遗传群体构建的分子标记检测体系
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摘要
为了建立遗传群体构建的分子标记检测体系,利用SSR分子标记技术构建了从DNA提取、引物多态性筛选、亲本和子代植株的PCR扩增、扩增产物的电泳检测等4步检测体系,实验中仅用1对位于大麦7H染色体上的引物HvWaxy4就对7个F1子代植株进行了鉴定,通过鉴定判断出其中3个植株为F1子代,4个单株为自花授粉产生的子代。表明SSR标记技术在验证遗传群体构建过程中F1杂交植株真伪的可行性,该方法适用于自花授粉作物的遗传群体构建过程中F1植株的真伪检测。
To establish a molecular marker detection system for genetic population construction, a four-step detection system was constructed using SSR molecular marker technology, including DNA extraction, primer polymorphism screening, PCR amplification of parents and offspring, and electrophoresis detection of amplified products. In the experiment, only one pair of primers HvWaxy4 located on barley 7 H chromosome was used to detect 7 F1 progenies, three of them were F1 progenies and four were self-pollinated progenies. The results indicated that SSR marker technology was feasible to verify the F1 hybrid plants in the process of genetic population construction, and the method was suitable to detect the authenticity of F1 plants in the process of self-pollinated crops.
To establish a molecular marker detection system for genetic population construction, a four-step detection system was constructed using SSR molecular marker technology, including DNA extraction, primer polymorphism screening, PCR amplification of parents and offspring, and electrophoresis detection of amplified products. In the experiment, only one pair of primers HvWaxy4 located on barley 7 H chromosome was used to detect 7 F1 progenies, three of them were F1 progenies and four were self-pollinated progenies. The results indicated that SSR marker technology was feasible to verify the F1 hybrid plants in the process of genetic population construction, and the method was suitable to detect the authenticity of F1 plants in the process of self-pollinated crops.
引文
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[21]王玲玲,陈东亮,黄丛林,等.SSR分子标记技术在植物研究中的应用[J].安徽农业科学,2017,45(36):123-126.
[22]席章营,张桂权. SSR标记及其在作物遗传育种中的应用[J].河南农业大学学报,2002,36(3):294-297.
[2] Zhou Z K, Jiang Y, Wang Z, et al. Resequencing 302 wild and cultivatedaccessions identifies genes related to domestication and improvementin soybean[J]. Nature Biotechnology, 2015,33:408-414.
[3] Lu F, Romay M C, Glaubitz J C, et al. High-resolution geneticmapping of maize pan-genome sequence anchors[J]. NatureCommunications, 2015,6:6914.
[4]张尧锋,张冬青,余华胜,等.基于极端混合池(BSA)全基因组重测序的甘蓝型油菜有限花序基因定位[J].中国农业科学,2018,51(16):3029-3039.
[5] Takagi H, Tamiru M, Abe A, et al. MutMap accelerates breeding of asalt-tolerant rice cultivar[J].Nature Biotechnology, 2015,33:445-449.
[6] Akagi H, Yokozeki Y, Inagaki A, et al. Microsatellite DNA markersfor rice chromosomes[J].Theor Appl Genet,1996,94:61-67.
[7]宋贤勇,包月红,杨俊涛,等.利用SSR标记鉴定甘蓝型油菜秦优11号种子纯度[J].分子植物育种,2010,8(3):497-500.
[8]高海娜,张恩慧,李殿荣,等.甘蓝一代杂种纯度SSR鉴定与田间鉴定的一致性研究[J].西北农林科技大学学报,2012,40(5):117-122.
[9]苗明军,李成琼,司军,等.结球甘蓝西园4号种子纯度的SSR鉴定[J].中国蔬菜,2011(2):53-55.
[10]管志坤,罗双霞,李艳霞,等.利用SSR标记鉴定油绿3号大白菜品种纯度[J].种子,2011,30(9):34-39.
[11]艾成祥,余贤美,刘庆忠,等.利用SSR标记鉴定西瓜杂交种纯度的研究[J].西北植物学报,2006,26(10):2006-2010.
[12]岳效飞,谢振文,王汉宁,等.应用SSR技术对三个优质水稻品种的指纹鉴定[J].中国农学通报,2007,23(11):72-76.
[13]彭锁云,庄杰云,颜启传,等.我国主要杂交水稻组合及其亲本SSR标记和纯度鉴定[J].中国水稻科学,2003,17(1):1-5.
[14]詹庆才.利用微卫星DNA标记进行杂交水稻种子纯度鉴定的研究[J].杂交水稻,2002,17(5):46-50.
[15]张敏,杨艳,康兆茹,等. EMS诱变南高丛越橘及抗旱突变体的筛选[J].西南师范大学学报:自然科学版,2011,36(3):132-137.
[16]王美芳,陈巨莲,原国辉,等.植物表面蜡质对植食性昆虫的影响研究进展[J].生态环境学报,2009(3):1155-1160.
[17]张正斌,山仑.小麦抗旱生理指标与叶片卷曲度和蜡质关系研究[J].作物学报,1998(5):608-612.
[18]徐亮.农作物总DNA的提取原理和主要方法概述.安徽农业科学,2011,39(16):9507-9509.
[19]黄留玉.PCR最新技术原理、方法及应用[M].北京:化学工业出版社,2003:254-257.
[20]王金胜,吕淑霞.基础生物化学[M].北京:中国农业出版社,2014:74-75.
[21]王玲玲,陈东亮,黄丛林,等.SSR分子标记技术在植物研究中的应用[J].安徽农业科学,2017,45(36):123-126.
[22]席章营,张桂权. SSR标记及其在作物遗传育种中的应用[J].河南农业大学学报,2002,36(3):294-297.