一种抗疲劳软胶囊的制备及抗疲劳功效评价
|
摘要
研究抗疲劳软胶囊内容物的最佳制备工艺,并评价其抗疲劳功效。根据实验结果,内容物最佳工艺条件为:选择薄荷油与大豆油的复合油作为基质,薄荷油∶大豆油为1∶15;中药浸膏粉与基质的质量比为1∶2;浸膏粉粒度为160目;选择蜂蜡作为助悬剂,其用量为软胶囊内容物的4%;胶体磨在转速2 800 r/min下研磨20 min。该工艺制得的软胶囊内容物的均一性、流动性、黏度及稳定性均较好,所制得的软胶囊质量稳定;动物实验结果表明,各剂量组小鼠力竭游泳时间、肝糖原含量以及乳酸脱氢酶活性均显著提高(P<0.05),并且运动休息60 min后,各剂量组小鼠血清尿素含量显著降低,说明抗疲劳软胶囊能延长小鼠力竭游泳时间、提高肝糖原储存、提高乳酸脱氢酶活性。由此可见,抗疲劳软胶囊具有较好的缓解体力疲劳的功效。
Optimal preparating process of anti-fatigue soft capsule contents was studied and its anti-fatigue effect was ecaluated. According to the results of the experiment,the optimum conditions for the contents were as follows: the composite oil of peppermint oil and soybean oil was selected as the substrate, the ratio of peppermint oil to soybean oil was 1∶15; the mass ratio of traditional Chinese medicine extract powder to matrix was 1∶2; the particle size of the powder was 160 mesh; the beeswax is selected as the suspending agent, and the amount of the beeswax is 4% of the content of the soft capsule; the colloid mill was ground at a speed of 2 800 r/min for 20 min. The soft capsule contents obtained by the process have good homogeneity, fluidity, viscosity and stability, and the quality on the prepared soft capsules was stable; the animal experiment results showed that in each dose group, the exhaustive swimming time, the content of the liver glycogen of the mice and lactate dehydrogenase activity were significantly increased(P<0.05), and the serum urea content of the mice in each dose group decreased significantly after breaking for 60 mins, indicating that anti-fatigue soft capsule can prolong the exhaustive swimming time, increase liver glycogen storage and improve the activity on lactate dehydrogenase. It can be seen that the anti-fatigue soft capsule has good effect of relieving physical fatigue.
Optimal preparating process of anti-fatigue soft capsule contents was studied and its anti-fatigue effect was ecaluated. According to the results of the experiment,the optimum conditions for the contents were as follows: the composite oil of peppermint oil and soybean oil was selected as the substrate, the ratio of peppermint oil to soybean oil was 1∶15; the mass ratio of traditional Chinese medicine extract powder to matrix was 1∶2; the particle size of the powder was 160 mesh; the beeswax is selected as the suspending agent, and the amount of the beeswax is 4% of the content of the soft capsule; the colloid mill was ground at a speed of 2 800 r/min for 20 min. The soft capsule contents obtained by the process have good homogeneity, fluidity, viscosity and stability, and the quality on the prepared soft capsules was stable; the animal experiment results showed that in each dose group, the exhaustive swimming time, the content of the liver glycogen of the mice and lactate dehydrogenase activity were significantly increased(P<0.05), and the serum urea content of the mice in each dose group decreased significantly after breaking for 60 mins, indicating that anti-fatigue soft capsule can prolong the exhaustive swimming time, increase liver glycogen storage and improve the activity on lactate dehydrogenase. It can be seen that the anti-fatigue soft capsule has good effect of relieving physical fatigue.
引文
[1] 张宏霞,武宏伟,刘新民.抗疲劳药食两用中药现状分析[J].湖南中医药大学学报,2017,37(10):1166-1172.
[2] 张颖捷,杜万红.国内外抗疲劳研究进展[J].实用预防医学,2012,19(7):1112-1116.
[3] 张颖,廖森泰,王思远,等.动物源性抗疲劳肽研究与功能食品开发进展[J].农产品加工月刊,2017(13):67-71.
[4] Hsiao C Y ,Hsu Y J ,Tung Y T ,et al.Effects of antrodia camphorata and panax,ginseng supplementation on anti-fatigue properties in mice[J].Journal of Veterinary Medical Science,2018,80(2):284-291.
[5] 赵红丽,李菲,屈云萍,等.含膏粉软胶囊制备工艺研究[J].亚太传统医药,2017,13(8):37-38.
[6] 韩德权,孙庆申,王璐.一种醒脑生脉口服液的制备方法[P].中国专利:CN103495018A,2014.
[7] 王娟,贺莹莹,韦寿莲,等.黄皮果胶对力竭游泳小鼠的抗疲劳功效[J].中国科技论文,2015(24):2884-2887.
[8] 谭蓉,杨秀芳,孔俊豪,等.茶黄素软胶囊制备及功效评价[C]// 中国科协年会第20分会场:科技创新与茶产业发展论坛.2013.
[9] 程倩,董运海,张彦.玛咖西洋参锌片缓解体力疲劳的功能研究[J].食品科技,2018,43(6):104-107.
[10] 梁慧慧,萧伟,徐连明,等.银翘解毒软胶囊制备工艺研究[J].中草药,2013,44(8):965-969.
[11] 夏鸿林,胡翔.心脉通软胶嚢制备工艺及芦丁含量测定方法的确立[J].基因组学与应用生物学,2017,36(12):4893-4898.
[12] 张岗,唐平,田伟,等.杜仲叶软胶囊制备工艺研究[J].中国民族医药杂志,2015,21(6):66-67.
[13] 吴培源,郑建,俞晓红,等.山蜡梅软胶囊制备工艺研究[J].中国中医药现代远程教育,2014,12(23):114-115.
[14] 王珍婷.通便HD双歧杆菌结肠溶软胶囊的开发研究[D].黑龙江大学,2016.
[15] 王贤英,谢香菊,李胜容.金泽冠心软胶囊制剂工艺研究[J].中国药业,2013,22(15):59-61.
[16] Jiang X ,Chu Q ,Li L ,et al.The antifatigue activities of tuber melanosporum in a mouse model[J].Experimental and therapeutic medicine,2018,15(3):3066-3073.
[17] Schousboe A,Sickmann H M,Bak L K,et al.Neurong-lia interactions in glutamatergic neurotransmission:Roles of oxidative and glycolytic adenosine triphosphate as energy source[J].Journal of Neuroscience Research,2011,89(12):1926-1934.
[18] 林华庆,黄能章.舒胸软胶囊制备工艺的研究[J].中药材,2006,29(4):396-397.
[2] 张颖捷,杜万红.国内外抗疲劳研究进展[J].实用预防医学,2012,19(7):1112-1116.
[3] 张颖,廖森泰,王思远,等.动物源性抗疲劳肽研究与功能食品开发进展[J].农产品加工月刊,2017(13):67-71.
[4] Hsiao C Y ,Hsu Y J ,Tung Y T ,et al.Effects of antrodia camphorata and panax,ginseng supplementation on anti-fatigue properties in mice[J].Journal of Veterinary Medical Science,2018,80(2):284-291.
[5] 赵红丽,李菲,屈云萍,等.含膏粉软胶囊制备工艺研究[J].亚太传统医药,2017,13(8):37-38.
[6] 韩德权,孙庆申,王璐.一种醒脑生脉口服液的制备方法[P].中国专利:CN103495018A,2014.
[7] 王娟,贺莹莹,韦寿莲,等.黄皮果胶对力竭游泳小鼠的抗疲劳功效[J].中国科技论文,2015(24):2884-2887.
[8] 谭蓉,杨秀芳,孔俊豪,等.茶黄素软胶囊制备及功效评价[C]// 中国科协年会第20分会场:科技创新与茶产业发展论坛.2013.
[9] 程倩,董运海,张彦.玛咖西洋参锌片缓解体力疲劳的功能研究[J].食品科技,2018,43(6):104-107.
[10] 梁慧慧,萧伟,徐连明,等.银翘解毒软胶囊制备工艺研究[J].中草药,2013,44(8):965-969.
[11] 夏鸿林,胡翔.心脉通软胶嚢制备工艺及芦丁含量测定方法的确立[J].基因组学与应用生物学,2017,36(12):4893-4898.
[12] 张岗,唐平,田伟,等.杜仲叶软胶囊制备工艺研究[J].中国民族医药杂志,2015,21(6):66-67.
[13] 吴培源,郑建,俞晓红,等.山蜡梅软胶囊制备工艺研究[J].中国中医药现代远程教育,2014,12(23):114-115.
[14] 王珍婷.通便HD双歧杆菌结肠溶软胶囊的开发研究[D].黑龙江大学,2016.
[15] 王贤英,谢香菊,李胜容.金泽冠心软胶囊制剂工艺研究[J].中国药业,2013,22(15):59-61.
[16] Jiang X ,Chu Q ,Li L ,et al.The antifatigue activities of tuber melanosporum in a mouse model[J].Experimental and therapeutic medicine,2018,15(3):3066-3073.
[17] Schousboe A,Sickmann H M,Bak L K,et al.Neurong-lia interactions in glutamatergic neurotransmission:Roles of oxidative and glycolytic adenosine triphosphate as energy source[J].Journal of Neuroscience Research,2011,89(12):1926-1934.
[18] 林华庆,黄能章.舒胸软胶囊制备工艺的研究[J].中药材,2006,29(4):396-397.