利用籼粳交RIL群体对水稻发芽期和苗期耐冷性的QTL分析
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摘要
为定位水稻发芽期和苗期耐冷性的QTL,鉴定新的耐冷基因位点,丰富水稻耐冷性的分子遗传基础。以籼型杂交稻恢复系品种泸恢99(Luhui 99,R99)和粳型超级稻品种沈农265(Shennong 265,SN265)杂交衍生的144个F8稳定遗传的重组自交系群体(Recombinant inbred lines,RILs)为试验材料,以低温条件下水稻种子发芽率和苗期叶片赤枯度为耐冷性鉴定标准,采用QTL Ici Mapping v3. 0软件基于完备复合区间作图法,对水稻发芽期和苗期耐冷性进行QTL分析。共检测到2个控制发芽期耐冷性的QTL和1个控制苗期耐冷性QTL,分别位于第3,5,9染色体上,命名为q LTG-3、q LTG-5、q SCT-9,3个QTL的LOD值分别为3. 60,2. 73,2. 52,加性效应为0. 09,-0. 10,-0. 09,可解释表型变异的11. 02%,14. 07%,12. 18%。其中,检测到控制发芽期耐冷性的q LTG-5位于分子标记R5M13～RS8,遗传距离约8.0 c M,该区间未见相关水稻耐冷性QTL的报道,可能是一个新的控制水稻发芽期耐冷性的QTL位点。
The aim of the study was to locate the QTL of rice cold tolerance at germination and seedling stages,to identify new locus of cold tolerance gene,and to enrich the molecular genetic basis of rice cold tolerance.144 F8 stable recombinant inbred lines( RILs) derived from the cross between indica restorer line Luhui 99( R99)and super japonica cultivar Shennong 265( SN265) were used to identify the QTLs of cold tolerance at germination and seedling stages. The germination rate of rice seeds and the withered degree of leaves at seedling stage were used as the standards of cold tolerance,QTLs were mapped by QTL IciMapping v3. 0 software. Two QTLs controlling cold tolerance at germination stage and one QTL controlling cold tolerance at seedling stage were detected,which were located on chromosomes 3,5 and 9,respectively,named qLTG-3,qLTG-5 and qSCT-9. The LOD values of the three QTLs were 3. 60,2. 73,and 2. 52,and their additive effects were 0. 09,-0. 10,and-0. 09,respectively,which accounted for 11. 02%,14. 07%,and 12. 18% of phenotypic variation,respectively. Among them,qLTG-5 was located between R5 M13-RS8 markers with a genetic distance of 8. 0 cM on chromosomes 5,and no cold tolerance QTL was reported in this interval,so it might be a new QTL for cold tolerance at germination stage in rice. The results laid the foundation for fine mapping and cloning of related cold tolerance genes.
The aim of the study was to locate the QTL of rice cold tolerance at germination and seedling stages,to identify new locus of cold tolerance gene,and to enrich the molecular genetic basis of rice cold tolerance.144 F8 stable recombinant inbred lines( RILs) derived from the cross between indica restorer line Luhui 99( R99)and super japonica cultivar Shennong 265( SN265) were used to identify the QTLs of cold tolerance at germination and seedling stages. The germination rate of rice seeds and the withered degree of leaves at seedling stage were used as the standards of cold tolerance,QTLs were mapped by QTL IciMapping v3. 0 software. Two QTLs controlling cold tolerance at germination stage and one QTL controlling cold tolerance at seedling stage were detected,which were located on chromosomes 3,5 and 9,respectively,named qLTG-3,qLTG-5 and qSCT-9. The LOD values of the three QTLs were 3. 60,2. 73,and 2. 52,and their additive effects were 0. 09,-0. 10,and-0. 09,respectively,which accounted for 11. 02%,14. 07%,and 12. 18% of phenotypic variation,respectively. Among them,qLTG-5 was located between R5 M13-RS8 markers with a genetic distance of 8. 0 cM on chromosomes 5,and no cold tolerance QTL was reported in this interval,so it might be a new QTL for cold tolerance at germination stage in rice. The results laid the foundation for fine mapping and cloning of related cold tolerance genes.
引文
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[2]苏柏元,陈惠哲,朱德峰.水稻直播栽培技术发展现状及对策[J].农业科技通讯,2014(1):7-11. doi:10. 3969/j. issn. 1000-6400. 2014. 01. 003.Su B Y,Chen H Z,Zhu D F. Development status andcountermeasures of rice direct seeding cultivation technol-ogy[J]. Bulletin of Agricultural Science and Technology,2014(1):7-11. doi:10. 3969/j. issn. 1000-6400.2014. 01. 003.
[3]杨梯丰,张少红,王晓飞,董景芳,黄章慧,赵均良,刘清,毛兴学,张桂权,刘斌.多样性国际稻种4个生长发育时期的耐冷性及其与籼粳性的关系[J].分子植物育种,2017(2):763-773. doi:10. 13271/j.mpb. 015. 000763.Yang T F,Zhang S H,Wang X F,Dong J F,Huang ZH,Zhao J L,Liu Q,Mao X X,Zhang G Q,Liu B. E-valuation of cold tolerance at four growth stages and analy-sis of relationship between cold tolerance and indica-ja-ponica differentiation in a diverse rice collection(Oryzasativa L.)[J]. Molecular Plant Breeding,2017(2):763-773. doi:10. 13271/j. mpb. 015. 000763.
[4]肖宁,李育红,王志平,戴正元,黄年生,潘存红,张小祥,周长海,吴云雨,余玲,钱熀俊,陈建民,李爱宏.水稻耐冷QTL定位以及分子机理研究进展[J].分子植物育种,2016,14(7):1698-1704. doi:10.13271/j. mpb. 014. 001698.Xiao N,Li Y H,Wang Z P,Dai Z Y,Huang N S,PanC H,Zhang X X,Zhou C H,Wu Y Y,Yu L,Qian HJ,Chen J M,Li A H. Research advances on QTL map-ping of cold tolerant and the molecular mechanism in rise[J]. Molecular Plant Breeding,2016,14(7):1698-1704. doi:10. 13271/j. mpb. 014. 001698.
[5]刘次桃,王威,毛毕刚,储成才.水稻耐低温逆境研究:分子生理机制及育种展望[J].遗传,2018,40(3):171-185. doi:10. 16288/j. yczz. 18-007.Liu C L,Wang W,Mao B G,Chu C C. Cold stress tol-erance in rice:physiological changes,molecular mecha-nism,and future prospects[J]. Hereditas,2018,40(3):171-185. doi:10. 16288/j. yczz. 18-007.
[6] Baruah A R,Ishigo-Oka N,Adachi M,Oguma Y,Toki-zono Y,Onishi K,Sano Y. Cold tolerance at the earlygrowth stage in wild and cultivated rice[J]. Euphytica,2009,165(3):459-470. doi:10. 1007/s10681-008-9753-y.
[7]陈玮,李炜.水稻RIL群体芽期耐冷性基因的分子标记定位[J].武汉植物学研究,2005,23(2):116-120. doi:10. 3969/j. issn. 2095-0837. 2005. 02. 004.Chen W,Li H. Mapping of QTL conferring cold toleranceat early seedling stage of rice by molecular markers[J].Journal of Wuhan Botanical Research,2005,23(2):116-120. doi:10. 3969/j. issn. 2095-0837. 2005. 02. 004.
[8]杨洛淼,王敬国,刘化龙,孙健,郑洪亮,邹德堂.寒地粳稻发芽期和芽期的耐冷性QTL定位[J].作物杂志,2014(6):44-51. doi:10. 16035/j. issn. 1001-7283. 2014. 06. 009.Yang L M,Wang J G,Liu H L,Sun J,Zheng H L,ZouD T. QTL mapping of cold tolerance for the germinationperiod and the bud bursting period of japonica in cold are-a[J]. Crops,2014(6):44-51. doi:10. 16035/j. issn.1001-7283. 2014. 06. 009.
[9]周勇,朱孝波,袁华,郑英,钦鹏,魏应海,王玉平,黄世君,李仕贵.水稻单片段代换系芽期和苗期耐冷性分析及耐冷性QTL鉴定[J].中国水稻科学,2013,25(4):381-388. doi:10. 16035/j. issn. 1001-7283.2014. 06. 009.Zhou Y,Zhu X B,Yuan H,Zheng Y,Qin P,Wei Y H,Wang Y P,Huang S J,Li S G. Characterization of coldtolerance and identification of clod tolerance QTLs for ricesingle segment substitution lines at plumule and seedlingstages[J]. Chinese Journal of Rice Science,2013,25(4):381-388. doi:10. 16035/j. issn. 1001-7283. 2014.06. 009.
[10] Zhao J,Qin J J,Song Q,Sun C Q,Liu F X. CombiningQTL mapping and expression profile analysis to identifycandidate genes of cold tolerance from Dongxiang com-mon wild rice(Oryza rufipogon Griff.)[J]. Journal ofIntegrative Agriculture,2016,15(9):1933-1943. doi:10. 1016/S2095-3119(15)61214-X.
[11]王会民,唐秀英,龙起樟,黄永兰,芦明,徐晓明,万建林.一个水稻耐冷主效QTL-qCT10的分离鉴定[J].分子植物育种,2017(2):582-588. doi:10.13271/j. mpb. 015. 000582.Wang H M,Tang X Y,Long Q Z,Huang Y L,Lu M,Xu X M,Wan J L. Identification of q CT10,a majorQTL associated with rice cold tolerance[J]. MolecularPlant Breeding,2017(2):582-588. doi:10. 13271/j.mpb. 015. 000582.
[12]李太贵.在低温下筛选水稻不同生长期耐寒品种的室内方法[J].国外农业技术,1981(4):18-21.Li T G. Indoor method for screening cold-tolerant varie-ties of rice in different growth stages at low temperature[J]. Foreign Agricultural Technology,1981(4):18-21.
[13]韩龙植,张三元.水稻耐冷性鉴定评价方法[J].植物遗传资源学报,2004,5(1):75-80. doi:10.3969/j. issn. 1672-1810. 2004. 01. 017.Han L Z,Zhang S Y. Methods of characterization andevaluation of cold tolerance in rice[J]. Journal of PlantGenetic Resources,2004,5(1):75-80. doi:10. 3969/j. issn. 1672-1810. 2004. 01. 017.
[14]张玲,李晓楠,王伟,杨生龙,李清,王嘉宇.水稻株型相关性状的QTL分析[J].作物学报,2014,40(12):2128-2135. doi:10. 3724/SP. J. 1006. 2014.02128.Zhang L,Li X N,Wang W,Yang S L,Li Q,Wang JY. Analysis of QTLs for plant type traits in rice(Oryzasativa)[J]. Acta Agronomica Sinica,2014,40(12):2128-2135. doi:10. 3724/SP. J. 1006. 2014. 02128.
[15] Wang J K,Li H H,Zhang L. Users'Manual of QTLICI Mapping v3. 0 2011. Webpage:http//www. isbreed-ing. net.
[16] Mc Couch S R,Cho Y G,Yano M. Report on QTL nomen-clature[J]. Rice Genet Newsletter,1997,14:11-13.
[17] Zhang Z Y,Li J J,Pan Y H,Li J L,Zhou L,Shi H L,Zeng Y W,Guo H F,Yang S M,Zheng W W,Yu J P,Sun X M,Li G L,Ding Y L,Ma L,Shen S Q,Dai YL,Zhang H L,Yang S H,Guo Y,Li Z C. Natural var-iation in CTB4a enhances rice adaptation to cold habitats[J]. Nat Commun,2017,8(3):14788. doi:10.1038/ncomms14788.
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