Cheng JH,Wang ZM,et al.The study of Bone biomechanical in Prognosis ovariectomized rats.The Journal of Traditional Chinese Orthopedics and Traumatology,2009,21(8):5-7.
[19]马界,罗永芬,蓝肇熙,等.针灸结合骨立对去势大鼠骨质疏松症骨密度及生物力学影响的研究.四川中医,2008,26(8):9-11.Ma J,Luo YF,Lan ZX,et al.Study on the effects of acupuncture and Moxibustion combined with guli method on the mineral density and biomechanics of overiectomized rats with osteoporosis.Journal of Sichuan of Traditional Chinese Medicine,2008,26(8):9-11.
[20]王亚军,刘梅洁,鞠大宏,等.针刺对去卵巢骨质疏松模型大鼠骨密度、骨形态计量学的影响.中国中医基础医学杂志,2015,21(7):857-859,871.Wang YJ,Liu MJ,Ju DH,et al.Influence of acupuncture intervention on bone mineral density and bone histomorphometry in ovariectomized rats.Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2015,21(7):857-859,871.
[21]罗成斌,徐金龙,杨增荣,等.整体调节针法治疗原发性骨质疏松症的临床研究.中国骨质疏松杂志,2016,22(11):1459-1465.Luo CB,Xu JL,Yang ZR,et al.Clinical study of the treatment of primary osteoporosis with overall regulation of acupuncture.Chinese Journal of Osteoporosis,2016,22(11):1459-1465.
[2]康轶鑫,王道全,刘忠厚.针灸治疗骨质疏松症的实验研究进展.中国骨质疏松杂志,2011,17(1):73-79.Kang YX,Wang DQ,Liu ZH.The experimental research progress of acupuncture therapy for the treatment of osteoporosis.Chinese Journal of Osteoporosis,2011,17(1):73-79.
[3]中国老年学学会骨质疏松委员会.中国人群骨质疏松症防治手册2010版.中国骨质疏松杂志,2010,16(增刊-1):153.Osteoporosis Committee of the Gerontological Society of China.Handbook of prevention and treatment of osteoporosis in Chinese population,2010 Edition.Chinese Journal of Osteoporosis,2010,16(Supplement-1):153.
[4]钟旋,赵利华,范郁山.针灸防治绝经后骨质疏松症的研究近况.亚太传统医药,2008,4(4):63-65.Zhong X,Zhao LH,Fan YS.Recent improvement in the Study of Acupuncture for prevention and treatment of postmenopausal osteoporosis.Asia-Pacific Traditional Medicine,2008,4(4):63-65.
[5]全国卫生专业技术资格考试专家委员会.康复医学与治疗技术.北京:人民卫生出版社,2008:282-286.Expert Committee of nationwide Health professional and technical qualification examination.Rehabilitation medicine and treatment technology.Beijing:People's Medical Publishing House.2008:282-286.
[6]王亚军,鞠大宏.针灸治疗妇女绝经后骨质疏松的实验研究进展述评.按摩与康复医学,2012,3(3):57-59.Wang YJ,Ju DH.Review of the experimental research progress of Acupunture therapy in postmenopausal osteoporosis.Chinese Manipulation&Rehabilitation Medicine,2012,3(3):57-59.
[7]徐亚莉,金建军,徐登玉.针灸治疗骨质疏松症研究进展.甘肃中医学院学报,2006,23(2):53-56.Xu YL,Jin JJ,Xu DY.The experimental research progress of acupuncture therapy for the treatment of osteoporosis.Journal of Gansu College of Traditional Chinese Medicine,2006,23(2):53-56.
[8]鞠大宏,于福禄,张丽坤,等.滋阴补肾法对卵巢切除所致骨质疏松大鼠成骨细胞COX-2蛋白和mRNA表达的影响.中国中医基础医学杂志,2006,12(12):918-920.Ju DH,Yu FL,Zhang LK,et al.The effect of the method of nourishing the kidney-yin on the expression of the osteoblast COX-2 and mRNA in the ovariectomy-induced osteoporosis rats.Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2006,12(12):918-920.
[9]郭义.实验针灸学.北京:中国中医药出版社,2008:1.Guo Y.Experimental acupuncture.Beijing:China Press of Traditional Chinese Medicine,2008:1.
[10]欧阳刚,唐曦,莫非,等.针刺干预对去卵巢大鼠血清胃饥饿素和骨密度的影响.针刺研究,2013,38(4):277-280.Ou YG,Tang X,Mo F,et al.Influence of electroacupuncture intervention on serum ghrelin content and bone mineral density in ovariectomized rats.Acupuncture Research,2013,38(4):277-280.
[11]鲍圣涌,张少君,林婉娟,等.电针对去卵巢骨质疏松大鼠骨代谢生化指标及肿瘤坏死因子-α和骨胶原代谢生化指标的影响.中国针灸,2012,32(12):1108-1112.Bao SY,Zhang SJ,Lin WJ,et al.Effect of electroacupuncture on the biochemical indices of bone and bone collagen metabolism and TNF-αin osteoporosis model rats without ovaries.Chinese Acupuncture&Moxibustion,2012,32(12):1108-1112.
[12]张瑶,朱旭,刘菊,等.二仙汤对去卵巢骨质疏松模型大鼠血清I型胶原氨基端延长肽影响的实验研究.广西医学,2014,36(11):1593-1595.Zhang Y,Zhu X,Liu J,et al.Experimental study the effect of erxian decoction on serum amino terminal extension peptide of type I collagen in osteoporosis model rats without ovaries.Guang Xi Medical Journa,2014,36(11):1593-1595.
[13]杨路昕,蔡辉.原发性骨质疏松症的中医病因病机研究进展.河北中医,2009,31(3):467-469.Yang LX,Cai H.The research progress of TCM etiology and pathogenesis on primary osteoporosis.Hebei Journal of Traditional Chinese Medicine,2009,31(3):467-469.
[14]罗成斌,蒋春彦,朱珠,等.针刺对实验性骨质疏松大鼠股骨骨折的预防作用.中国骨质疏松杂志,2017,23(1):102-106.Luo CB,Jiang CY,Zhu Z,et al.The effective of acupuncture on the prevention of osteoporotic femoral fraction in experimental osteoporosis rats.Chinese Journal of Osteoporosis,2017,23(1):102-106.
[15]毕成林,韩万伟,唐翔,等.骨质疏松引发老年患者发生骨折的影响因素分析.中国矫形外科杂志,2015,23(4):368-370.Bi CL,Han WW,Tang X,et al.Analysis the influence factors of fracture in elderly patients with osteoporosis,The Orthopedic Journal of China,2015,23(4):368-370.
[16]张伟.绝经后股骨颈骨折股骨头骨铁及二价金属元素含量与骨密度相关性研究.苏州大学,2014.Zhang W.The association of bone iron and other divalent metal elements contents with bone mineral density in postmenopausal women with femoral neck fragility fracture.Suzhou University,2014.
[17]耿力,姚珍薇,骆建云,等.腰椎和股骨近端骨密度测定在原发性骨质疏松症诊断中的相关性分析.重庆医科大学学报,2007,32(6):592-596,605.Geng L,Yao ZW,Luo JY,et al.Correlation analysis of measuring bone mineral density of lumbar and proximal femur in the diagnosis of primary osteoporosis.Journal of Chongqing Medical University,2007,32(6):592-596,605.
[18]朱慧锋,程金海,王珠美,等.去卵巢大鼠干预后骨生物力学研究.中医正骨,2009,21(8):5-7.Zhu HF,Cheng JH,Wang ZM,et al.The study of Bone biomechanical in Prognosis ovariectomized rats.The Journal of Traditional Chinese Orthopedics and Traumatology,2009,21(8):5-7.
[19]马界,罗永芬,蓝肇熙,等.针灸结合骨立对去势大鼠骨质疏松症骨密度及生物力学影响的研究.四川中医,2008,26(8):9-11.Ma J,Luo YF,Lan ZX,et al.Study on the effects of acupuncture and Moxibustion combined with guli method on the mineral density and biomechanics of overiectomized rats with osteoporosis.Journal of Sichuan of Traditional Chinese Medicine,2008,26(8):9-11.
[20]王亚军,刘梅洁,鞠大宏,等.针刺对去卵巢骨质疏松模型大鼠骨密度、骨形态计量学的影响.中国中医基础医学杂志,2015,21(7):857-859,871.Wang YJ,Liu MJ,Ju DH,et al.Influence of acupuncture intervention on bone mineral density and bone histomorphometry in ovariectomized rats.Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2015,21(7):857-859,871.
[21]罗成斌,徐金龙,杨增荣,等.整体调节针法治疗原发性骨质疏松症的临床研究.中国骨质疏松杂志,2016,22(11):1459-1465.Luo CB,Xu JL,Yang ZR,et al.Clinical study of the treatment of primary osteoporosis with overall regulation of acupuncture.Chinese Journal of Osteoporosis,2016,22(11):1459-1465.